Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes

Detalhes bibliográficos
Autor(a) principal: Careli, Roberta Torres
Data de Publicação: 2009
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/420
Resumo: The occurrence of Cryptosporidium spp. and Giardia spp was evaluated on plantations of oily lettuce, variety Vitoria de Santo Antão, on the countryside of Viçosa, Minas Gerais state, on dry and wet seasons. It was found both protozoans in the water, on lettuce, organic fertilizer and soil, at least in one plantation and one season. The main contamination source of both protozoans on lettuce was the water used on the irrigation. The number of microorganisms was affected by rainfall variations, and higher values were observed on the wet season. The microbiological contamination of water, lettuce, organic manure and soil was shown by different bacteriological analysis. It was observed high correlation between the occurrence of Cryptosporidium spp. and Giardia spp. in all the types of samples, except by the fertilizer. High correlations between the protozoans and C. perfringens were found in water samples. The results denoted that some bacteriological indicators, such as the coliform group, cannot predict the presence of protozoans. Besides that, the turbidity of the different sources of water presented higher values on the wet season, which shows high correlation with the presence of Cryptosporidium spp. e Giardia spp. in irrigation water. The ability to remove Cryptosporidium parvum adhered to lettuce leaves, as pure culture and mixed with Pseudomonas fluorescens, was evaluated by using solutions of different concentrations of benzalkonium chloride, Tween 80, and sodium dodecyl sulphate (SDS), after 10 minutes of contact. It was verified that SDS presented better protozoans removal, and its effectiveness increased as the concentration (p < 0,05). It was evaluated the ability to capture and retain oocysts of C. parvum by P. fluorescens biofilms established on polyvinylchloride (PVC) pipes. The number of captured oocysts was higher (p < 0,05) than the number of retained oocysts after increasing the flow in six times. It was verified that the oocysts present little ability to adhere to the PVC surface. The thermodynamic features related to the formation of P. fluorescens biofilm and C. parvum oocysts adhesion on PVC pipes were also evaluated. It was observed that the surfaces of C. parvum and P. fluorescens are hydrophilic, whereas PVC is a hydrophobic material. Such results were confirmed by quantifying the surface free energy (&#916;Gsws).The determination of the total free interaction energy denoted that the adhesion of P. fluorescens biofilm and C. parvum oocysts was thermodynamically favorable (&#916;GadesãoTOT < 0) for all the conditions. The control of P. fluorescens biofilm and C. parvum oocysts adhered to PVC pipes was carried out by applying benzalkonium chloride.The tested concentrations of benzalkonium chloride leaded to an increase (P < 0,05) on the DR number of both microorganisms, as they were submitted to higher concentrations of surfactants. The application of benzalkonium chloride on higienization procedures can be an alternative to control bacteria and protozoan adhered to PVC pipes used on vegetable irrigation. However, some toxicological tests are necessary in order to evaluate the prejudicial effect of the suggested concentration.
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spelling Careli, Roberta Torreshttp://lattes.cnpq.br/5153043513212696Minim, Luis Antoniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4789633Y8Ribeiro Junior, José Ivohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723282Y6Andrade, Nélio José dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788281Y5Pinto, Cláudia Lúcia de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783521J6Martins, Maurilio Lopeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794520A8Silva, Washington Azevedo dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765007Y52015-03-26T12:24:57Z2011-06-022015-03-26T12:24:57Z2009-10-29CARELI, Roberta Torres. Occurrence of Cryptosporidium spp. in the chain production of lettuce (Lactuca sativa L.), its capture and retention by Pseudomonas fluorescens biolfim and its control by surfactants. 2009. 177 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/420The occurrence of Cryptosporidium spp. and Giardia spp was evaluated on plantations of oily lettuce, variety Vitoria de Santo Antão, on the countryside of Viçosa, Minas Gerais state, on dry and wet seasons. It was found both protozoans in the water, on lettuce, organic fertilizer and soil, at least in one plantation and one season. The main contamination source of both protozoans on lettuce was the water used on the irrigation. The number of microorganisms was affected by rainfall variations, and higher values were observed on the wet season. The microbiological contamination of water, lettuce, organic manure and soil was shown by different bacteriological analysis. It was observed high correlation between the occurrence of Cryptosporidium spp. and Giardia spp. in all the types of samples, except by the fertilizer. High correlations between the protozoans and C. perfringens were found in water samples. The results denoted that some bacteriological