Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)

Detalhes bibliográficos
Autor(a) principal: Carvalho, Tatiana Borges de
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5119
Resumo: Mammary tumor is the most frequent neoplasms in female dogs and the similarity of those tumors with the mammary tumors in the woman determines an interest in the study of the compared pathology. The expression of Heat Shock Proteins (HSP) have been observed in many neoplasms, including breast cancer. The aim of this work was to classify histopatologically and to investigate the expression of HSP 72 in normal and neoplastic canine mammary glands of female dogs assisted in the service of the Veterinary Hospital of the Federal University of Viçosa. 34 samples of mammary tissue were collected, including 23 neoplastics and 11 normal tissue, for histopatological analysis and investigation of expression of HSP 72 using Western blotting. Furthermore, the variables age to the diagnosis, breed, attacked mammary glands and histopatological classification of the tissue were investigated. Statistical analysis was carried out using Chi-square test (&#967;²). The result were considered statistically significant in the P < 0,05. The major frequency of the neoplasm diagnostic was at 8 to 13 years old, at the inguinal and caudal abdominal mammary glands and no statistical difference was observed between malignants and benign tumors nor at histopatological classifications. The expression of HSP 72 was determined in normal and neoplastic mammary gland tissue, this last one presenting an accentuated expression of this protein. The neoplastic tissue presented a larger proportion of expression of the protein HSP 72 when compared to the normal tissue, being observed equivalence among benign and malignants tumors as for the expression of that protein.
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spelling Carvalho, Tatiana Borges dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4130410J7Dias, Cristina Maria Ganns Chaveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786741P6Carlo, Ricardo Junqueira Delhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788143A9Borges, Andrea Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767373D8Fontes, Elizabeth Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2Natali, Antônio Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795725H42015-03-26T13:47:09Z2007-04-172015-03-26T13:47:09Z2006-09-28CARVALHO, Tatiana Borges de. Mammary neoplasms of female dogs: histopatological characterization and expression of stress proteins (HSP 72). 2006. 49 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/5119Mammary tumor is the most frequent neoplasms in female dogs and the similarity of those tumors with the mammary tumors in the woman determines an interest in the study of the compared pathology. The expression of Heat Shock Proteins (HSP) have been observed in many neoplasms, including breast cancer. The aim of this work was to classify histopatologically and to investigate the expression of HSP 72 in normal and neoplastic canine mammary glands of female dogs assisted in the service of the Veterinary Hospital of the Federal University of Viçosa. 34 samples of mammary tissue were collected, including 23 neoplastics and 11 normal tissue, for histopatological analysis and investigation of expression of HSP 72 using Western blotting. Furthermore, the variables age to the diagnosis, breed, attacked mammary glands and histopatological classification of the tissue were investigated. Statistical analysis was carried out using Chi-square test (&#967;²). The result were considered statistically significant in the P < 0,05. The major frequency of the neoplasm diagnostic was at 8 to 13 years old, at the inguinal and caudal abdominal mammary glands and no statistical difference was observed between malignants and benign tumors nor at histopatological classifications. The expression of HSP 72 was determined in normal and neoplastic mammary gland tissue, this last one presenting an accentuated expression of this protein. The neoplastic tissue presented a larger proportion of expression of the protein HSP 72 when compared to the normal tissue, being observed equivalence among benign and malignants tumors as for the expression of that protein.Tumor de mama é a neoplasia mais freqüente em cadelas e a semelhança com os tumores da mama na mulher determina um interesse crescente no estudo da patologia comparada. A expressão de proteínas de choque térmico (HSP) tem sido observada em muitas neoplasias, incluindo o câncer de mama. O objetivo desse trabalho foi classificar histopatologicamente e investigar a expressão de HSP 72 em glândula mamária normal e neoplásica de cadelas atendidas no serviço ambulatorial do Hospital Veterinário da Universidade Federal de Viçosa. Foram utilizadas 34 amostras de tecido mamário, sendo 23 neoplásicas e 11 normais, submetidas à análise histopatológica e pesquisa de HSP 72 através de Western blotting. Além disso, foram investigadas as variáveis: idade ao diagnóstico, raça, glândulas mamárias acometidas e classificação histopatológica da amostra. Os dados foram analisados por meio de tabelas de freqüência e a comparação entre as proporções das variáveis pelo teste de Qui-quadrado (&#967;²), utilizando nível de significância de 5%. Observou-se maior frequência de diagnóstico da neoplasia mamária em cadelas entre 8 e 13 anos de idade, nas glândulas mamárias abdominais caudais e inguinais, sem diferença estatística entre malignas e benignas e classificação histopatológica. Os tecidos neoplásicos apresentaram uma maior proporção de expressão da proteína HSP 72 quando comparados ao tecido normal, sendo observado equivalência entre tumores benignos e malignos quanto à expressão dessa proteína.