Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma

• Autor(a) principal: Souza, Maria Verônica de
• Data de Publicação: 2015
• Outros Autores: Silva, Mariana Brettas, Pinto, José de Oliveira, Crepaldi, Júlio, Lima, Marianna Barros de Souza, Lopes, Gabriela Francine Martins, Santos, Hélio Batista dos, Ribeiro, Rosy Iara Maciel de Azambuja, Thomé, Ralph Gruppi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1155/2015/893485; http://www.locus.ufv.br/handle/123456789/12608
• topic: Immunohistochemical; Collagens; Horses; Leukocyte-Poor; Platelet-Rich Plasma

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.