Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/5717 |
Resumo: | In Brazil, the dairy goat industry is characterized by a large number of low technology farms, with an average daily milk production per goat of 1 Kg. Semen biotechnologies as artificial insemination (AI) can be an important tool to improve the genetic gain and development of these farms. The use of frozen semen in IA protocols requires a higher level of technology and costs for the farmer when compared with the use of cooled or fresh semen; besides the fertility results from field trials using frozen semen are still doubtful. However the use of fresh semen is restrict to a short period after colleted as the viability of the cells decrease fast at environmental temperatures. Therefore the use of cooled semen in low level technology farms can result in a less-expensive and more efficient tool for multiplying the genetic material in these farms. The aim of this study was to evaluate the fertility in vivo of goat semen cooled and stored at 5˚ Celsius for 24 hours. The experiment was realized in the Goat Research Center of the Animal Science Department/Federal University of Vicosa, Brazil, from May until July (end of the natural breeding season) of 2005. Animals from the Alpine (n=2) and Saanen (n=2) breed were utilized as semen donors and a total of 71 females (lactating goats and young goats) from both breeds were used in the fertility trial. Semen was collected by artificial vagina method, diluted in Ringer Lactate solution and centrifuged at 420 G for 10 minutes. After, the semen pellet was rediluted in Egg-yolk (20% v/v) Citrate extender to achieve a total of 400x106 spermatozoa/ mL. The semen was stored in 0.25 French straws and then used immediately for AI or cooled (average cooling rate = -0.5 ˚C/minute) for 1 hour and stored at 5˚ C for 24 hours before its use for IA. The animals were bred with fresh or cooled semen at 12 hours after the on set of estrus; if the animals continued in estrus for more than 12 hours a second IA was realize at 24 hours after the first insemination. Pregnancy diagnosis was realized by transrectal ultrasonography using a real time B mode scanner with a 5 MHZ linear array transducer. The ultrasonography evaluations were done at day 45 after first IA. The conception rate for young goats (31.2 x 20,0%) and goats (44.4 x 17.6%) did not differ (P>0.05) between fresh or cooled semen, respectively. The total conception rate did not differ (P>0.05) between fresh (38.2%) and cooled (18.9%) semen. The average conception rate of this same herd and period was 28.2%. Historical data from the last four years in the same experimental period and farm showed an average conception rate of 39,9% (109/273). The present study didn't find differences in the conception rates for the semen diluted in Yolk-Citrate with 20% of Egg-Yolk and used to fresh or cold and stored by 24 hours to 5ºC, however, the fertility of the semen cold was below that obtained with controlled breed in the end of the natural reproductive station. Factors climatic, environmental and of handling, besides the quality of the animals, related with the end of the reproductive station, might have contributed to the low fertilities found in the present study. |
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Palhão, Miller Pereirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4753653D8Torres, Ciro Alexandre Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4Rodrigues, Marcelo Teixeirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788161Y5Carvalho, Giovanni Ribeiro dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6Costa, Eduardo Paulino dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6Fonseca, Jeferson Ferreira dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703690H82015-03-26T13:55:08Z2007-02-122015-03-26T13:55:08Z2006-08-11PALHÃO, Miller Pereira. Evaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hours. 2006. 81 f. Dissertação (Mestrado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/5717In Brazil, the dairy goat industry is characterized by a large number of low technology farms, with an average daily milk production per goat of 1 Kg. Semen biotechnologies as artificial insemination (AI) can be an important tool to improve the genetic gain and development of these farms. The use of frozen semen in IA protocols requires a higher level of technology and costs for the farmer when compared with the use of cooled or fresh semen; besides the fertility results from field trials using frozen semen are still doubtful. However the use of fresh semen is restrict to a short period after colleted as the viability of the cells decrease fast at environmental temperatures. Therefore the use of cooled semen in low level technology farms can result in a less-expensive and more efficient tool for multiplying the genetic material in these farms. The aim of this study was to evaluate the fertility in vivo of goat semen cooled and stored at 5˚ Celsius for 24 hours. The experiment was realized in the Goat Research Center of the Animal Science Department/Federal University of Vicosa, Brazil, from May until July (end of the natural breeding season) of 2005. Animals from the Alpine (n=2) and Saanen (n=2) breed were utilized as semen donors and a total of 71 females (lactating goats and young goats) from both breeds were used in the fertility trial. Semen was collected by artificial vagina method, diluted in Ringer Lactate solution and centrifuged at 420 G for 10 minutes. After, the semen pellet was rediluted in Egg-yolk (20% v/v) Citrate extender to achieve a total of 400x106 spermatozoa/ mL. The semen was stored in 0.25 French straws and then used immediately for AI or cooled (average cooling rate = -0.5 ˚C/minute) for 1 hour and stored at 5˚ C for 24 hours before its use for IA. The animals were bred with fresh or cooled semen at 12 hours after the on set of estrus; if the animals continued in estrus for more than 12 hours a second IA was realize at 24 hours after the first insemination. Pregnancy diagnosis was realized by