Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/367 |
Resumo: | The analyses of cyanobacterial communities in soil, lakes and glacial streams samples of three areas within the Maritime Antarctic domain revealed 37 cyanobacterial taxa, with majority belonging to Oscillatoriales Order. The most widespread organisms belonged to genera Phormidium spp,. Leptolyngbya spp. and Nostoc spp.. Seventeen strains were isolated and kept in culture into the Banco de Algas do Laboratório de Ficologia DBV/UFV. Given the concern about the impact of the Antarctic environmental contamination with petroleum hydrocarbons on microbial communities, we tested the effect of three aromatic hydrocarbons on the growth of Phormidium autumnale UFV-ANT01. Toxicological data such as NOEC, EC20 and EC50 were estimated. The toxicity observed on the growth of cyanobacteria tested followed the order: α-benzo-pyrene> anthracene> naphthalene. Concentrations of benzo-α-pyrene four hundred and fifty times smaller than those of naphthalene are sufficient to cause the same toxicity on Phormidium autumnale UFV-ANT01. Populations of cyanobacteria can be a useful tool for the biomonitoring of areas contaminated with hydrocarbons.Bioremediation can be a low cost and ecologic alternative to solve hydrocarbon contamination problems in Antarctic soils. The ability of three cyanobacterial strains in degrading [14C]naphthalene was tested under 15°C. Cyanobacterial lineages Phormidium autumnale UFV-ANT01, Nostoc sp. UFV-ANT23 and Tolypothrix sp. UFV-ANT22 was selected due its important contribution to Antarctic microbial mats. Cyanobateria was investigated in monoclonal cultures and in consortia with heterotrophic bacteria, in comparison with bacterial and abiotic controls. After 200 hours of exposure to labeled hydrocarbons, treatments corresponding to consortia proved to be more effective in [14C]naphthalene than the monoclonal treatments and controls. Cumulative mineralization rates were 42%, 39% and 32%, for Nostoc consortia, Phormidium consortia and Tolypothrix consortia respectively, while bacterial control mineralized 11% of the added hydrocarbon. Monoclonal treatments degraded 2,6 to 4,2% of naphthalene added and were considered less effective than bacterial control, but did not differ from abiotic control (1,9%). Degradation kinetics was similar to those previously reported by other authors for low hydrocarbon concentrations models, in which microbial hydrocarbon attack begins shortly after the contaminant was added to treatments. The rates of hydrocarbon degradation observed to consortia treatments were superior to the sum of individual monoclonal ones and the bacterial control, indicating that cyanobacteria had played a important role in the biodegradation process, providing moisture, nutrients, oxygen and immobilizating the biodegraders microorganisms. |
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Corrêa, Débora Machadohttp://lattes.cnpq.br/9423333212802361Albuquerque, Míriam Abreuhttp://lattes.cnpq.br/7854974657645438Nascimento, Antonio Galvão dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E8Schaefer, Carlos Ernesto Gonçalves Reynaudhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723204Y8Silva, Silvana de Queirozhttp://lattes.cnpq.br/1288764472574843Fernandes, Péricles Leonardohttp://lattes.cnpq.br/97813851351181982015-03-26T12:19:23Z2013-11-282015-03-26T12:19:23Z2012-02-29CORRÊA, Débora Machado. Morphological and molecular characterization of cyanobacteria from Maritme Antarctic and potential on hydrocarbon biodegradation and bioindication in contaminated environments. 2012. 74 f. Tese (Doutorado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/367The analyses of cyanobacterial communities in soil, lakes and glacial streams samples of three areas within the Maritime Antarctic domain revealed 37 cyanobacterial taxa, with majority belonging to Oscillatoriales Order. The most widespread organisms belonged to genera Phormidium spp,. Leptolyngbya spp. and Nostoc spp.. Seventeen strains were isolated and kept in culture into the Banco de Algas do Laboratório de Ficologia DBV/UFV. Given the concern about the impact of the Antarctic environmental contamination with petroleum hydrocarbons on microbial communities, we tested the effect of three aromatic hydrocarbons on the growth of