Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer

Detalhes bibliográficos
Autor(a) principal: Passos, Flávia Maria Lopes
Data de Publicação: 2001
Outros Autores: Passos, Frederico José Vieira, Silva, Daison Olzany, Piccoli-Valle, Roberta Hilsdorf
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1517-83822001000200013
https://locus.ufv.br//handle/123456789/26916
Resumo: Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.
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spelling Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducerFermentationPectin lyasePenicillium griseoroseumPectinasesFermentaçãoPectina liasePenicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.Penicillium griseoroseum foi cultivado em biorreatores em meio mineral suplementado com extrato de levedura e sacarose. As influências das concentrações do inóculo e da fonte de carbono, da aeração e do pH do meio de cultivo sobre a produção de pectina liase (PL), bem como a capacidade de P. griseoroseum em produzir PL quando cultivado em caldo de cana diluído foram avaliadas. A concentração do inóculo não influenciou significativamente a produção de PL. O cultivo do fungo em biorreatores não aerados favoreceu a produção da PL em detrimento aos biorreatores com injeção de ar. Maior produção de PL foi obtida com o cultivo de P. griseoroseum em meio com pH 6,3 – 7,2, adicionado de 60 mM de sacarose. Quando cultivado em caldo de cana diluído, 25% (v/v), sem suplementação com extrato de levedura, a atividade máxima de PL alcançada foi igual as das condições citadas acima.Brazilian Journal of Microbiology2019-09-10T11:56:20Z2019-09-10T11:56:20Z2001-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1678-4405http://dx.doi.org/10.1590/S1517-83822001000200013https://locus.ufv.br//handle/123456789/26916engv. 32, n. 02, p. 135- 140, abr./ jun. 2001Passos, Flávia Maria LopesPassos, Frederico José VieiraSilva, Daison OlzanyPiccoli-Valle, Roberta Hilsdorfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:13:09Zoai:locus.ufv.br:123456789/26916Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:13:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
title Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
spellingShingle Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
Passos, Flávia Maria Lopes
Fermentation
Pectin lyase
Penicillium griseoroseum
Pectinases
Fermentação
Pectina liase
title_short Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
title_full Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
title_fullStr Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
title_full_unstemmed Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
title_sort Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer
author Passos, Flávia Maria Lopes
author_facet Passos, Flávia Maria Lopes
Passos, Frederico José Vieira
Silva, Daison Olzany
Piccoli-Valle, Roberta Hilsdorf
author_role author
author2 Passos, Frederico José Vieira
Silva, Daison Olzany
Piccoli-Valle, Roberta Hilsdorf
author2_role author
author
author
dc.contributor.author.fl_str_mv Passos, Flávia Maria Lopes
Passos, Frederico José Vieira
Silva, Daison Olzany
Piccoli-Valle, Roberta Hilsdorf
dc.subject.por.fl_str_mv Fermentation
Pectin lyase
Penicillium griseoroseum
Pectinases
Fermentação
Pectina liase
topic Fermentation
Pectin lyase
Penicillium griseoroseum
Pectinases
Fermentação
Pectina liase
description Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.
publishDate 2001
dc.date.none.fl_str_mv 2001-04
2019-09-10T11:56:20Z
2019-09-10T11:56:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1678-4405
http://dx.doi.org/10.1590/S1517-83822001000200013
https://locus.ufv.br//handle/123456789/26916
identifier_str_mv 1678-4405
url http://dx.doi.org/10.1590/S1517-83822001000200013
https://locus.ufv.br//handle/123456789/26916
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 32, n. 02, p. 135- 140, abr./ jun. 2001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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