transrectal ultrasonography using a real time B mode scanner with a 5 MHZ linear array transducer. The ultrasonography evaluations were done at day 45 after first IA. The conception rate for young goats (31.2 x 20,0%) and goats (44.4 x 17.6%) did not differ (P>0.05) between fresh or cooled semen, respectively. The total conception rate did not differ (P>0.05) between fresh (38.2%) and cooled (18.9%) semen. The average conception rate of this same herd and period was 28.2%. Historical data from the last four years in the same experimental period and farm showed an average conception rate of 39,9% (109/273). The present study didn't find differences in the conception rates for the semen diluted in Yolk-Citrate with 20% of Egg-Yolk and used to fresh or cold and stored by 24 hours to 5ºC, however, the fertility of the semen cold was below that obtained with controlled breed in the end of the natural reproductive station. Factors climatic, environmental and of handling, besides the quality of the animals, related with the end of the reproductive station, might have contributed to the low fertilities found in the present study.Em nosso país, a caprinocultura leiteira é praticada em propriedades pouco tecnificadas, com produção média em torno de 1 kg de leite/cabra/dia. A utilização da inseminação artificial como ferramenta para o melhoramento genético é dependente dos resultados obtidos nos testes de fertilidade a campo. A utilização do sêmen congelado, além de esbarrar nos problemas de custos, ainda apresenta resultados contraditórios, sendo sua utilização limitada a poucos rebanhos matrizeiros. Já o sêmen fresco tem a limitação do curto tempo de viabilidade, ficando seu uso restrito a própria propriedade. Neste sentido a utilização do sêmen resfriado pode servir de alternativa barata para a multiplicação daqueles reprodutores de maior potencial genético. Assim, o presente estudo visou testar fertilidade in vivo do sêmen caprino, diluído em um extensor a base de citrato-gema e armazenado a 5ºC por 24 horas. Para tanto, conduziu-se um experimento durante os meses de maio de junho de 2005, no setor de Caprinocultura do DZO/UFV, o qual contou com quatro bodes e 71 cabras e cabritas, das raças Saanen e Alpina. O sêmen foi coletado em vagina artificial, imediatamente centrifugado em meio Ringer Lactato, a 402G por 10 minutos, em seguida o sobrenadante descartado e as células re-suspendidas em meio diluidor a base de citrato-gema, com 20% de gema de ovo, perfazendo um total de 100x106 espermatozóides móveis em um volume de 0,25 mL. Após o envase, metade das doses permanecia na bancada e a outra metade era submetida ao processo de resfriamento, com uma curva de aproximadamente 0,5ºC/minuto, atingindo a temperatura de geladeira 5ºC em 1 hora. De acordo com a ordem de manifestação do estro os animais entravam recebiam duas inseminações intervaladas de 24 horas, sendo a primeira realizada 12 horas após a observação do estro, com sêmen fresco ou resfriado. O diagnóstico de gestação foi realizado aos 45 dias, com auxilio de um ultra-som equipado com uma probe de 5 MHz de freqüência. Não houve diferenças significativas (P>0,05), quanto a fertilidade das cabritas foi de 31,2% (5/16) e 20,0% (4/20), e nem das cabras 44,4% e 17,6%, inseminadas com sêmen fresco e resfriado, respectivamente. Somando cabras e cabritas a fertilidade foi de 38,2% e 18,9%, respectivamente para o tratamento a fresco e resfriado, não sendo significativa a diferença (P>0,05). A taxa de concepção geral do experimento foi de 28,2%. Na mesma época do ano, a fertilidade do rebanho em um levantamento dos últimos quatro anos foi de 39,9% (109/273), podendo indicar problemas de fertilidade relacionados às fêmeas. O presente estudo não encontrou diferenças na taxa de concepção para o sêmen diluído em Citrato-Gema com 20% de Gema de ovo e utilizado a fresco ou resfriado e armazenado por 24 horas a 5ºC, no entanto, a fertilidade do sêmen resfriado ficou abaixo daquela obtida com a monta controlada no final da estação reprodutiva natural. Fatores climáticos, ambientais e de manejo, além da qualidade zootécnica dos animais, relacionada com o final da estação reprodutiva, podem ter contribuído para as baixas fertilidades encontradas no presente estudo.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em ZootecniaUFVBRGenética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e ForragiculSêmen caprino resfriadoInseminação artificialDiluidor citrato-gemaCooled goat semenArtificial inseminationDiluted egg-yolk citrateCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMALAvaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horasAvaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horasEvaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hoursEvaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hoursinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf621878https://locus.ufv.br//bitstream/123456789/5717/1/texto%20completo.pdfa616ae227883c57266ba5606898260c2MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain141618https://locus.ufv.br//bitstream/123456789/5717/2/texto%20completo.pdf.txt64a349be0efbdefbbd6e4ebad51886afMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3688https://locus.ufv.br//bitstream/123456789/5717/3/texto%20completo.pdf.jpg9fedd00c1a1011801e594f919b513b4dMD53123456789/57172016-04-10 23:15:44.173oai:locus.ufv.br:123456789/5717Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:15:44LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hours Evaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hours |
title |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
spellingShingle |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas Palhão, Miller Pereira Sêmen caprino resfriado Inseminação artificial Diluidor citrato-gema Cooled goat semen Artificial insemination Diluted egg-yolk citrate CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL |
title_short |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
title_full |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