indicators, such as the coliform group, cannot predict the presence of protozoans. Besides that, the turbidity of the different sources of water presented higher values on the wet season, which shows high correlation with the presence of Cryptosporidium spp. e Giardia spp. in irrigation water. The ability to remove Cryptosporidium parvum adhered to lettuce leaves, as pure culture and mixed with Pseudomonas fluorescens, was evaluated by using solutions of different concentrations of benzalkonium chloride, Tween 80, and sodium dodecyl sulphate (SDS), after 10 minutes of contact. It was verified that SDS presented better protozoans removal, and its effectiveness increased as the concentration (p < 0,05). It was evaluated the ability to capture and retain oocysts of C. parvum by P. fluorescens biofilms established on polyvinylchloride (PVC) pipes. The number of captured oocysts was higher (p < 0,05) than the number of retained oocysts after increasing the flow in six times. It was verified that the oocysts present little ability to adhere to the PVC surface. The thermodynamic features related to the formation of P. fluorescens biofilm and C. parvum oocysts adhesion on PVC pipes were also evaluated. It was observed that the surfaces of C. parvum and P. fluorescens are hydrophilic, whereas PVC is a hydrophobic material. Such results were confirmed by quantifying the surface free energy (&#916;Gsws).The determination of the total free interaction energy denoted that the adhesion of P. fluorescens biofilm and C. parvum oocysts was thermodynamically favorable (&#916;GadesãoTOT < 0) for all the conditions. The control of P. fluorescens biofilm and C. parvum oocysts adhered to PVC pipes was carried out by applying benzalkonium chloride.The tested concentrations of benzalkonium chloride leaded to an increase (P < 0,05) on the DR number of both microorganisms, as they were submitted to higher concentrations of surfactants. The application of benzalkonium chloride on higienization procedures can be an alternative to control bacteria and protozoan adhered to PVC pipes used on vegetable irrigation. However, some toxicological tests are necessary in order to evaluate the prejudicial effect of the suggested concentration.A ocorrência de Cryptosporidium spp. e Giardia spp. em unidades produtoras de alface lisa, cultivar Vitória de Santo Antão, situadas na zona rural de Viçosa, Minas Gerais, foi avaliada nos períodos de seca e de águas. Constatou-se a presença de ambos os protozoários em água, alface, adubo orgânico e solo em, pelo menos, uma unidade produtora de alface e um período. A principal fonte de contaminação de ambos protozoários em alface foi a água usada para irrigação e os números desses microrganismos tiveram influência das variações pluviométricas (p < 0,05), sendo mais elevados no período de águas. A contaminação microbiológica de água, alface, adubo orgânico e solo também foi mostrada por diferentes análises bacteriológicas. Foram observadas correlações elevadas entre a ocorrência de Cryptosporidium spp. e Giardia spp. em todos os tipos de amostras, com exceção do adubo. Em água também foram constatadas boas correlações entre protozoários e C. perfringens. Os resultados indicaram que alguns indicadores bacteriológicos, como o grupo coliforme, não são preditivos para a presença de protozoários. Além disso, os valores de turbidez das diferentes fontes de água pertencentes às unidades produtoras apresentaram-se mais elevados durante a época de águas e apresentaram correlação alta com a presença de Cryptosporidium spp. e Giardia spp. em água de irrigação. A capacidade de remoção de Cryptosporidium parvum em cultura pura ou mista com Pseudomonas fluorescens aderidos por 12 h em cupons de folhas de alface foi avaliada pela utilização de soluções com diferentes concentrações de cloreto de benzalcônio, Tween 80 e dodecil sulfato de sódio (SDS) por 10 min de contato. Constatou-se que SDS apresentou uma maior remoção dos protozoários e sua efetividade aumentou com a concentração (p < 0,05). Avaliou-se a capacidade de oocistos de C. parvum serem capturados e retidos por biofilmes de P. fluorescens formados em tubulação de poli (cloreto de vinila), PVC. O número de oocistos capturados pelo biofilme foi maior (p < 0,05) do que o de oocistos retidos após o aumento de seis vezes do fluxo do líquido circulante. Constatou-se que, na ausência do biofilme bacteriano, os oocistos possuem pouca capacidade de adesão à superfície de PVC. Os aspectos termodinâmicos na formação de biofilmes de P. fluorescens e de oocistos de C. parvum aderidos à tubulação de PVC também foram avaliados. Observou-se que as superfícies de C. parvum e P. fluorescens apresentaram caráter hidrofílico, ao contrário do PVC que é hidrofóbico. Esses resultados foram confirmados pela quantificação da energia livre de superfície (&#916;Gsws). A quantificação da energia livre total de interação indicou que a adesão de P. fluorescens na forma de biofilmes e de oocistos de C. parvum foi termodinamicamente favorável (&#916;GadesãoTOT < 0) em todas as condições. O controle de biofilmes de P. fluorescens e de oocistos de C. parvum aderidos em tubulação de PVC foi realizado por meio de aplicação de cloreto de benzalcônio. As concentrações testadas das soluções de cloreto de benzalcônio resultaram em aumento (P < 0,05) no número de RD de ambos os microrganismos à medida que os mesmos foram submetidos a tratamentos com soluções surfactantes em concentrações mais elevadas. A aplicação de soluções de cloreto de benzalcônio em procedimentos de higienização pode ser uma alternativa de controle de bactérias e protozoários aderidos à tubulação de PVC utilizada na irrigação de hortaliças. Contudo, são necessários testes toxicológicos para avaliar o efeito nocivo desse produto na concentração sugerida.