Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4619 |
Resumo: | Recombinant inbred lines (RIL s) are useful for building linkage maps. The maintainance the population genetic structure for mapping over their production is important for the construction of accurate maps. The existence of genetic variability within those populations is essential for detection of the association between molecular markers and controlling loci of quantitative or qualitative traits. Thus, the objective of this work was to quantify the diversity of a RIL population derived from Rudá x AND 277 cross. Five hundred plants from the F2 population of Rudá x AND 277 cross were conducted up to the F10 generation by the single seed descent method (SSD) to obtain those RIL s. Then, a group of 393 RIL's plus the parents and five control plants were evaluated in the field in a 20 x 20 triple lattice design, for seven quantitative traits. Because of the low efficiency of the lattice, the data were analyzed in randomized blocks with additional treatments (parents) with three replications. The five controls were eliminated from this analysis. The 393 RIL s and parents were characterized in the greenhouse for their reaction resistance to breeds 65 and 89 of Colletotrichum lindemunthianum, the causal agent of anthracnose. The effect of RIL s was significant for all traits evaluated (P < 0.01), indicating the existence of genetic variability in such population. The RIL s vs parents contrast was significant for number of days to flowering (DF) and to harvest (DC), grain yield (PROD) and weight of one hundred grains (M100) and not significant for architecture of plants (ARQ), degree of seed flattening (H) and seed shape (J). Significance of these contrasts indicates that the mean of RIL's differs from that of the parents. Coincidence between these means is expected only in the absence of epistasis. Thus, these results indicate the occurrence of additive x additive epistatic interactions for DF, DC, PROD and M100. The low values of the coefficient of experimental variation obtained indicate good precision of the experiment. Values of heritability ranged from 82.81 to 97.09%. In relation to breeds 65 and 89 of anthracnose, it was observed a 3:1 (resistant: susceptible) and 1:1 segregations respectively in the population of 393 RIL s, indicating the maintenance of genetic structure of such RIL s. The characters that formed the lowest and the highest number of groups of means were ARQ and M100, four and ten groups, respectively, by the Skott-Knott test at 5% probability. The 393 RIL s were grouped by Tocher method in 10 groups and the characters that most contributed to the genetic dissimilarity were M100 and DF, while ARQ was the character that contributed the least. The population made up by 393 RIL s present genetic variability for all traits, which is essential for detecting associations with molecular markers and genetic mapping. |
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Silva, Leonardo Corrêa dahttp://lattes.cnpq.br/6756716469546778Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Carneiro, Pedro Crescêncio Souzahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728227T6Carneiro, José Eustáquio de Souzahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783648T9Souza, Thiago Lívio Pessoa Oliveira dehttp://lattes.cnpq.br/96501833087791432015-03-26T13:40:00Z2014-04-042015-03-26T13:40:00Z2013-07-16SILVA, Leonardo Corrêa da. Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277. 2013. 60 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4619Recombinant inbred lines (RIL s) are useful for building linkage maps. The maintainance the population genetic structure for mapping over their production is important for the construction of accurate maps. The existence of genetic variability within those populations is essential for detection of the association between molecular markers and controlling loci of quantitative or qualitative traits. Thus, the objective of this work was to quantify the diversity of a RIL population derived from Rudá x AND 277 cross. Five hundred plants from the F2 population of Rudá x AND 277 cross were conducted up to the F10 generation by the single seed descent method (SSD) to obtain those RIL s. Then, a group of 393 RIL's plus the parents and five control plants were evaluated in the field in a 20 x 20 triple lattice design, for seven quantitative traits. Because of the low efficiency of the lattice, the data were analyzed in randomized blocks with additional treatments (parents) with three replications. The five controls were eliminated from this analysis. The 393 RIL s and parents were characterized in the greenhouse for their reaction resistance to breeds 65 and 89 of Colletotrichum lindemunthianum, the causal agent of anthracnose. The effect of RIL s was significant for all traits evaluated (P < 0.01), indicating the existence of genetic variability in such population. The RIL s vs parents contrast was significant for number of days to flowering (DF) and to harvest (DC), grain yield (PROD) and weight of one hundred grains (M100) and not significant for architecture of plants (ARQ), degree of seed flattening (H) and seed shape (J). Significance of these contrasts indicates that the mean of RIL's differs from that of the parents. Coincidence between these means is expected only in the absence of epistasis. Thus, these results indicate the occurrence of additive x additive epistatic interactions for