Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado

Detalhes bibliográficos
Autor(a) principal: Batista, Fabiane de Mesquita
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5309
Resumo: The heavy oil (10 20ºAPI) is more than half of world oil reserves, there are predictions that in 2025 are the main source of oil in the world. The difficulties in exploration this kind of oil are associated with unfavorable characteristics such as density and viscosity (100 to 10.000 cP). With the need to improve the utilization of reservoir - in view of the relatively low levels of efficiency of traditional techniques - it has expanded the research to develop new techniques for recovery, especially Microbialy Enhanced Oil Recovery (MEOR). In the recovery of oil contained in sand-pack columns, two dimensionless variables are reported as important: the capillary number and ratio of viscosity. As the increases capillary number, the residual oil decreases. This can be achieved by reducing the interfacial tension between the fluid of residual oil. Several authors have reported the tensions interfaciais ultra between biosurfactant and hydrocarbons, including heavy oil. In the present work, tested the hypothesis that the biosurfactant produced on condition of reservoir, by two isolates of Bacillus subtilis from the collection of culture of the Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente (LBBMA / DMB / UFV), are capable to remove heavy oil in sand-pack columns. Initially, the isolates of Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 were evaluated as to the growth and biosurfactant production in response to temperature and salinity. A central composite rotatable design (CCRD) was used, with temperature and salinity being independent variable. The results were analyzed using Response Surface Methodology (RSM) and showed that the those isolates were able of growth and biosurfactant production of under anaerobic conditions. The growth was influenced by temperature and biosurfactant production was influenced by temperature and salinity. After that, the biosurfactant produced by those isolates, alone or mixed, were analyzed as the ability to remove heavy oil trapped in sand-pack columms. The injection of three volumes of porous extract biossurfactante (EB) produced by Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 removed 13.55% and 17.42%, respectively, from residual oil (14 ºAPI). The mixed of extracts from biosurfactant produced by those two isolates showed intermediate values of DMC and recovery of residual oil reported for each. Summarize, the extracts from biosurfactant (EB) produced under anaerobic conditions by Bacillus subtilis LBBMA 155 e Bacillus subtilis subsp. spizizenii LBBM 258 are effective in recovery heavy oil nuclei of the sand-pack columms and have potencial for use Microbialy Enhanced Oil Recovery.
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spelling Batista, Fabiane de Mesquitahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751329H6Nascimento, Antonio Galvão dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E8Silva, Luis Henrique Mendes dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728684Y0Tótola, Marcos Rogériohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727020U4Borges, Arnaldo Chaerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783573Z8Moraes, Célia Alencar dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781007D62015-03-26T13:51:46Z2009-08-282015-03-26T13:51:46Z2008-09-12BATISTA, Fabiane de Mesquita. Biosurfactant production by Bacillus subtilis with enhanced efficiency in heavy oil recovery. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/5309The heavy oil (10 20ºAPI) is more than half of world oil reserves, there are predictions that in 2025 are the main source of oil in the world. The difficulties in exploration this kind of oil are associated with unfavorable characteristics such as density and viscosity (100 to 10.000 cP). With the need to improve the utilization of reservoir - in view of the relatively low levels of efficiency of traditional techniques - it has expanded the research to develop new techniques for recovery, especially Microbialy Enhanced Oil Recovery (MEOR). In the recovery of oil contained in sand-pack columns, two dimensionless variables are reported as important: the capillary number and ratio of viscosity. As the increases capillary number, the residual oil decreases. This can be achieved by reducing the interfacial tension between the fluid of residual oil. Several authors have reported the tensions interfaciais ultra between biosurfactant and hydrocarbons, including heavy oil. In the present work, tested the hypothesis that the biosurfactant produced on condition of reservoir, by two isolates of Bacillus subtilis from the collection of culture of the Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente (LBBMA / DMB / UFV), are capable to remove heavy oil in sand-pack columns. Initially, the isolates of Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 were evaluated as to the growth and biosurfactant production in response to temperature and salinity. A central composite rotatable design (CCRD) was used, with temperature and salinity being independent variable. The results were analyzed using Response Surface Methodology (RSM) and showed that the those isolates were able of growth and biosurfactant production of under anaerobic conditions. The growth was influenced by temperature and biosurfactant production was influenced by temperature and salinity. After