Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa

Detalhes bibliográficos
Autor(a) principal: Santana, Mateus Ferreira
Data de Publicação: 2012
Outros Autores: Araújo, Elza Fernandes de, Queiroz, Marisa Vieira de, Souza, Jorge Teodoro de, Mizubuti, Eduardo Seiti Gomide
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/s10658-012-0031-4
http://www.locus.ufv.br/handle/123456789/22902
Resumo: Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.
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spelling Santana, Mateus FerreiraAraújo, Elza Fernandes deQueiroz, Marisa Vieira deSouza, Jorge Teodoro deMizubuti, Eduardo Seiti Gomide2019-01-03T15:50:57Z2019-01-03T15:50:57Z2012-111573-8469https://doi.org/10.1007/s10658-012-0031-4http://www.locus.ufv.br/handle/123456789/22902Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.engEuropean Journal of Plant PathologyVolume 134, Issue 3, Pages 497– 507, November 2012KNPV 2012info:eu-repo/semantics/openAccessRetrotransposonMoniliophthora perniciosaGenetic variabilityIRAPREMAPDevelopment of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf385061https://locus.ufv.br//bitstream/123456789/22902/1/artigo.pdf95be5973a45dcb5a7826ae2e8cc84442MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22902/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/229022019-01-03 14:06:07.686oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-01-03T17:06:07LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
title Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
spellingShingle Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
Santana, Mateus Ferreira
Retrotransposon
Moniliophthora perniciosa
Genetic variability
IRAP
REMAP
title_short Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
title_full Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
title_fullStr Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
title_full_unstemmed Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
title_sort Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
author Santana, Mateus Ferreira
author_facet Santana, Mateus Ferreira
Araújo, Elza Fernandes de
Queiroz, Marisa Vieira de
Souza, Jorge Teodoro de
Mizubuti, Eduardo Seiti Gomide
author_role author
author2 Araújo, Elza Fernandes de
Queiroz, Marisa Vieira de
Souza, Jorge Teodoro de
Mizubuti, Eduardo Seiti Gomide
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santana, Mateus Ferreira
Araújo, Elza Fernandes de
Queiroz, Marisa Vieira de
Souza, Jorge Teodoro de
Mizubuti, Eduardo Seiti Gomide
dc.subject.pt-BR.fl_str_mv Retrotransposon
Moniliophthora perniciosa
Genetic variability
IRAP
REMAP
topic Retrotransposon
Moniliophthora perniciosa
Genetic variability
IRAP
REMAP
description Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.
publishDate 2012
dc.date.issued.fl_str_mv 2012-11
dc.date.accessioned.fl_str_mv 2019-01-03T15:50:57Z
dc.date.available.fl_str_mv 2019-01-03T15:50:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1007/s10658-012-0031-4
http://www.locus.ufv.br/handle/123456789/22902
dc.identifier.issn.none.fl_str_mv 1573-8469
identifier_str_mv 1573-8469
url https://doi.org/10.1007/s10658-012-0031-4
http://www.locus.ufv.br/handle/123456789/22902
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 134, Issue 3, Pages 497– 507, November 2012
dc.rights.driver.fl_str_mv KNPV 2012
info:eu-repo/semantics/openAccess
rights_invalid_str_mv KNPV 2012
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv European Journal of Plant Pathology
publisher.none.fl_str_mv European Journal of Plant Pathology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/22902/1/artigo.pdf
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repository.mail.fl_str_mv fabiojreis@ufv.br
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