Bovine mature adipocytes readily return to a proliferative state

• Autor(a) principal: Duarte, M. S.
• Data de Publicação: 2012
• Outros Autores: Wei, S., Du, M., Paulino, P. V. R., Jiang, Z., Albrecht, E., Fernyhough-Culver, M., Zan, L., Hausman, G. J., Dodson, M. V.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.tice.2012.08.001; http://www.locus.ufv.br/handle/123456789/22067
• topic: Mature adipocytes; Proliferation; DFAT cells

The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for studying the development and biology of adipocytes. In this research, we show that isolated (and purified) mature adipocytes derived from Wagyu cattle dedifferentiate into progeny cells, and that these spindle-shaped, proliferative-competent daughter cells possess ability to proliferate. We outline the optimum cell culture system and offer precautionary thoughts for effective mature adipocyte culture. Collectively, this represents a novel cell model which may provide new insights into cell development, physiology and use as a model for animal production/composition, tissue engineering and disease treatment.