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deNeoplasia mamáriaProteína de estresseCãoMammary neoplasmsStress proteinsDogsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMALNeoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)Mammary neoplasms of female dogs: histopatological characterization and expression of stress proteins (HSP 72)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf226846https://locus.ufv.br//bitstream/123456789/5119/1/texto%20completo.pdffab823cf7946385dda72c2e97936a8ecMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain74023https://locus.ufv.br//bitstream/123456789/5119/2/texto%20completo.pdf.txt03036af0090aa78fab19f2e5d1ed8150MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3518https://locus.ufv.br//bitstream/123456789/5119/3/texto%20completo.pdf.jpg4daaeef8200c46326a756a9b51898385MD53123456789/51192016-04-11 23:09:08.549oai:locus.ufv.br:123456789/5119Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:09:08LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
dc.title.alternative.eng.fl_str_mv Mammary neoplasms of female dogs: histopatological characterization and expression of stress proteins (HSP 72)
title Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
spellingShingle Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
Carvalho, Tatiana Borges de
Neoplasia mamária
Proteína de estresse
Cão
Mammary neoplasms
Stress proteins
Dogs
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
title_short Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
title_full Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
title_fullStr Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
title_full_unstemmed Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
title_sort Neoplasia mamária em cadelas: caracterização histopatológica e expressão de proteínas de estresse (HSP 72)
author Carvalho, Tatiana Borges de
author_facet Carvalho, Tatiana Borges de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4130410J7
dc.contributor.author.fl_str_mv Carvalho, Tatiana Borges de
dc.contributor.advisor-co1.fl_str_mv Dias, Cristina Maria Ganns Chaves
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786741P6
dc.contributor.advisor-co2.fl_str_mv Carlo, Ricardo Junqueira Del
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788143A9
dc.contributor.advisor1.fl_str_mv Borges, Andrea Pacheco Batista
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767373D8
dc.contributor.referee1.fl_str_mv Fontes, Elizabeth Pacheco Batista
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2
dc.contributor.referee2.fl_str_mv Natali, Antônio José
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795725H4
contributor_str_mv Dias, Cristina Maria Ganns Chaves
Carlo, Ricardo Junqueira Del
Borges, Andrea Pacheco Batista
Fontes, Elizabeth Pacheco Batista
Natali, Antônio José
dc.subject.por.fl_str_mv Neoplasia mamária
Proteína de estresse
Cão
topic Neoplasia mamária
Proteína de estresse
Cão
Mammary neoplasms
Stress proteins
Dogs
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
dc.subject.eng.fl_str_mv Mammary neoplasms
Stress proteins
Dogs
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
description Mammary tumor is the most frequent neoplasms in female dogs and the similarity of those tumors with the mammary tumors in the woman determines an interest in the study of the compared pathology. The expression of Heat Shock Proteins (HSP) have been observed in many neoplasms, including breast cancer. The aim of this work was to classify histopatologically and to investigate the expression of HSP 72 in normal and neoplastic canine mammary glands of female dogs assisted in the service of the Veterinary Hospital of the Federal University of Viçosa. 34 samples of mammary tissue were collected, including 23 neoplastics and 11 normal tissue, for histopatological analysis and investigation of expression of HSP 72 using Western blotting. Furthermore, the variables age to the diagnosis, breed, attacked mammary glands and histopatological classification of the tissue were investigated. Statistical analysis was carried out using Chi-square test (&#967;²). The result were considered statistically significant in the P < 0,05. The major frequency of the neoplasm diagnostic was at 8 to 13 years old, at the inguinal and caudal abdominal mammary glands and no statistical difference was observed between malignants and benign tumors nor at histopatological classifications. The expression of HSP 72 was determined in normal and neoplastic mammary gland tissue, this last one presenting an accentuated expression of this protein. The neoplastic tissue presented a larger proportion of expression of the protein HSP 72 when compared to the normal tissue, being observed equivalence among benign and malignants tumors as for the expression of that protein.
publishDate 2006
dc.date.issued.fl_str_mv 2006-09-28
dc.date.available.fl_str_mv 2007-04-17
2015-03-26T13:47:09Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:47:09Z
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dc.identifier.citation.fl_str_mv CARVALHO, Tatiana Borges de. Mammary neoplasms of female dogs: histopatological characterization and expression of stress proteins (HSP 72). 2006. 49 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5119
identifier_str_mv CARVALHO, Tatiana Borges de. Mammary neoplasms of female dogs: histopatological characterization and expression of stress proteins (HSP 72). 2006. 49 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
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