Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1155/2015/893485 http://www.locus.ufv.br/handle/123456789/12608 |
Resumo: | This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished. |
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LOCUS Repositório Institucional da UFV |
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2145 |
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Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasmaImmunohistochemicalCollagensHorsesLeukocyte-PoorPlatelet-Rich PlasmaThis study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.BioMed Research International2017-10-31T12:49:26Z2017-10-31T12:49:26Z2015-05-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf2314-6133http://dx.doi.org/10.1155/2015/893485http://www.locus.ufv.br/handle/123456789/12608engVolume 2015, Article ID 893485, May 2015Souza, Maria Verônica deSilva, Mariana BrettasPinto, José de OliveiraCrepaldi, JúlioLima, Marianna Barros de SouzaLopes, Gabriela Francine MartinsSantos, Hélio Batista dosRibeiro, Rosy Iara Maciel de AzambujaThomé, Ralph Gruppiinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:35:51Zoai:locus.ufv.br:123456789/12608Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:35:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
title |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
spellingShingle |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma Souza, Maria Verônica de Immunohistochemical Collagens Horses Leukocyte-Poor Platelet-Rich Plasma |
title_short |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
title_full |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
title_fullStr |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
title_full_unstemmed |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
title_sort |
Immunohistochemical expression of collagens in the skin of horses treated with leukocyte-poor platelet-rich plasma |
author |
Souza, Maria Verônica de |
author_facet |
Souza, Maria Verônica de Silva, Mariana Brettas Pinto, José de Oliveira Crepaldi, Júlio Lima, Marianna Barros de Souza Lopes, Gabriela Francine Martins Santos, Hélio Batista dos Ribeiro, Rosy Iara Maciel de Azambuja Thomé, Ralph Gruppi |
author_role |
author |
author2 |
Silva, Mariana Brettas Pinto, José de Oliveira Crepaldi, Júlio Lima, Marianna Barros de Souza Lopes, Gabriela Francine Martins Santos, Hélio Batista dos Ribeiro, Rosy Iara Maciel de Azambuja Thomé, Ralph Gruppi |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Maria Verônica de Silva, Mariana Brettas Pinto, José de Oliveira Crepaldi, Júlio Lima, Marianna Barros de Souza Lopes, Gabriela Francine Martins Santos, Hélio Batista dos Ribeiro, Rosy Iara Maciel de Azambuja Thomé, Ralph Gruppi |
dc.subject.por.fl_str_mv |
Immunohistochemical Collagens Horses Leukocyte-Poor Platelet-Rich Plasma |
topic |
Immunohistochemical Collagens Horses Leukocyte-Poor Platelet-Rich Plasma |
description |
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-12 2017-10-31T12:49:26Z 2017-10-31T12:49:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
2314-6133 http://dx.doi.org/10.1155/2015/893485 http://www.locus.ufv.br/handle/123456789/12608 |
identifier_str_mv |
2314-6133 |
url |
http://dx.doi.org/10.1155/2015/893485 http://www.locus.ufv.br/handle/123456789/12608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Volume 2015, Article ID 893485, May 2015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Research International |
publisher.none.fl_str_mv |
BioMed Research International |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817560027960967168 |