Morphology and contractility in cardiomyocytes of rats with low exercise performance

Detalhes bibliográficos
Autor(a) principal: Quintão Júnior, Judson Fonseca
Data de Publicação: 2012
Outros Autores: Natali, Antônio José, Carneiro Júnior, Miguel Araújo, Castro, Cynthia Aparecida de, Drummond, Lucas Rios, Lavorato, Victor Neiva, Felix, Leonardo Bonato, Cruz, Jader dos Santos, Prímola-Gomes, Thales Nicolau
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0066-782X2012005000036
http://www.locus.ufv.br/handle/123456789/12489
Resumo: Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.
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spelling Quintão Júnior, Judson FonsecaNatali, Antônio JoséCarneiro Júnior, Miguel AraújoCastro, Cynthia Aparecida deDrummond, Lucas RiosLavorato, Victor NeivaFelix, Leonardo BonatoCruz, Jader dos SantosPrímola-Gomes, Thales Nicolau2017-10-30T11:00:21Z2017-10-30T11:00:21Z2012-01-240066-782Xhttp://dx.doi.org/10.1590/S0066-782X2012005000036http://www.locus.ufv.br/handle/123456789/12489Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.engArquivos Brasileiros de Cardiologiavol.98, n.5, p.431-436, Apr. 2012MyocytesCardiacRatsExerciseFatigueMyocardial contractionPhysical conditioning animalMorphology and contractility in cardiomyocytes of rats with low exercise performanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALen_aop03312.pdfen_aop03312.pdftexto completoapplication/pdf5050103https://locus.ufv.br//bitstream/123456789/12489/1/en_aop03312.pdfd983dd399f1c3406297548c101f97cffMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12489/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILen_aop03312.pdf.jpgen_aop03312.pdf.jpgIM Thumbnailimage/jpeg6381https://locus.ufv.br//bitstream/123456789/12489/3/en_aop03312.pdf.jpgfea5cb4fb572b0769311ba5aaebc0fb4MD53123456789/124892017-10-30 22:00:53.728oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-31T01:00:53LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Morphology and contractility in cardiomyocytes of rats with low exercise performance
title Morphology and contractility in cardiomyocytes of rats with low exercise performance
spellingShingle Morphology and contractility in cardiomyocytes of rats with low exercise performance
Quintão Júnior, Judson Fonseca
Myocytes
Cardiac
Rats
Exercise
Fatigue
Myocardial contraction
Physical conditioning animal
title_short Morphology and contractility in cardiomyocytes of rats with low exercise performance
title_full Morphology and contractility in cardiomyocytes of rats with low exercise performance
title_fullStr Morphology and contractility in cardiomyocytes of rats with low exercise performance
title_full_unstemmed Morphology and contractility in cardiomyocytes of rats with low exercise performance
title_sort Morphology and contractility in cardiomyocytes of rats with low exercise performance
author Quintão Júnior, Judson Fonseca
author_facet Quintão Júnior, Judson Fonseca
Natali, Antônio José
Carneiro Júnior, Miguel Araújo
Castro, Cynthia Aparecida de
Drummond, Lucas Rios
Lavorato, Victor Neiva
Felix, Leonardo Bonato
Cruz, Jader dos Santos
Prímola-Gomes, Thales Nicolau
author_role author
author2 Natali, Antônio José
Carneiro Júnior, Miguel Araújo
Castro, Cynthia Aparecida de
Drummond, Lucas Rios
Lavorato, Victor Neiva
Felix, Leonardo Bonato
Cruz, Jader dos Santos
Prímola-Gomes, Thales Nicolau
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Quintão Júnior, Judson Fonseca
Natali, Antônio José
Carneiro Júnior, Miguel Araújo
Castro, Cynthia Aparecida de
Drummond, Lucas Rios
Lavorato, Victor Neiva
Felix, Leonardo Bonato
Cruz, Jader dos Santos
Prímola-Gomes, Thales Nicolau
dc.subject.pt-BR.fl_str_mv Myocytes
Cardiac
Rats
Exercise
Fatigue
Myocardial contraction
Physical conditioning animal
topic Myocytes
Cardiac
Rats
Exercise
Fatigue
Myocardial contraction
Physical conditioning animal
description Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.
publishDate 2012
dc.date.issued.fl_str_mv 2012-01-24
dc.date.accessioned.fl_str_mv 2017-10-30T11:00:21Z
dc.date.available.fl_str_mv 2017-10-30T11:00:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0066-782X2012005000036
http://www.locus.ufv.br/handle/123456789/12489
dc.identifier.issn.none.fl_str_mv 0066-782X
identifier_str_mv 0066-782X
url http://dx.doi.org/10.1590/S0066-782X2012005000036
http://www.locus.ufv.br/handle/123456789/12489
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv vol.98, n.5, p.431-436, Apr. 2012
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