Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma

Detalhes bibliográficos
Autor(a) principal: Souza, Maria Verônica de
Data de Publicação: 2015
Outros Autores: Silva, Mariana Brettas, Pinto, José de Oliveira, Crepaldi, Júlio, Lima, Marianna Barros de Souza, Lopes, Gabriela Francine Martins, Santos, Hélio Batista dos, Ribeiro, Rosy Iara Maciel de Azambuja, Thomé, Ralph Gruppi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1155/2015/893485
http://www.locus.ufv.br/handle/123456789/12608
Resumo: This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.
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spelling Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasmaImmunohistochemicalCollagensHorsesLeukocyte-PoorPlatelet-Rich PlasmaThis study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.BioMed Research International2017-10-31T12:49:26Z2017-10-31T12:49:26Z2015-05-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf2314-6133http://dx.doi.org/10.1155/2015/893485http://www.locus.ufv.br/handle/123456789/12608engVolume 2015, Article ID 893485, May 2015Souza, Maria Verônica deSilva, Mariana BrettasPinto, José de OliveiraCrepaldi, JúlioLima, Marianna Barros de SouzaLopes, Gabriela Francine MartinsSantos, Hélio Batista dosRibeiro, Rosy Iara Maciel de AzambujaThomé, Ralph Gruppiinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:35:51Zoai:locus.ufv.br:123456789/12608Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:35:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
title Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
spellingShingle Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
Souza, Maria Verônica de
Immunohistochemical
Collagens
Horses
Leukocyte-Poor
Platelet-Rich Plasma
title_short Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
title_full Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
title_fullStr Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
title_full_unstemmed Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
title_sort Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
author Souza, Maria Verônica de
author_facet Souza, Maria Verônica de
Silva, Mariana Brettas
Pinto, José de Oliveira
Crepaldi, Júlio
Lima, Marianna Barros de Souza
Lopes, Gabriela Francine Martins
Santos, Hélio Batista dos
Ribeiro, Rosy Iara Maciel de Azambuja
Thomé, Ralph Gruppi
author_role author
author2 Silva, Mariana Brettas
Pinto, José de Oliveira
Crepaldi, Júlio
Lima, Marianna Barros de Souza
Lopes, Gabriela Francine Martins
Santos, Hélio Batista dos
Ribeiro, Rosy Iara Maciel de Azambuja
Thomé, Ralph Gruppi
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Maria Verônica de
Silva, Mariana Brettas
Pinto, José de Oliveira
Crepaldi, Júlio
Lima, Marianna Barros de Souza
Lopes, Gabriela Francine Martins
Santos, Hélio Batista dos
Ribeiro, Rosy Iara Maciel de Azambuja
Thomé, Ralph Gruppi
dc.subject.por.fl_str_mv Immunohistochemical
Collagens
Horses
Leukocyte-Poor
Platelet-Rich Plasma
topic Immunohistochemical
Collagens
Horses
Leukocyte-Poor
Platelet-Rich Plasma
description This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-12
2017-10-31T12:49:26Z
2017-10-31T12:49:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 2314-6133
http://dx.doi.org/10.1155/2015/893485
http://www.locus.ufv.br/handle/123456789/12608
identifier_str_mv 2314-6133
url http://dx.doi.org/10.1155/2015/893485
http://www.locus.ufv.br/handle/123456789/12608
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Volume 2015, Article ID 893485, May 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Research International
publisher.none.fl_str_mv BioMed Research International
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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