title_fullStr |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
title_full_unstemmed |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
title_sort |
Avaliação do sêmen caprino diluído em citrato-gema, resfriado e armazenado a 5ºC por 24 horas |
author |
Palhão, Miller Pereira |
author_facet |
Palhão, Miller Pereira |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4753653D8 |
dc.contributor.author.fl_str_mv |
Palhão, Miller Pereira |
dc.contributor.advisor-co1.fl_str_mv |
Torres, Ciro Alexandre Alves |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4 |
dc.contributor.advisor-co2.fl_str_mv |
Rodrigues, Marcelo Teixeira |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788161Y5 |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Giovanni Ribeiro de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6 |
dc.contributor.referee1.fl_str_mv |
Costa, Eduardo Paulino da |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6 |
dc.contributor.referee2.fl_str_mv |
Fonseca, Jeferson Ferreira da |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703690H8 |
contributor_str_mv |
Torres, Ciro Alexandre Alves Rodrigues, Marcelo Teixeira Carvalho, Giovanni Ribeiro de Costa, Eduardo Paulino da Fonseca, Jeferson Ferreira da |
dc.subject.por.fl_str_mv |
Sêmen caprino resfriado Inseminação artificial Diluidor citrato-gema |
topic |
Sêmen caprino resfriado Inseminação artificial Diluidor citrato-gema Cooled goat semen Artificial insemination Diluted egg-yolk citrate CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL |
dc.subject.eng.fl_str_mv |
Cooled goat semen Artificial insemination Diluted egg-yolk citrate |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL |
description |
In Brazil, the dairy goat industry is characterized by a large number of low technology farms, with an average daily milk production per goat of 1 Kg. Semen biotechnologies as artificial insemination (AI) can be an important tool to improve the genetic gain and development of these farms. The use of frozen semen in IA protocols requires a higher level of technology and costs for the farmer when compared with the use of cooled or fresh semen; besides the fertility results from field trials using frozen semen are still doubtful. However the use of fresh semen is restrict to a short period after colleted as the viability of the cells decrease fast at environmental temperatures. Therefore the use of cooled semen in low level technology farms can result in a less-expensive and more efficient tool for multiplying the genetic material in these farms. The aim of this study was to evaluate the fertility in vivo of goat semen cooled and stored at 5˚ Celsius for 24 hours. The experiment was realized in the Goat Research Center of the Animal Science Department/Federal University of Vicosa, Brazil, from May until July (end of the natural breeding season) of 2005. Animals from the Alpine (n=2) and Saanen (n=2) breed were utilized as semen donors and a total of 71 females (lactating goats and young goats) from both breeds were used in the fertility trial. Semen was collected by artificial vagina method, diluted in Ringer Lactate solution and centrifuged at 420 G for 10 minutes. After, the semen pellet was rediluted in Egg-yolk (20% v/v) Citrate extender to achieve a total of 400x106 spermatozoa/ mL. The semen was stored in 0.25 French straws and then used immediately for AI or cooled (average cooling rate = -0.5 ˚C/minute) for 1 hour and stored at 5˚ C for 24 hours before its use for IA. The animals were bred with fresh or cooled semen at 12 hours after the on set of estrus; if the animals continued in estrus for more than 12 hours a second IA was realize at 24 hours after the first insemination. Pregnancy diagnosis was realized by transrectal ultrasonography using a real time B mode scanner with a 5 MHZ linear array transducer. The ultrasonography evaluations were done at day 45 after first IA. The conception rate for young goats (31.2 x 20,0%) and goats (44.4 x 17.6%) did not differ (P>0.05) between fresh or cooled semen, respectively. The total conception rate did not differ (P>0.05) between fresh (38.2%) and cooled (18.9%) semen. The average conception rate of this same herd and period was 28.2%. Historical data from the last four years in the same experimental period and farm showed an average conception rate of 39,9% (109/273). The present study didn't find differences in the conception rates for the semen diluted in Yolk-Citrate with 20% of Egg-Yolk and used to fresh or cold and stored by 24 hours to 5ºC, however, the fertility of the semen cold was below that obtained with controlled breed in the end of the natural reproductive station. Factors climatic, environmental and of handling, besides the quality of the animals, related with the end of the reproductive station, might have contributed to the low fertilities found in the present study. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-08-11 |
dc.date.available.fl_str_mv |
2007-02-12 2015-03-26T13:55:08Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:55:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PALHÃO, Miller Pereira. Evaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hours. 2006. 81 f. Dissertação (Mestrado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2006. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/5717 |
identifier_str_mv |
PALHÃO, Miller Pereira. Evaluation of the goat semen diluted in egg-yolk citrate, cooled and stored at 5ºC for 24 hours. 2006. 81 f. Dissertação (Mestrado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2006. |
url |
http://locus.ufv.br/handle/123456789/5717 |
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Universidade Federal de Viçosa |
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Mestrado em Zootecnia |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
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Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul |
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Universidade Federal de Viçosa |
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