Influência do laser arseneto de gálio e arseneto de gálio alumínio e pomada cicatrizante em feridas cutâneas de ratos Wistar

• Autor(a) principal: Gonçalves, Reggiani Vilela
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2379
• topic: Cicatrização; Laser Hélio-Neônio; Laser arseneto de gálio e alumínio; Healing; Gallium aluminum arsenide laser; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The present work had as objective evaluates the healing effect of the gallium aluminum arsenide (GaAsAl) 830nm (30J/cm2) (60J/cm2) laser and gallium arsenide (GaAs) 904nm (4J/cm2) laser, comparing with the Dersani® ointment. The analyzed parameters were the amount of type I and III collagen fibers, and the area of contraction of the wound in the different treatments. They were also analyzed the influence of the different treatments on the weight and the hematologic values of the animals. They were used 30 Wistar rats, young adults, males, with medium weight of 324g, separate in five groups, each group with six animals. The animals of the group 1 were treated with GaAs (4J/cm2) laser, the animals of the group 2 treated with the GaAsAl (30J/cm2) laser, in the group 3 the animals received radiation of the GaAsAl (60J/cm2) laser, in the group 4 the animals received the topical treatment with the Dersani® ointment and in the group 5 the animals had the wound treated with saline. Each animal had the back waxed and were made five standardized wounds, previously marking the area and removing the skin. The material for histological analyze was dyed with picrosirius, photographed and the pictures analyzed in the program Quantum, based on the primary colors (Red, Green, Blue). It was observed a significant statistical difference of the weigth of the animals in relation to the different treatments and the time of the experiment. Considering the area of the wound, the best results for the closing of the wound were obtained of the groups treated with the GaAs (4J/cm2) laser and GaAsAl (60J/cm2) laser. The leukocyte global count in the different treatment didn t present significant difference, except in the animals treated with Dersani® where there was significant increase in the number of monocytes. The treatments with saline and GaAsAl (30J/cm2) laser presented increase in the number of neutrophils. The larger number of type III collagen fibers was observed in the animals irradiated with the GaAsAl (30J/cm2) laser. In relationship to the type I collagen fibers, the different treatments didn t present a significant increase. It was observed then that the GaAsAl (30J/cm2) laser was effective in the production of type III collagen fibers and in the cicatricial process and that the GaAs (4J/cm2) laser it went effective to promote the closing of the area of the wound.