Efeitos de alendronato de sódio, sinvastatina, crisina e tintura de Camellia sinensis na osteoporose induzida por dexametasona em ratas

• Autor(a) principal: Sequetto, Priscila Lima
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2405
• topic: Osteoporose; Flavonóides; Dexametasona; Camellia sinensis; Osteoporosis; Flavononoid; Dexametasona; Camellia sinensis; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR

The present work had the objective to evaluate the influence of the sodium alendronate, simvastatin, flavononoid chrysin and the Camellia sinensis ticture used separately and in associated, in osteoporose induced for the administration of dexametasone (dose of 7mg/kg of weight), in rats of the Wistar ancestry (Rattus norvegicus albinus). The used dosages were: sodium alendronate dose 1 (0,20 mg), dose 2 (0,40 mg) and dose 3 (0,60 mg); simvastatin dose 1 (0,40 mg), dose 2 (0,70 mg) and dose 3 (1,0 mg); flavonoid dose 1 (35 mg), dose 2 (60 mg) and dose 3 (90 mg) and phytoterapic dose (0,01 mg), dose 2 (0,02 mg) and dose 3 (0,03 mg), treated once a day orally. After the osteoporosis induction period, the treatments were initiated. The animals were distributed in fourteen groups: G1 = Comercial ration (Control); G2 = Comercial ration + Dexametasone (Control with osteoporose); G3 = Comercial ration + Dexametasone + Biphosphonate dose 1; G4 = Comercial ration + Dexametasone + Biphosphonate dose 2; G5 = Comercial ration + Dexametasone + Biphosphonate dose 3; G6 = Comercial ration + Dexametasone + Biphosphonate dose 1 + simvastatin dose 1; G7 = Comercial ration + Dexametasone + Biphosphonate dose 2 + simvastatin dose 2; G8 = Comercial ration + Dexametasone + Biphosphonate dose 3 + simvastatin dose 3; G9 = Comercial ration + Dexametasone + Flavonoid dose 1; G10 = Comercial ration + Dexametasone + Flavonoid dose 2; G11 = Comercial ration + Dexametasone + Flavonoid dose 3; G12 = Comercial ration + Dexametasone + Phytoterapic dose 1; G13 = Comercial ration + Dexametasone + Phytoterapic dose 2; G14 = Comercial ration + Dexametasone + Phytoterapic dose 3. After 30 days from the start of the treatment, seven animals of each group had been euthanasieted by anesthetical overdosage of 180 mg/Kg de ketamin e 10 mg/Kg de xilasin, route to intraperitoneal. It has been collected of each animal, 5,0 mL of blood by cardiac punction, for the hematological analyses and biochemist quantification of the serum levels of calcium, phosphorus, glucose, fosfatase alkaline total, urea, creatinina, proteins, total cholesterol, tryacylglicerols, gamma GT and albumen. The results of the biochemists analyses of calcium, phosphorus, glucose, fosfatase alkaline total, urea, creatinina, proteins, total cholesterol, tryacylglicerols, gamma GT and albumen had not shown significant differences among the treated groups, being significant only in relation to the control animals (with osteoporosis). Also, it had not have significant differences in the dosages of the hematological parameters between the control group and the control group with osteoporosis, as well as, among them and the treated groups. The electronic microphotographs shows that there was an increase of the porosity in the bones of the control animals (with osteoporosis) and a reduction of these pores in the treated animals. This could also be observed through the evaluation of the bone histopatology, that it showed a recovery in the trabecular area on the treated animals when compared to the control with osteoporosis.