Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA

Detalhes bibliográficos
Autor(a) principal: Prudêncio, Cláudia Vieira
Data de Publicação: 2015
Outros Autores: Ferreira, Sukarno Olavo, Mantovani, Hilário Cuquetto, Vanetti, Maria Cristina Dantas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1007/s13213-015-1117-y
http://www.locus.ufv.br/handle/123456789/21544
Resumo: Bacteriocins, particularly those produced by Gram-positive bacteria, have in recent years been considered promising antimicrobial agents to inhibit bacterial growth in food, and thus are potential food preservatives. These peptides generally exhibit a spectrum of action limited to Gram-positive bacteria. However, their action can be extended to Gram-negative bacteria through association with chelating agents. In the present study, we evaluated the occurrence of morphological changes on the cell envelope of Salmonella Typhimurium cells treated with bovicin HC5—a lantibiotic from Streptococcus bovis HC5—in association with EDTA. The morphological changes of the cells were visualized by atomic force microscopy (AFM), and the increase in cell membrane permeability was confirmed by the leakage of potassium ions (K+). The images displayed changes in the cell envelope, with increased surface roughness and a decreased cell volume. These changes indicate that EDTA plays a role in the destabilization of the outer membrane, allowing bovicin HC5 to act on the cytoplasmic membrane through the formation of pores, which was confirmed by the detection of potassium in the cell supernatant. These results suggest that bovicin HC5 combined with EDTA has potential for use on Salmonella cells.
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spelling Prudêncio, Cláudia VieiraFerreira, Sukarno OlavoMantovani, Hilário CuquettoVanetti, Maria Cristina Dantas2018-08-30T16:57:25Z2018-08-30T16:57:25Z2015-07-0318692044http://dx.doi.org/10.1007/s13213-015-1117-yhttp://www.locus.ufv.br/handle/123456789/21544Bacteriocins, particularly those produced by Gram-positive bacteria, have in recent years been considered promising antimicrobial agents to inhibit bacterial growth in food, and thus are potential food preservatives. These peptides generally exhibit a spectrum of action limited to Gram-positive bacteria. However, their action can be extended to Gram-negative bacteria through association with chelating agents. In the present study, we evaluated the occurrence of morphological changes on the cell envelope of Salmonella Typhimurium cells treated with bovicin HC5—a lantibiotic from Streptococcus bovis HC5—in association with EDTA. The morphological changes of the cells were visualized by atomic force microscopy (AFM), and the increase in cell membrane permeability was confirmed by the leakage of potassium ions (K+). The images displayed changes in the cell envelope, with increased surface roughness and a decreased cell volume. These changes indicate that EDTA plays a role in the destabilization of the outer membrane, allowing bovicin HC5 to act on the cytoplasmic membrane through the formation of pores, which was confirmed by the detection of potassium in the cell supernatant. These results suggest that bovicin HC5 combined with EDTA has potential for use on Salmonella cells.engAnnals of Microbiologyv. 66, n. 1, p. 373– 379, mar. 2016Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessBacteriocinsBiocontrolLantibioticsAtomic force microscopyOuter membraneGram-negative bacteriaMorphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf7034354https://locus.ufv.br//bitstream/123456789/21544/1/artigo.pdf6c89bb603928454fb9751191d9cb668dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21544/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5072https://locus.ufv.br//bitstream/123456789/21544/3/artigo.pdf.jpg3d655df36fb187bd9214d64355510d8aMD53123456789/215442018-08-30 23:00:48.909oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-08-31T02:00:48LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
title Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
spellingShingle Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
Prudêncio, Cláudia Vieira
Bacteriocins
Biocontrol
Lantibiotics
Atomic force microscopy
Outer membrane
Gram-negative bacteria
title_short Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
title_full Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
title_fullStr Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
title_full_unstemmed Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
title_sort Morphological changes in Salmonella Typhimurium caused by the lantibiotic bovicin HC5 in association with EDTA
author Prudêncio, Cláudia Vieira
author_facet Prudêncio, Cláudia Vieira
Ferreira, Sukarno Olavo
Mantovani, Hilário Cuquetto
Vanetti, Maria Cristina Dantas
author_role author
author2 Ferreira, Sukarno Olavo
Mantovani, Hilário Cuquetto
Vanetti, Maria Cristina Dantas
author2_role author
author
author
dc.contributor.author.fl_str_mv Prudêncio, Cláudia Vieira
Ferreira, Sukarno Olavo
Mantovani, Hilário Cuquetto
Vanetti, Maria Cristina Dantas
dc.subject.pt-BR.fl_str_mv Bacteriocins
Biocontrol
Lantibiotics
Atomic force microscopy
Outer membrane
Gram-negative bacteria
topic Bacteriocins
Biocontrol
Lantibiotics
Atomic force microscopy
Outer membrane
Gram-negative bacteria
description Bacteriocins, particularly those produced by Gram-positive bacteria, have in recent years been considered promising antimicrobial agents to inhibit bacterial growth in food, and thus are potential food preservatives. These peptides generally exhibit a spectrum of action limited to Gram-positive bacteria. However, their action can be extended to Gram-negative bacteria through association with chelating agents. In the present study, we evaluated the occurrence of morphological changes on the cell envelope of Salmonella Typhimurium cells treated with bovicin HC5—a lantibiotic from Streptococcus bovis HC5—in association with EDTA. The morphological changes of the cells were visualized by atomic force microscopy (AFM), and the increase in cell membrane permeability was confirmed by the leakage of potassium ions (K+). The images displayed changes in the cell envelope, with increased surface roughness and a decreased cell volume. These changes indicate that EDTA plays a role in the destabilization of the outer membrane, allowing bovicin HC5 to act on the cytoplasmic membrane through the formation of pores, which was confirmed by the detection of potassium in the cell supernatant. These results suggest that bovicin HC5 combined with EDTA has potential for use on Salmonella cells.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-03
dc.date.accessioned.fl_str_mv 2018-08-30T16:57:25Z
dc.date.available.fl_str_mv 2018-08-30T16:57:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s13213-015-1117-y
http://www.locus.ufv.br/handle/123456789/21544
dc.identifier.issn.none.fl_str_mv 18692044
identifier_str_mv 18692044
url http://dx.doi.org/10.1007/s13213-015-1117-y
http://www.locus.ufv.br/handle/123456789/21544
dc.language.iso.fl_str_mv eng
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dc.relation.ispartofseries.pt-BR.fl_str_mv v. 66, n. 1, p. 373– 379, mar. 2016
dc.rights.driver.fl_str_mv Springer Nature Switzerland AG.
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dc.publisher.none.fl_str_mv Annals of Microbiology
publisher.none.fl_str_mv Annals of Microbiology
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