Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s11270-015-2630-0 http://www.locus.ufv.br/handle/123456789/24096 |
Resumo: | Lettuce plants were exposed to different toxic levels of arsenic (As) to induce an oxidative stress response, and the role of nitric oxide (NO) (provided as sodium nitroprusside (SNP)) as an attenuating agent of this stress condition was evaluated. Plants were treated with 50 μM of As with or without 100 μM SNP added to the nutrient solution. The hydrogen peroxide, superoxide anion, and malondialdehyde concentrations and enzymatic activities were measured. The increase in As concentration detected in the leaves was followed by a significant increase in H2O2 and malondialdehyde (MDA) concentrations. However, the presence of SPN promoted a reduction in the concentration of these oxidative agents and also reduced the translocation of As to the shoots. The enzymatic activities in the plants exposed to As were increased, which indicates the active participation of these enzymes in the reduction of oxidative stress induced by the metalloid. In the plants exposed to As and SNP, the enzymatic activities were not so high; this result was possibly related to the direct action of NO in scavenging the generated toxic metabolites and with the reduction in the translocation of the pollutant to the shoots. Lettuce and leaves of other vegetables are usually ingested, and this study shows an alternative to avoid human contamination with As. |
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Silveira, Neidiquele M.Canatto, Regiane A.Siman, LuhanOliveira, Juraci A. deRibeiro, ClebersonCambraia, JoséFarnese, Fernanda2019-03-25T16:32:44Z2019-03-25T16:32:44Z2015-111573-2932https://doi.org/10.1007/s11270-015-2630-0http://www.locus.ufv.br/handle/123456789/24096Lettuce plants were exposed to different toxic levels of arsenic (As) to induce an oxidative stress response, and the role of nitric oxide (NO) (provided as sodium nitroprusside (SNP)) as an attenuating agent of this stress condition was evaluated. Plants were treated with 50 μM of As with or without 100 μM SNP added to the nutrient solution. The hydrogen peroxide, superoxide anion, and malondialdehyde concentrations and enzymatic activities were measured. The increase in As concentration detected in the leaves was followed by a significant increase in H2O2 and malondialdehyde (MDA) concentrations. However, the presence of SPN promoted a reduction in the concentration of these oxidative agents and also reduced the translocation of As to the shoots. The enzymatic activities in the plants exposed to As were increased, which indicates the active participation of these enzymes in the reduction of oxidative stress induced by the metalloid. In the plants exposed to As and SNP, the enzymatic activities were not so high; this result was possibly related to the direct action of NO in scavenging the generated toxic metabolites and with the reduction in the translocation of the pollutant to the shoots. Lettuce and leaves of other vegetables are usually ingested, and this study shows an alternative to avoid human contamination with As.engWater, Air, & Soil PollutionVolumes 226, Issue 11, Articles 379, Pages 1-9, November 2015Springer International Publishing Switzerlandinfo:eu-repo/semantics/openAccessAntioxidative enzymesArsenic-contaminated foodPublic healthReactive oxygen speciesPlant physiologyNitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leavesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf648053https://locus.ufv.br//bitstream/123456789/24096/1/artigo.pdf6dd804d0ac631b8c176d5dc3e161d346MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24096/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/240962019-03-25 13:34:44.651oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-03-25T16:34:44LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
title |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
spellingShingle |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves Silveira, Neidiquele M. Antioxidative enzymes Arsenic-contaminated food Public health Reactive oxygen species Plant physiology |
title_short |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
title_full |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
title_fullStr |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
title_full_unstemmed |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
title_sort |
Nitric oxide attenuates oxidative stress induced by arsenic in lettuce (Lactuca sativa) leaves |
author |
Silveira, Neidiquele M. |
author_facet |
Silveira, Neidiquele M. Canatto, Regiane A. Siman, Luhan Oliveira, Juraci A. de Ribeiro, Cleberson Cambraia, José Farnese, Fernanda |
author_role |
author |
author2 |
Canatto, Regiane A. Siman, Luhan Oliveira, Juraci A. de Ribeiro, Cleberson Cambraia, José Farnese, Fernanda |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silveira, Neidiquele M. Canatto, Regiane A. Siman, Luhan Oliveira, Juraci A. de Ribeiro, Cleberson Cambraia, José Farnese, Fernanda |
dc.subject.pt-BR.fl_str_mv |
Antioxidative enzymes Arsenic-contaminated food Public health Reactive oxygen species Plant physiology |
topic |
Antioxidative enzymes Arsenic-contaminated food Public health Reactive oxygen species Plant physiology |
description |
Lettuce plants were exposed to different toxic levels of arsenic (As) to induce an oxidative stress response, and the role of nitric oxide (NO) (provided as sodium nitroprusside (SNP)) as an attenuating agent of this stress condition was evaluated. Plants were treated with 50 μM of As with or without 100 μM SNP added to the nutrient solution. The hydrogen peroxide, superoxide anion, and malondialdehyde concentrations and enzymatic activities were measured. The increase in As concentration detected in the leaves was followed by a significant increase in H2O2 and malondialdehyde (MDA) concentrations. However, the presence of SPN promoted a reduction in the concentration of these oxidative agents and also reduced the translocation of As to the shoots. The enzymatic activities in the plants exposed to As were increased, which indicates the active participation of these enzymes in the reduction of oxidative stress induced by the metalloid. In the plants exposed to As and SNP, the enzymatic activities were not so high; this result was possibly related to the direct action of NO in scavenging the generated toxic metabolites and with the reduction in the translocation of the pollutant to the shoots. Lettuce and leaves of other vegetables are usually ingested, and this study shows an alternative to avoid human contamination with As. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-11 |
dc.date.accessioned.fl_str_mv |
2019-03-25T16:32:44Z |
dc.date.available.fl_str_mv |
2019-03-25T16:32:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s11270-015-2630-0 http://www.locus.ufv.br/handle/123456789/24096 |
dc.identifier.issn.none.fl_str_mv |
1573-2932 |
identifier_str_mv |
1573-2932 |
url |
https://doi.org/10.1007/s11270-015-2630-0 http://www.locus.ufv.br/handle/123456789/24096 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volumes 226, Issue 11, Articles 379, Pages 1-9, November 2015 |
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Springer International Publishing Switzerland info:eu-repo/semantics/openAccess |
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Springer International Publishing Switzerland |
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openAccess |
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dc.publisher.none.fl_str_mv |
Water, Air, & Soil Pollution |
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Water, Air, & Soil Pollution |
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