that, the biosurfactant produced by those isolates, alone or mixed, were analyzed as the ability to remove heavy oil trapped in sand-pack columms. The injection of three volumes of porous extract biossurfactante (EB) produced by Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 removed 13.55% and 17.42%, respectively, from residual oil (14 ºAPI). The mixed of extracts from biosurfactant produced by those two isolates showed intermediate values of DMC and recovery of residual oil reported for each. Summarize, the extracts from biosurfactant (EB) produced under anaerobic conditions by Bacillus subtilis LBBMA 155 e Bacillus subtilis subsp. spizizenii LBBM 258 are effective in recovery heavy oil nuclei of the sand-pack columms and have potencial for use Microbialy Enhanced Oil Recovery.Os óleos pesados (10-20 ºAPI) constituem mais da metade das reservas mundiais de petróleo, havendo previsões de que em 2025 sejam a principal fonte de petróleo no mundo. As dificuldades na exploração desse tipo de óleo estão associadas a características desfavoráveis, como densidade e viscosidade (100 a 10000 cP). Com a necessidade de melhorar o aproveitamento dos reservatórios tendo em vista os níveis relativamente baixos de eficiência das técnicas tradicionais tem-se ampliado as pesquisas para o desenvolvimento de novas técnicas de recuperação, destacando-se a recuperação avançada de petróleo melhorada por microrganismos (Microbially enhanced oil recovery MEOR). Na recuperação de óleos contidos em meios porosos, duas variáveis adimensionais são relatadas como importantes: o número capilar e a razão de viscosidade. À medida que o número capilar aumenta, o óleo residual decresce. Isso pode ser obtido por meio da redução da tensão interfacial entre o fluido de arraste e o óleo residual. Diversos autores têm reportado a obtenção de tensões interfaciais ultrabaixas entre biossurfactantes e hidrocarbonetos, incluindo o óleo pesado. Neste trabalho, testou-se a hipótese de que os biossurfactantes produzidos, em condição de reservatório, por dois isolados de Bacillus subtilis pertencentes à Coleção de Culturas do Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente (LBBMA/DMB/UFV), são capazes de mobilizar óleo pesado em sistemas porosos de areia. Inicialmente, os isolados de Bacillus subtilis LBBMA 155 e BacilIus subtilis spizizenii LBBMA 258 foram avaliados quanto ao crescimento e produção de biossurfactante em resposta a variações de temperatura e salinidade. Para tanto, foi empregada a Metodologia de Superfície de Resposta (MSR) através do delineamento experimental Composto Central Rotacional (DCCR), e observou-se que esses isolados foram capazes de produzir biossurfactantes em anaerobiose, sendo o crescimento influenciado pela temperatura e a produção de biossurfactante influenciada pela temperatura e pela salinidade. Posteriormente, os biossurfactantes produzidos por esses isolados, sozinhos ou em misturas, foram avaliados quanto à capacidade de mobilização de óleo pesado retido em núcleos porosos de areia. A injeção de três volumes porosos do extrato de biossurfactante (EB) produzidos por Bacillus subtilis LBBMA 155 e Bacillus subtilis subsp. spizizenii LBBMA 258 removeram 13,55% e 17,42%, respectivamente, de petróleo residual (14 ºAPI). A mistura dos extratos de biossurfactantes produzidos por esses dois isolados mostrou valores intermediários de DMC e recuperação de petróleo residual reportados para cada um. Sinteticamente, os extratos de biossurfactantes (EB) produzidos em anaerobiose por B. subtilis LBBMA 155 e B. subtilis subsp. spizizenii LBBMA 258 são efetivos na mobilização de óleo pesado em núcleos porosos de areia e possuem potencial de utilização na recuperação avançada de petróleo melhorada por microrganismos.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesseÓleo pesadoMEORBacillus subtilisAnaerobioseSuperfície de respostaBiossurfactanteNúcleo poroso de areiaHeavy oilMEORBacillus subtilisAnaerobiosisBiosurfactantSurface of responseSand-pack colummsCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADAProdução de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesadoBiosurfactant production by Bacillus subtilis with enhanced efficiency in heavy oil recoveryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL01 - capa_abstract.pdfapplication/pdf113068https://locus.ufv.br//bitstream/123456789/5309/1/01%20-%20capa_abstract.pdfac8d58045328d0ccbe13706fe9c3dedeMD51TEXT01 - capa_abstract.pdf.txt01 - capa_abstract.pdf.txtExtracted texttext/plain7048https://locus.ufv.br//bitstream/123456789/5309/2/01%20-%20capa_abstract.pdf.txt24804fb0527161d4d401c80c1f862aabMD52THUMBNAIL01 - capa_abstract.pdf.jpg01 - capa_abstract.pdf.jpgIM Thumbnailimage/jpeg3548https://locus.ufv.br//bitstream/123456789/5309/3/01%20-%20capa_abstract.pdf.jpg48ae7c2454c80ff814e28d7c5c268340MD53123456789/53092016-04-10 23:17:17.854oai:locus.ufv.br:123456789/5309Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:17:17LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
dc.title.alternative.eng.fl_str_mv Biosurfactant production by Bacillus subtilis with enhanced efficiency in heavy oil recovery
title Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
spellingShingle Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
Batista, Fabiane de Mesquita
Óleo pesado
MEOR
Bacillus subtilis
Anaerobiose
Superfície de resposta
Biossurfactante
Núcleo poroso de areia
Heavy oil
MEOR
Bacillus subtilis
Anaerobiosis
Biosurfactant
Surface of response
Sand-pack columms
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