Phormidium autumnale UFV-ANT01. Toxicological data such as NOEC, EC20 and EC50 were estimated. The toxicity observed on the growth of cyanobacteria tested followed the order: α-benzo-pyrene> anthracene> naphthalene. Concentrations of benzo-α-pyrene four hundred and fifty times smaller than those of naphthalene are sufficient to cause the same toxicity on Phormidium autumnale UFV-ANT01. Populations of cyanobacteria can be a useful tool for the biomonitoring of areas contaminated with hydrocarbons.Bioremediation can be a low cost and ecologic alternative to solve hydrocarbon contamination problems in Antarctic soils. The ability of three cyanobacterial strains in degrading [14C]naphthalene was tested under 15°C. Cyanobacterial lineages Phormidium autumnale UFV-ANT01, Nostoc sp. UFV-ANT23 and Tolypothrix sp. UFV-ANT22 was selected due its important contribution to Antarctic microbial mats. Cyanobateria was investigated in monoclonal cultures and in consortia with heterotrophic bacteria, in comparison with bacterial and abiotic controls. After 200 hours of exposure to labeled hydrocarbons, treatments corresponding to consortia proved to be more effective in [14C]naphthalene than the monoclonal treatments and controls. Cumulative mineralization rates were 42%, 39% and 32%, for Nostoc consortia, Phormidium consortia and Tolypothrix consortia respectively, while bacterial control mineralized 11% of the added hydrocarbon. Monoclonal treatments degraded 2,6 to 4,2% of naphthalene added and were considered less effective than bacterial control, but did not differ from abiotic control (1,9%). Degradation kinetics was similar to those previously reported by other authors for low hydrocarbon concentrations models, in which microbial hydrocarbon attack begins shortly after the contaminant was added to treatments. The rates of hydrocarbon degradation observed to consortia treatments were superior to the sum of individual monoclonal ones and the bacterial control, indicating that cyanobacteria had played a important role in the biodegradation process, providing moisture, nutrients, oxygen and immobilizating the biodegraders microorganisms.A análise da comunidade de cianobactérias presentes em amostras de solos, lagos e canais de degelo de três áreas pertencentes ao domínio da Antártica Marítima revelou 37 taxa do Phylum Cyanophyta, com dominância da Ordem Oscillatoriales. Os organismos de maior distribuição nas amostras são pertencentes aos gêneros Phormidium spp,. Leptolyngbya spp. e Nostoc spp.. Dezessete taxa foram isolados e mantidos em cultivo no Banco de Algas do Laboratório de Ficologia DBV/UFV. Diante da preocupação com o impacto decorrente da contaminação do ambiente antártico com hidrocarbonetos derivados do petróleo sobre as comunidades microbianas, foi testado o efeito de três hidrocarbonetos aromáticos sobre o crescimento de Phormidium autumnale UFV-ANT01. Parâmetros toxicológicos como NOEC, EC20 e EC50 foram estimados. A toxicidade observada sobre o crescimento da cianobactéria testada seguiu a ordem: benzo-α-pireno > antraceno > naftaleno. Concentrações de benzo-α-pireno quatrocentas e cinqüenta vezes menores que as de naftaleno são suficientes para causar a mesma toxicidade sobre Phormidium autumnale UFV-ANT01. As populações desta cianobactéria podem ser uma ferramenta útil no biomonitoramento de áreas contaminadas com hidrocarbonetos. A biorremediação de solos contaminados com hidrocarbonetos pode ser uma alternativa de baixo custo e ecologicamente viável nos ambientes antárticos. A habilidade de três isolados de cianobactérias em degradar [14C]naftaleno, a 15ºC, foi testada. As linhagens Phormidium autumnale UFV-ANT01, Nostoc sp. UFV-ANT23 e Tolypothrix sp. UFV-ANT22 foram testadas em culturas unialgais e em consórcio com bactérias heterotróficas, em comparação a um controle bacteriano e um controle abiótico. Após duzentas horas de exposição ao hidrocarboneto marcado, os tratamentos em consórcio se mostram mais efetivos na degradação do naftaleno que os tratamentos monoclonais e os controles. As taxas de mineralização cumulativas foram de 42%, 39% e 32% para os tratamentos Nostoc em consórcio, Phormidium em consórcio e Tolypotrhix em consórcio, respectivamente, enquanto que o controle bacteriano degradou cerca de 11% do hidrocarboneto adicionado. Os tratamentos monoclonais