Efeitos do cádmio, chumbo e zinco em epidídimo de ratos Wistar

• Autor(a) principal: Ribeiro, Susana Puga
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2278
• topic: Toxicologia; Metal pesado; Reprodução; Morfometria; Toxicology; Heavy metal; Reproduction; Morphometry; CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA

There is considerable interest in the role of occupational and environmental exposure to toxic pollutants such as heavy metals, because we understand that this is one of the causes of reduced sperm concentration in man. Therefore, the study of the influence of heavy metals in males has focused mainly on the reproductive system, site development, differentiation, maturation and capacitation of germ cells. This study investigated the effects of increasing doses of heavy metals, cadmium (Cd), lead (Pb) and zinc (Zn) in the epididymis of rats. The work was divided into three experiments were used adult rats of reproductive age, placed in individual cages in a controlled environment. To assess the effect of Cd on the epididymis, 24 animals were divided into 4 experimental groups and one control group and three groups that received respectively 1.1, 1.4 and 1.8 mg / kg intraperitoneally cadmium chloride, a single dose. For the evaluation of the effects of Pb in the epididymis 25 animals were used divided into 5 experimental groups, one control and four receiving lead acetate by gavage at concentrations of 16, 32, 64, 128 mg / kg / day, respectively . In the evaluation of the effects of Zn 28 animals were divided into 4 groups with zinc sulfate administered to three groups in the drinking water at concentrations of 5, 10 and 20 mg / day. Animals receiving Cd and Zn were euthanized after 56 days of treatment while animals subjected to Pb were euthanized at 30 days. The following analyzes were performed in all epididymides collected: diameter tubular (DT), lumen diameter (LD), height of the epithelium (EP) and the volumetric proportions (%) of the epithelium (E), connective tissue (CT), with lumen sperm (LCE) and lumen without sperm (LSE), the initial segment, head and tail of the epididymis. Cadmium caused an increase in epithelial height of the head of the epididymis and increased luminal diameter tubular tail. Moreover proportional parameters were reduced in proportion to the epithelium of the head and tail of the epididymis. Even the head of the epididymis, the proportion of sperm with lumen was reduced while the proportion of the lumen without sperm increased both the initial segment as the head and tail of the epididymis. The lead, in all the treatments, at the tail region, significantly reduced the diameter of the lumen and epididymal tubule, while the head caused an increase in epithelial height and reduction in its amount. The effects of zinc, significant reductions were observed in epithelial height, both in the initial segment, head and tail of the epididymis and the diameters of the tubule lumen and increased in the caudal portion. Thus, we believe that all three evaluated metals caused morphological changes of the organ in the three regions studied, the most often toxic showing the epididymis and that the cadmium was more toxic to the reproductive process, due to the increased lumen without sperm causing in all epididymal evaluated regions, thus causing loss in reproductive capacity of these animals.