Efeito do zinco isolado ou associado à vitamina E sobre testiculos de ratos Wistar adultos

• Autor(a) principal: Silva, Ana Teresa César
• Data de Publicação: 2011
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/251
• topic: Testículo; Zinco; Vitamina E; Testis, Zinc, Vitamin E; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Antioxidants are molecules that can slow or prevent the oxidation of other substances. Effects have been described associations of different micronutrients that leverage in certain tissues or organs, leading to health benefits. Zinc and vitamin E (α-tocopherol) is considered an important antioxidant in cell membranes, and both have benefits in reproductive isolation, seen in studies of semen. The precise actions of zinc only or α-tocopherol as antioxidants on parameters of fertility are still unclear and they have not been evaluated when associated in the testis. The objective of this study was to evaluate the effect of zinc alone or associated with α-tocopherol (α-Toc) on the characteristics of components stereological testicular adult Wistar rats. We used 56 Wistar rats of reproductive age, divided into seven groups of eight animals. Six groups received Zn in drinking water. Three groups received 5, 10 and 20 mg Zn, while three other groups received the same dosage of Zn associated with 15 mg of α-Toc by gavage in three weekly doses. A control group of eight animals received only water. The diet was ad libitum during the 56 days of treatment. The first three groups and the control received 0.9% NaCl solution by gavage to standardize the procedures among the treatments. Morphometric analysis of the tubular and intertubular compartment was carried out using Image Pro Plus software. The averages were compared using the SNK test (Student Newman Keuls) with 5% tolerance. In seminiferous tubule, the treatment with 10 mg Zn increased in spermatocytes preleptotene / leptotene, round spermatids and the total number of germ cells. The association of Zn with 20 mg of α-Toc increased mitotic index. In the intertubular, mice treated with 5 mg of Zn showed an increase in the number of Leydig cells and macrophages and mice treated com10mg Zn associated with α-Toc had increased lymphatic space, the number of blood vessels and intertubular space. It is concluded that Zn acted alone, preferably in the cells, both in the tubule, and in the intertubular. The synergistic action of two antioxidants (Zn + Toc) was shown by the increase in intertubular space, lymphatic and blood vessels and also with increased mitotic index.