Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA

Detalhes bibliográficos
Autor(a) principal: Sakiyama, Ney S.
Data de Publicação: 2007
Outros Autores: Vallejos, C. Eduardo, Jones, Valerie, Shanker, Savita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1007/s11105-007-0001-8
http://www.locus.ufv.br/handle/123456789/22624
Resumo: We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.
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spelling Sakiyama, Ney S.Vallejos, C. EduardoJones, ValerieShanker, Savita2018-11-28T18:41:32Z2018-11-28T18:41:32Z2007-07-2515729818http://dx.doi.org/10.1007/s11105-007-0001-8http://www.locus.ufv.br/handle/123456789/22624We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.engPlant Molecular Biology ReporterVolume 25, Issue 1– 2, Pages 11– 16, June 2007Springer-Verlag 2007info:eu-repo/semantics/openAccessBAC DNA purificationDirect BAC DNA sequencingBAC fingerprintingAdaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf237173https://locus.ufv.br//bitstream/123456789/22624/1/artigo.pdfe88c0352a51d6d7928558be1db6f3bbeMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22624/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/226242018-11-28 15:58:43.937oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-11-28T18:58:43LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
title Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
spellingShingle Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
Sakiyama, Ney S.
BAC DNA purification
Direct BAC DNA sequencing
BAC fingerprinting
title_short Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
title_full Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
title_fullStr Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
title_full_unstemmed Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
title_sort Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
author Sakiyama, Ney S.
author_facet Sakiyama, Ney S.
Vallejos, C. Eduardo
Jones, Valerie
Shanker, Savita
author_role author
author2 Vallejos, C. Eduardo
Jones, Valerie
Shanker, Savita
author2_role author
author
author
dc.contributor.author.fl_str_mv Sakiyama, Ney S.
Vallejos, C. Eduardo
Jones, Valerie
Shanker, Savita
dc.subject.pt-BR.fl_str_mv BAC DNA purification
Direct BAC DNA sequencing
BAC fingerprinting
topic BAC DNA purification
Direct BAC DNA sequencing
BAC fingerprinting
description We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.
publishDate 2007
dc.date.issued.fl_str_mv 2007-07-25
dc.date.accessioned.fl_str_mv 2018-11-28T18:41:32Z
dc.date.available.fl_str_mv 2018-11-28T18:41:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11105-007-0001-8
http://www.locus.ufv.br/handle/123456789/22624
dc.identifier.issn.none.fl_str_mv 15729818
identifier_str_mv 15729818
url http://dx.doi.org/10.1007/s11105-007-0001-8
http://www.locus.ufv.br/handle/123456789/22624
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 25, Issue 1– 2, Pages 11– 16, June 2007
dc.rights.driver.fl_str_mv Springer-Verlag 2007
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Springer-Verlag 2007
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plant Molecular Biology Reporter
publisher.none.fl_str_mv Plant Molecular Biology Reporter
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/22624/1/artigo.pdf
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repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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