degradaram entre 2,6 e 4,2% do hidrocarboneto adicionado, sendo considerados menos eficazes que o controle bacteriano, mas não foram estatisticamente diferentes do controle abiótico (1,9%). A cinética de biodegradação observada foi similar à observada por outros autores para modelos expostos a baixas concentrações de hidrocarbonetos, nos quais o ataque microbiano ao contaminante se inicia logo após a aplicação dos tratamentos. As taxas de degradação observadas para os tratamentos em consórcio foram superiores à soma das taxas obtidas para os tratamentos unialgais e o controle bacteriano, sugerindo que as cianobactérias exerçam um papel importante no processo de biodegradação, disponibilizando nutrientes, oxigênio e imobilizando os reais biodegradadores.application/pdfporUniversidade Federal de ViçosaDoutorado em BotânicaUFVBRBotânica estrutural; Ecologia e SistemáticaCianobactériasAntárticaHidrocarbonetosBiodegradaçãoBioindicaçãoCyanobacteriaAntarcticHydrocarbonBiodegradationBioindicationCNPQ::CIENCIAS BIOLOGICAS::BOTANICACaracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetosMorphological and molecular characterization of cyanobacteria from Maritme Antarctic and potential on hydrocarbon biodegradation and bioindication in contaminated environmentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2700640https://locus.ufv.br//bitstream/123456789/367/1/texto%20completo.pdfdb9008a2be7348e338fb6b186d816c2eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain130710https://locus.ufv.br//bitstream/123456789/367/2/texto%20completo.pdf.txt23ffeebd889192ae487206d919bbe628MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3541https://locus.ufv.br//bitstream/123456789/367/3/texto%20completo.pdf.jpg8c0c9107bb12717b8815bca9ef4a39b5MD53123456789/3672016-04-06 23:03:41.926oai:locus.ufv.br:123456789/367Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:03:41LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
dc.title.alternative.eng.fl_str_mv |
Morphological and molecular characterization of cyanobacteria from Maritme Antarctic and potential on hydrocarbon biodegradation and bioindication in contaminated environments |
title |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
spellingShingle |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos Corrêa, Débora Machado Cianobactérias Antártica Hidrocarbonetos Biodegradação Bioindicação Cyanobacteria Antarctic Hydrocarbon Biodegradation Bioindication CNPQ::CIENCIAS BIOLOGICAS::BOTANICA |
title_short |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
title_full |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
title_fullStr |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
title_full_unstemmed |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
title_sort |
Caracterização morfológica e molecular de cianobactérias da Antártica Marítima e potencial para biodegradação e bioindicação em ambientes contaminados por hidrocarbonetos |
author |
Corrêa, Débora Machado |
author_facet |
Corrêa, Débora Machado |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9423333212802361 |
dc.contributor.author.fl_str_mv |
Corrêa, Débora Machado |
dc.contributor.advisor-co1.fl_str_mv |
Albuquerque, Míriam Abreu |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7854974657645438 |
dc.contributor.advisor-co2.fl_str_mv |
Nascimento, Antonio Galvão do |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E8 |
dc.contributor.advisor1.fl_str_mv |
Schaefer, Carlos Ernesto Gonçalves Reynaud |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723204Y8 |
dc.contributor.referee1.fl_str_mv |
Silva, Silvana de Queiroz |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1288764472574843 |
dc.contributor.referee2.fl_str_mv |
Fernandes, Péricles Leonardo |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9781385135118198 |
contributor_str_mv |
Albuquerque, Míriam Abreu Nascimento, Antonio Galvão do Schaefer, Carlos Ernesto Gonçalves Reynaud Silva, Silvana de Queiroz Fernandes, Péricles Leonardo |
dc.subject.por.fl_str_mv |
Cianobactérias Antártica Hidrocarbonetos Biodegradação Bioindicação |
topic |
Cianobactérias Antártica Hidrocarbonetos Biodegradação Bioindicação Cyanobacteria Antarctic Hydrocarbon Biodegradation Bioindication CNPQ::CIENCIAS BIOLOGICAS::BOTANICA |
dc.subject.eng.fl_str_mv |