title_short Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
title_full Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
title_fullStr Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
title_full_unstemmed Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
title_sort Produção de biossurfactante por Bacillus subtilis com elevada eficiência na mobilização de óleo pesado
author Batista, Fabiane de Mesquita
author_facet Batista, Fabiane de Mesquita
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751329H6
dc.contributor.author.fl_str_mv Batista, Fabiane de Mesquita
dc.contributor.advisor-co1.fl_str_mv Nascimento, Antonio Galvão do
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E8
dc.contributor.advisor-co2.fl_str_mv Silva, Luis Henrique Mendes da
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728684Y0
dc.contributor.advisor1.fl_str_mv Tótola, Marcos Rogério
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727020U4
dc.contributor.referee1.fl_str_mv Borges, Arnaldo Chaer
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783573Z8
dc.contributor.referee2.fl_str_mv Moraes, Célia Alencar de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781007D6
contributor_str_mv Nascimento, Antonio Galvão do
Silva, Luis Henrique Mendes da
Tótola, Marcos Rogério
Borges, Arnaldo Chaer
Moraes, Célia Alencar de
dc.subject.por.fl_str_mv Óleo pesado
MEOR
Bacillus subtilis
Anaerobiose
Superfície de resposta
Biossurfactante
Núcleo poroso de areia
topic Óleo pesado
MEOR
Bacillus subtilis
Anaerobiose
Superfície de resposta
Biossurfactante
Núcleo poroso de areia
Heavy oil
MEOR
Bacillus subtilis
Anaerobiosis
Biosurfactant
Surface of response
Sand-pack columms
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
dc.subject.eng.fl_str_mv Heavy oil
MEOR
Bacillus subtilis
Anaerobiosis
Biosurfactant
Surface of response
Sand-pack columms
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
description The heavy oil (10 20ºAPI) is more than half of world oil reserves, there are predictions that in 2025 are the main source of oil in the world. The difficulties in exploration this kind of oil are associated with unfavorable characteristics such as density and viscosity (100 to 10.000 cP). With the need to improve the utilization of reservoir - in view of the relatively low levels of efficiency of traditional techniques - it has expanded the research to develop new techniques for recovery, especially Microbialy Enhanced Oil Recovery (MEOR). In the recovery of oil contained in sand-pack columns, two dimensionless variables are reported as important: the capillary number and ratio of viscosity. As the increases capillary number, the residual oil decreases. This can be achieved by reducing the interfacial tension between the fluid of residual oil. Several authors have reported the tensions interfaciais ultra between biosurfactant and hydrocarbons, including heavy oil. In the present work, tested the hypothesis that the biosurfactant produced on condition of reservoir, by two isolates of Bacillus subtilis from the collection of culture of the Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente (LBBMA / DMB / UFV), are capable to remove heavy oil in sand-pack columns. Initially, the isolates of Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 were evaluated as to the growth and biosurfactant production in response to temperature and salinity. A central composite rotatable design (CCRD) was used, with temperature and salinity being independent variable. The results were analyzed using Response Surface Methodology (RSM) and showed that the those isolates were able of growth and biosurfactant production of under anaerobic conditions. The growth was influenced by temperature and biosurfactant production was influenced by temperature and salinity. After that, the biosurfactant produced by those isolates, alone or mixed, were analyzed as the ability to remove heavy oil trapped in sand-pack columms. The injection of three volumes of porous extract biossurfactante (EB) produced by Bacillus subtilis LBBMA 155 and Bacillus subtilis subsp. spizizenii LBBMA 258 removed 13.55% and 17.42%, respectively, from residual oil (14 ºAPI). The mixed of extracts from biosurfactant produced by those two isolates showed intermediate values of DMC and recovery of residual oil reported for each. Summarize, the extracts from biosurfactant (EB) produced under anaerobic conditions by Bacillus subtilis LBBMA 155 e Bacillus subtilis subsp. spizizenii LBBM 258 are effective in recovery heavy oil nuclei of the sand-pack columms and have potencial for use Microbialy Enhanced Oil Recovery.
publishDate 2008
dc.date.issued.fl_str_mv 2008-09-12
dc.date.available.fl_str_mv 2009-08-28
2015-03-26T13:51:46Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:51:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BATISTA, Fabiane de Mesquita. Biosurfactant production by Bacillus subtilis with enhanced efficiency in heavy oil recovery. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5309
identifier_str_mv BATISTA, Fabiane de Mesquita. Biosurfactant production by Bacillus subtilis with enhanced efficiency in heavy oil recovery. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
url http://locus.ufv.br/handle/123456789/5309
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dc.publisher.program.fl_str_mv Mestrado em Microbiologia Agrícola
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
publisher.none.fl_str_mv Universidade Federal de Viçosa
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