DF, DC, PROD and M100. The low values of the coefficient of experimental variation obtained indicate good precision of the experiment. Values of heritability ranged from 82.81 to 97.09%. In relation to breeds 65 and 89 of anthracnose, it was observed a 3:1 (resistant: susceptible) and 1:1 segregations respectively in the population of 393 RIL s, indicating the maintenance of genetic structure of such RIL s. The characters that formed the lowest and the highest number of groups of means were ARQ and M100, four and ten groups, respectively, by the Skott-Knott test at 5% probability. The 393 RIL s were grouped by Tocher method in 10 groups and the characters that most contributed to the genetic dissimilarity were M100 and DF, while ARQ was the character that contributed the least. The population made up by 393 RIL s present genetic variability for all traits, which is essential for detecting associations with molecular markers and genetic mapping.Linhagens endogâmicas recombinantes (Recombinante Imbred Lines - RIL s) são de utilidade na construção de mapas de ligação. A manutenção da estrutura genética das populações para o mapeamento ao longo de sua obtenção é importante para a construção de mapas acurados. A existência de variabilidade genética nessas populações é fundamental para a detecção da associação entre marcadores moleculares e locos controladores de caracteres qualitativos ou quantitativos. Assim, o objetivo deste trabalho foi quantificar a diversidade de uma população de RIL s derivadas do cruzamento entre os genitores Rudá e AND 277. Na obtenção destas RIL s, 500 plantas F2 do cruzamento Rudá x AND 277 foram conduzidas até a geração F10 pelo método do descendente de uma única semente (Single Seed Descent - SSD). Em seguida, um grupo de 393 RIL s mais os genitores e cinco testemunhas foi avaliado em campo no delineamento látice triplo 20 x 20, quanto a sete caracteres quantitativos. Dada a baixa eficiência do látice, os dados foram analisados em blocos casualizados com testemunhas adicionais (genitores) e três repetições. As outras cinco testemunhas foram eliminadas dessa análise. As 393 RIL s e os genitores foram caracterizados quanto à reação de resistência às raças 65 e 89 de Colletotrichum lindemuthianum, causador da antracnose do feijoeiro, em casa-de- vegetação. Observou-se significância do efeito de RIL s para todos os caracteres avaliados (P < 0,01), indicando a existência de variabilidade genética nessa população. O contraste RIL s vs. Genitores foi significativo (P < 0,01) para os caracteres número de dias ao florescimento (DF) e à colheita (DC), produtividade de grãos (PROD) e massa de cem grãos (M100) e não significativo para a arquitetura de plantas (ARQ), grau de achatamento da semente (H) e forma da semente (J). A significância desses contrastes indica que a média das RIL s difere da média dos genitores. Igualdade entre as médias das RIL s e dos genitores é esperada apenas na ausência de epistasia. Assim, estes resultados indicam a ocorrência de interações epistáticas do tipo aditiva x aditiva para os caracteres DF, DC, PROD e M100. Os baixos valores do coeficiente de variação experimental obtidos indicam boa precisão do experimento. Os valores da herdabilidade variaram de 82,81 a 97,09%. Em relação às raças 65 e 89 de antracnose observou-se segregação de 3:1 (resistentes:suscetível) e de 1:1, respectivamente, na população de 393 RIL s, indicando manutenção da estrutura genética destas RIL s. Pelo teste de Skott-Knott, a 5% de probabilidade, os caracteres que formaram menor e maior número de grupos de médias foram ARQ e M100, com quatro e dez grupos cada, respectivamente. As 393 RIL s foram agrupadas pelo método de Tocher em 10 grupos e os caracteres que mais contribuíram para a dissimilaridade genética foram M100 e DF, e o que menos contribuiu foi ARQ. A população constituída de 393 RIL s apresenta variabilidade genética para todos os caracteres avaliados, imprescindível para detecção de associações com marcas moleculares e mapeamento genético.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em FitotecniaUFVBRPlantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita dePhaseolus vulgarisMapa de ligaçãoDiversidade genéticaPhaseolus vulgarisLinkage mapGenetic diversityCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIACaracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf927669https://locus.ufv.br//bitstream/123456789/4619/1/texto%20completo.pdff83f22161a7d44b4d00efbcaead43a73MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain106653https://locus.ufv.br//bitstream/123456789/4619/2/texto%20completo.pdf.txt4b7c7ca6c4a7c2a500239ff227bcb9fbMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3548https://locus.ufv.br//bitstream/123456789/4619/3/texto%20completo.pdf.jpga54971c945eca4db66440ac8de7ed814MD53123456789/46192016-04-10 23:11:42.69oai:locus.ufv.br:123456789/4619Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:11:42LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
title |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
spellingShingle |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 Silva, Leonardo Corrêa da Phaseolus vulgaris Mapa de ligação Diversidade genética Phaseolus vulgaris Linkage map Genetic diversity CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
title_short |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
title_full |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
title_fullStr |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
