Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro

Detalhes bibliográficos
Autor(a) principal: Fonseca, Juliana de Oliveira
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/3964
Resumo: Plants protect themselves against herbivore attacks by a combination of constitutive and induced defenses, which reduce the performance of herbivores and decrease the spread of damage. Plants under attack by herbivores release a variety of organic volatile compounds, which are involved in the attraction of natural enemies of herbivores. The natural enemies such as predatory mites and coccinellids, for example, are able to exploit these volatiles to locate their prey, using them as chemical signals for the presence of food. Thus, behavioral studies are important for understanding the interactions that occur between organisms in a food web. As well as the knowledge of the chemical compounds that mediate the location of plants infested with herbivores by natural enemies, in order to have a better understanding of insect-plant interactions mediating the third trophic level. In this study, we investigated the responses of the predators Phytoseiulus longipes, Phytoseiulus macropilis and Cycloneda sanguinea to volatiles from tomato plants infested or not infested with the phytophagous mites Tetranychus evansi or Tetranychus urticae. The main objective was to verify whether these predators are able to discriminate between sources of odors produced by the plant-herbivore complex. We also identified the volatile compounds emitted by plants infested with T. evansi, T. urticae and clean tomato plants. To access these results were used techniques of olfactometry for behavioral tests and GC-MS to identify the compounds. The results show that the tested predators are able to discriminate between volatile tomato plants infested with T. evansi or T. urticae and volatile of clean plants, showing that the plant-herbivore complex releases odors attractive to these natural enemies. But when given the choice between plants infested with T. evansi and plants infested with T. urticae, the predators are not able to discriminate between the two sources of odors produced. The identification of volatile compounds was verified that when comparing clean plants and plants infested with T. evansi or T. urticae was found and identified more volatile compounds in infested plants. When we compare plants infested with T. evansi and plants infested with T. urticae, the profile of the volatile compounds were very similar in both. As for the quantitative analysis of compounds, we found no significant difference between the concentrations of each compound emitted by plants infested with T. evansi and T. urticae. The results obtained through the identification and quantification of volatile compounds may explain the responses obtained in the olfactometer experiments.
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spelling Fonseca, Juliana de Oliveirahttp://lattes.cnpq.br/3546103311236262Janssen, Arnoldus Rudolf Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706455Y2Venzon, Madelainehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795615T1Pallini Filho, ângelohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798707A3Lima, Eraldo Rodrigues dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783762J5Sarmento, Renato de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762029E82015-03-26T13:30:44Z2013-04-122015-03-26T13:30:44Z2012-07-30FONSECA, Juliana de Oliveira. Identification of volatile compounds produced in the predator-prey-tomato system. 2012. 58 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/3964Plants protect themselves against herbivore attacks by a combination of constitutive and induced defenses, which reduce the performance of herbivores and decrease the spread of damage. Plants under attack by herbivores release a variety of organic volatile compounds, which are involved in the attraction of natural enemies of herbivores. The natural enemies such as predatory mites and coccinellids, for example, are able to exploit these volatiles to locate their prey, using them as chemical signals for the presence of food. Thus, behavioral studies are important for understanding the interactions that occur between organisms in a food web. As well as the knowledge of the chemical compounds that mediate the location of plants infested with herbivores by natural enemies, in order to have a better understanding of insect-plant interactions mediating the third trophic level. In this study, we investigated the responses of the predators Phytoseiulus longipes, Phytoseiulus macropilis and Cycloneda sanguinea to volatiles from tomato plants infested or not infested with the phytophagous mites Tetranychus evansi or Tetranychus urticae. The main objective was to verify whether these predators are able to discriminate between sources of odors produced by the plant-herbivore complex. We also identified the volatile compounds emitted by plants infested with T. evansi, T. urticae and clean tomato plants. To access these results were used techniques of olfactometry for behavioral tests and GC-MS to identify the compounds. The results show that the tested predators are able to discriminate between volatile tomato plants infested with T. evansi or T. urticae and volatile of clean plants, showing that the plant-herbivore complex