Cyanobacteria Antarctic Hydrocarbon Biodegradation Bioindication |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA |
description |
The analyses of cyanobacterial communities in soil, lakes and glacial streams samples of three areas within the Maritime Antarctic domain revealed 37 cyanobacterial taxa, with majority belonging to Oscillatoriales Order. The most widespread organisms belonged to genera Phormidium spp,. Leptolyngbya spp. and Nostoc spp.. Seventeen strains were isolated and kept in culture into the Banco de Algas do Laboratório de Ficologia DBV/UFV. Given the concern about the impact of the Antarctic environmental contamination with petroleum hydrocarbons on microbial communities, we tested the effect of three aromatic hydrocarbons on the growth of Phormidium autumnale UFV-ANT01. Toxicological data such as NOEC, EC20 and EC50 were estimated. The toxicity observed on the growth of cyanobacteria tested followed the order: α-benzo-pyrene> anthracene> naphthalene. Concentrations of benzo-α-pyrene four hundred and fifty times smaller than those of naphthalene are sufficient to cause the same toxicity on Phormidium autumnale UFV-ANT01. Populations of cyanobacteria can be a useful tool for the biomonitoring of areas contaminated with hydrocarbons.Bioremediation can be a low cost and ecologic alternative to solve hydrocarbon contamination problems in Antarctic soils. The ability of three cyanobacterial strains in degrading [14C]naphthalene was tested under 15°C. Cyanobacterial lineages Phormidium autumnale UFV-ANT01, Nostoc sp. UFV-ANT23 and Tolypothrix sp. UFV-ANT22 was selected due its important contribution to Antarctic microbial mats. Cyanobateria was investigated in monoclonal cultures and in consortia with heterotrophic bacteria, in comparison with bacterial and abiotic controls. After 200 hours of exposure to labeled hydrocarbons, treatments corresponding to consortia proved to be more effective in [14C]naphthalene than the monoclonal treatments and controls. Cumulative mineralization rates were 42%, 39% and 32%, for Nostoc consortia, Phormidium consortia and Tolypothrix consortia respectively, while bacterial control mineralized 11% of the added hydrocarbon. Monoclonal treatments degraded 2,6 to 4,2% of naphthalene added and were considered less effective than bacterial control, but did not differ from abiotic control (1,9%). Degradation kinetics was similar to those previously reported by other authors for low hydrocarbon concentrations models, in which microbial hydrocarbon attack begins shortly after the contaminant was added to treatments. The rates of hydrocarbon degradation observed to consortia treatments were superior to the sum of individual monoclonal ones and the bacterial control, indicating that cyanobacteria had played a important role in the biodegradation process, providing moisture, nutrients, oxygen and immobilizating the biodegraders microorganisms. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-29 |
dc.date.available.fl_str_mv |
2013-11-28 2015-03-26T12:19:23Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:19:23Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CORRÊA, Débora Machado. Morphological and molecular characterization of cyanobacteria from Maritme Antarctic and potential on hydrocarbon biodegradation and bioindication in contaminated environments. 2012. 74 f. Tese (Doutorado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/367 |
identifier_str_mv |
CORRÊA, Débora Machado. Morphological and molecular characterization of cyanobacteria from Maritme Antarctic and potential on hydrocarbon biodegradation and bioindication in contaminated environments. 2012. 74 f. Tese (Doutorado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2012. |
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http://locus.ufv.br/handle/123456789/367 |
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por |
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por |
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Universidade Federal de Viçosa |
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Doutorado em Botânica |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Botânica estrutural; Ecologia e Sistemática |
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Universidade Federal de Viçosa |
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