title_full_unstemmed |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
title_sort |
Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277 |
author |
Silva, Leonardo Corrêa da |
author_facet |
Silva, Leonardo Corrêa da |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/6756716469546778 |
dc.contributor.author.fl_str_mv |
Silva, Leonardo Corrêa da |
dc.contributor.advisor-co1.fl_str_mv |
Barros, Everaldo Gonçalves de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6 |
dc.contributor.advisor-co2.fl_str_mv |
Carneiro, Pedro Crescêncio Souza |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728227T6 |
dc.contributor.advisor1.fl_str_mv |
Carneiro, José Eustáquio de Souza |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783648T9 |
dc.contributor.referee1.fl_str_mv |
Souza, Thiago Lívio Pessoa Oliveira de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9650183308779143 |
contributor_str_mv |
Barros, Everaldo Gonçalves de Carneiro, Pedro Crescêncio Souza Carneiro, José Eustáquio de Souza Souza, Thiago Lívio Pessoa Oliveira de |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris Mapa de ligação Diversidade genética |
topic |
Phaseolus vulgaris Mapa de ligação Diversidade genética Phaseolus vulgaris Linkage map Genetic diversity CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
dc.subject.eng.fl_str_mv |
Phaseolus vulgaris Linkage map Genetic diversity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
description |
Recombinant inbred lines (RIL s) are useful for building linkage maps. The maintainance the population genetic structure for mapping over their production is important for the construction of accurate maps. The existence of genetic variability within those populations is essential for detection of the association between molecular markers and controlling loci of quantitative or qualitative traits. Thus, the objective of this work was to quantify the diversity of a RIL population derived from Rudá x AND 277 cross. Five hundred plants from the F2 population of Rudá x AND 277 cross were conducted up to the F10 generation by the single seed descent method (SSD) to obtain those RIL s. Then, a group of 393 RIL's plus the parents and five control plants were evaluated in the field in a 20 x 20 triple lattice design, for seven quantitative traits. Because of the low efficiency of the lattice, the data were analyzed in randomized blocks with additional treatments (parents) with three replications. The five controls were eliminated from this analysis. The 393 RIL s and parents were characterized in the greenhouse for their reaction resistance to breeds 65 and 89 of Colletotrichum lindemunthianum, the causal agent of anthracnose. The effect of RIL s was significant for all traits evaluated (P < 0.01), indicating the existence of genetic variability in such population. The RIL s vs parents contrast was significant for number of days to flowering (DF) and to harvest (DC), grain yield (PROD) and weight of one hundred grains (M100) and not significant for architecture of plants (ARQ), degree of seed flattening (H) and seed shape (J). Significance of these contrasts indicates that the mean of RIL's differs from that of the parents. Coincidence between these means is expected only in the absence of epistasis. Thus, these results indicate the occurrence of additive x additive epistatic interactions for DF, DC, PROD and M100. The low values of the coefficient of experimental variation obtained indicate good precision of the experiment. Values of heritability ranged from 82.81 to 97.09%. In relation to breeds 65 and 89 of anthracnose, it was observed a 3:1 (resistant: susceptible) and 1:1 segregations respectively in the population of 393 RIL s, indicating the maintenance of genetic structure of such RIL s. The characters that formed the lowest and the highest number of groups of means were ARQ and M100, four and ten groups, respectively, by the Skott-Knott test at 5% probability. The 393 RIL s were grouped by Tocher method in 10 groups and the characters that most contributed to the genetic dissimilarity were M100 and DF, while ARQ was the character that contributed the least. The population made up by 393 RIL s present genetic variability for all traits, which is essential for detecting associations with molecular markers and genetic mapping. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-07-16 |
dc.date.available.fl_str_mv |
2014-04-04 2015-03-26T13:40:00Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:40:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Leonardo Corrêa da. Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277. 2013. 60 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4619 |
identifier_str_mv |
SILVA, Leonardo Corrêa da. Caracterização fenotípica de RIL s de feijão derivadas da população Rudá x AND 277. 2013. 60 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013. |
url |
http://locus.ufv.br/handle/123456789/4619 |
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Universidade Federal de Viçosa |
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Mestrado em Fitotecnia |
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UFV |
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BR |
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Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de |
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Universidade Federal de Viçosa |
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