Synthetic vaccine (SBm7462) against the cattle tick Rhipicephalus (Boophilus) microplus: Preservation of immunogenic determinants in different strains from South America

• Autor(a) principal: Peconick, A.P.
• Data de Publicação: 2007
• Outros Autores: Sossai, S., Girão, F.A., Rodrigues, M.Q.R.B., Silva, C.H. Souza e, Guzman Q, F., Patarroyo V, A.M., Vargas, M.I., Patarroyo, J.H.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.exppara.2007.12.007; http://www.locus.ufv.br/handle/123456789/19572
• topic: Rhipicephalus (Boophilus) microplus; Tick; Protein Bm86; Protein Bm95; Conserved epitope sequences; Synthetic vaccine; Polymerase chain reaction (PCR); Base pair (bp); Amino acids (a.a.)

The synthetic vaccine SBm7462 is based on three immunogenic epitopes (4822, 4823 and 4824) contained within protein Bm86 derived from the Australian Yeerongpilly strain of the tick Rhipicephalus (Boophilus) microplus. Twenty strains of the tick originating from Brazil, Argentina, Colombia and Uruguay were analysed in order to identify differences compared with sequences present in components of vaccine SBm7462. For each parasite population, three cDNA fragments containing the nucleotides coding for the epitopes 4822, 4824 and 4823 were sequenced, and the amino acid sequences were deduced and compared with those of the homologous bm86 gene. The results indicate that the epitope sequences of vaccine SBm7462 are conserved in the South American populations of the tick. The conservation of such sequences is very important for the immunological response of different populations of R. (B.) microplus.