releases odors attractive to these natural enemies. But when given the choice between plants infested with T. evansi and plants infested with T. urticae, the predators are not able to discriminate between the two sources of odors produced. The identification of volatile compounds was verified that when comparing clean plants and plants infested with T. evansi or T. urticae was found and identified more volatile compounds in infested plants. When we compare plants infested with T. evansi and plants infested with T. urticae, the profile of the volatile compounds were very similar in both. As for the quantitative analysis of compounds, we found no significant difference between the concentrations of each compound emitted by plants infested with T. evansi and T. urticae. The results obtained through the identification and quantification of volatile compounds may explain the responses obtained in the olfactometer experiments.As plantas se protegem contra o ataque de herbívoros através da combinação de defesas constitutivas e/ou induzidas que reduzem o desempenho dos herbívoros e, consequentemente, interrompem a propagação do dano. Plantas sadias ou sob o ataque de herbívoros liberam uma variedade de compostos orgânicos voláteis, os quais estão envolvidos na atração dos inimigos naturais desses herbívoros. Os inimigos naturais como ácaros predadores e coccinelídeos, por exemplo, são capazes de explorar esses voláteis para localizar suas presas, usando-os como sinais químicos da presença do alimento. Dessa forma, estudos de comportamento são importantes para o entendimento das interações que ocorrem entre os organismos em uma teia alimentar. Assim como o conhecimento dos compostos químicos que mediam a localização de plantas infestadas com herbívoros pelos inimigos naturais, para que se tenha uma melhor compreensão da interação inseto-planta influenciando o terceiro nível trófico. Diante disso, este trabalho objetivou investigar as respostas dos ácaros predadores Phytoseiulus longipes e Phytoseiulus macropilis e de joaninhas da espécie Cycloneda sanguinea aos voláteis de plantas de tomate infestadas ou não com Tetranychus evansi e Tetranychus urticae. O principal objetivo foi verificar se estes predadores são capazes de discriminar entre as fontes de odores produzidas pelo complexo planta-herbívoro. Nós também identificamos os compostos voláteis emitidos por plantas infestadas com o ácaro-vermelho T. evansi e com o ácaro rajado T. urticae e de plantas de tomate limpas. Para acessar esses resultados foram utilizadas as técnicas de olfatometria para os testes comportamentais e o GC-MS para a identificação dos compostos. Os resultados mostram que os predadores testados são capazes de discriminar entre voláteis de plantas de tomate infestadas com T. evansi ou T. urticae e voláteis de plantas limpas. Isso mostra que o complexo planta-herbívoro libera odores atraentes a estes inimigos naturais. Porém, quando dada à escolha entre plantas infestadas com T. evansi e plantas infestadas com T. urticae, os predadores não são capazes de discriminar entre as duas fontes de odores produzidas. Quanto à identificação dos compostos foi verificado que ao compararmos plantas limpas e plantas infestadas, seja com o ácaro-vermelho ou com o ácaro rajado, foram encontrados e identificados uma quantidade bastante superior de compostos voláteis nas plantas infestadas. Já quando comparamos plantas infestadas com T. evansi e plantas infestadas com T. urticae, o perfil de compostos voláteis encontrado em ambas foi bastante similar. Quanto à análise quantitativa dos compostos, observamos que não existe diferença significativa entre as concentrações de cada composto emitidos por plantas infestadas com T. evansi e T. urticae. Os resultados obtidos através da identificação e quantificação dos compostos voláteis podem explicar as respostas obtidas nos experimentos de olfatômetro.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em EntomologiaUFVBRCiência entomológica; Tecnologia entomológicaInimigos naturaisTetranychus evansiTetranychus urticaetomateNatural enemiesTetranychus evansiTetranychus urticaeTomato plantsCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::ENTOMOLOGIA AGRICOLAIdentificação de compostos voláteis produzidos no sistema predador-presa-tomateiroIdentification of volatile compounds produced in the predator-prey-tomato systeminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf301316https://locus.ufv.br//bitstream/123456789/3964/1/texto%20completo.pdf99cd4e8684305bf084ac2f725463bae5MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain89554https://locus.ufv.br//bitstream/123456789/3964/2/texto%20completo.pdf.txtb4a25e3277dc777ac3aa5936d41a9007MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3479https://locus.ufv.br//bitstream/123456789/3964/3/texto%20completo.pdf.jpgc7cf850d9f8136ae65b64b7753a765aeMD53123456789/39642016-04-09 23:12:37.13oai:locus.ufv.br:123456789/3964Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-10T02:12:37LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
dc.title.alternative.eng.fl_str_mv Identification of volatile compounds produced in the predator-prey-tomato system
title Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
spellingShingle Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
Fonseca, Juliana de Oliveira
Inimigos naturais
Tetranychus evansi
Tetranychus urticae
tomate
Natural enemies
Tetranychus evansi
Tetranychus urticae
Tomato plants
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::ENTOMOLOGIA AGRICOLA
title_short Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
title_full Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
title_fullStr Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
title_full_unstemmed Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
title_sort Identificação de compostos voláteis produzidos no sistema predador-presa-tomateiro
author Fonseca, Juliana de Oliveira
author_facet Fonseca, Juliana de Oliveira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/3546103311236262
dc.contributor.author.fl_str_mv Fonseca, Juliana de Oliveira
dc.contributor.advisor-co1.fl_str_mv Janssen, Arnoldus Rudolf Maria
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706455Y2
dc.contributor.advisor-co2.fl_str_mv Venzon, Madelaine
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795615T1
dc.contributor.advisor1.fl_str_mv Pallini Filho, ângelo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798707A3
dc.contributor.referee1.fl_str_mv Lima, Eraldo Rodrigues de
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783762J5
dc.contributor.referee2.fl_str_mv Sarmento, Renato de Almeida
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762029E8
contributor_str_mv Janssen, Arnoldus Rudolf Maria
Venzon, Madelaine
Pallini Filho, ângelo
Lima, Eraldo Rodrigues de
Sarmento, Renato de Almeida
dc.subject.por.fl_str_mv Inimigos naturais
Tetranychus evansi
Tetranychus urticae
tomate
topic Inimigos naturais
Tetranychus evansi
Tetranychus urticae
tomate
Natural enemies
Tetranychus evansi
Tetranychus urticae
Tomato plants
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::ENTOMOLOGIA AGRICOLA
dc.subject.eng.fl_str_mv Natural enemies
Tetranychus evansi
Tetranychus urticae
Tomato plants
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::ENTOMOLOGIA AGRICOLA
description Plants protect themselves against herbivore attacks by a combination of constitutive and induced defenses, which reduce the performance of herbivores and decrease the spread of damage. Plants under attack by herbivores release a variety of organic volatile compounds, which are involved in the attraction of natural enemies of herbivores. The natural enemies such as predatory mites and coccinellids, for example, are able to exploit these volatiles to locate their prey, using them as chemical signals for the presence of food. Thus, behavioral studies are important for understanding the interactions that occur between organisms in a food web. As well as the knowledge of the chemical compounds that mediate the location of plants infested with herbivores by natural enemies, in order to have a better understanding of insect-plant interactions mediating the third trophic level. In this study, we investigated the responses of the predators Phytoseiulus longipes, Phytoseiulus macropilis and Cycloneda sanguinea to volatiles from tomato plants infested or not infested with the phytophagous mites Tetranychus evansi or Tetranychus urticae. The main objective was to verify whether these predators are able to discriminate between sources of odors produced by the plant-herbivore complex. We also identified the volatile compounds emitted by plants infested with T. evansi, T. urticae and clean tomato plants. To access these results were used techniques of olfactometry for behavioral tests and GC-MS to identify the compounds. The results show that the tested predators are able to discriminate between volatile tomato plants infested with T. evansi or T. urticae and volatile of clean plants, showing that the plant-herbivore complex releases odors attractive to these natural enemies. But when given the choice between plants infested with T. evansi and plants infested with T. urticae, the predators are not able to discriminate between the two sources of odors produced. The identification of volatile compounds was verified that when comparing clean plants and plants infested with T. evansi or T. urticae was found and identified more volatile compounds in infested plants. When we compare plants infested with T. evansi and plants infested with T. urticae, the profile of the volatile compounds were very similar in both. As for the quantitative analysis of compounds, we found no significant difference between the concentrations of each compound emitted by plants infested with T. evansi and T. urticae. The results obtained through the identification and quantification of volatile compounds may explain the responses obtained in the olfactometer experiments.
publishDate 2012
dc.date.issued.fl_str_mv 2012-07-30
dc.date.available.fl_str_mv 2013-04-12
2015-03-26T13:30:44Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:30:44Z
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dc.identifier.citation.fl_str_mv FONSECA, Juliana de Oliveira. Identification of volatile compounds produced in the predator-prey-tomato system. 2012. 58 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2012.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/3964
identifier_str_mv FONSECA, Juliana de Oliveira. Identification of volatile compounds produced in the predator-prey-tomato system. 2012. 58 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2012.
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