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em Ciência e Tecnologia de AlimentosUFVBRCiência de Alimentos; Tecnologia de Alimentos; Engenharia de AlimentosCryptosporidium e GiardiaAlfaceHigienizaçãoCryptosporidium and GiardiaLettuceHigienizationCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSOcorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantesOccurrence of Cryptosporidium spp. in the chain production of lettuce (Lactuca sativa L.), its capture and retention by Pseudomonas fluorescens biolfim and its control by surfactantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1348385https://locus.ufv.br//bitstream/123456789/420/1/texto%20completo.pdfc331d2e697898fb6f4bfa39e2252ef42MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain353368https://locus.ufv.br//bitstream/123456789/420/2/texto%20completo.pdf.txt9dea098dbd79a7f57ce0cb55980958aaMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3747https://locus.ufv.br//bitstream/123456789/420/3/texto%20completo.pdf.jpg5f0fe17156a8527fa1c56d04edda20dfMD53123456789/4202016-04-06 23:04:52.981oai:locus.ufv.br:123456789/420Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:04:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
dc.title.alternative.eng.fl_str_mv Occurrence of Cryptosporidium spp. in the chain production of lettuce (Lactuca sativa L.), its capture and retention by Pseudomonas fluorescens biolfim and its control by surfactants
title Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
spellingShingle Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
Careli, Roberta Torres
Cryptosporidium e Giardia
Alface
Higienização
Cryptosporidium and Giardia
Lettuce
Higienization
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
title_short Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
title_full Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
title_fullStr Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
title_full_unstemmed Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
title_sort Ocorrência de Cryptosporidium spp. na cadeia produtiva de alface (Lactuca sativa L.), sua captura e retenção por biofilmes de Pseudomonas fluorescens e seu controle por surfactantes
author Careli, Roberta Torres
author_facet Careli, Roberta Torres
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5153043513212696
dc.contributor.author.fl_str_mv Careli, Roberta Torres
dc.contributor.advisor-co1.fl_str_mv Minim, Luis Antonio
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4789633Y8
dc.contributor.advisor-co2.fl_str_mv Ribeiro Junior, José Ivo
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723282Y6
dc.contributor.advisor1.fl_str_mv Andrade, Nélio José de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788281Y5
dc.contributor.referee1.fl_str_mv Pinto, Cláudia Lúcia de Oliveira
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783521J6
dc.contributor.referee2.fl_str_mv Martins, Maurilio Lopes
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794520A8
dc.contributor.referee3.fl_str_mv Silva, Washington Azevedo da
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765007Y5
contributor_str_mv Minim, Luis Antonio
Ribeiro Junior, José Ivo
Andrade, Nélio José de
Pinto, Cláudia Lúcia de Oliveira
Martins, Maurilio Lopes
Silva, Washington Azevedo da
dc.subject.por.fl_str_mv Cryptosporidium e Giardia
Alface
Higienização
topic Cryptosporidium e Giardia
Alface
Higienização
Cryptosporidium and Giardia
Lettuce
Higienization
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Cryptosporidium and Giardia
Lettuce
Higienization
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
description The occurrence of Cryptosporidium spp. and Giardia spp was evaluated on plantations of oily lettuce, variety Vitoria de Santo Antão, on the countryside of Viçosa, Minas Gerais state, on dry and wet seasons. It was found both protozoans in the water, on lettuce, organic fertilizer and soil, at least in one plantation and one season. The main contamination source of both protozoans on lettuce was the water used on the irrigation. The number of microorganisms was affected by rainfall variations, and higher values were observed on the wet season. The microbiological contamination of water, lettuce, organic manure and soil was shown by different bacteriological analysis. It was observed high correlation between the occurrence of Cryptosporidium spp. and Giardia spp. in all the types of samples, except by the fertilizer. High correlations between the protozoans and C. perfringens were found in water samples. The results denoted that some bacteriological indicators, such as the coliform group, cannot predict the presence of protozoans. Besides that, the turbidity of the different sources of water presented higher values on the wet season, which shows high correlation with the presence of Cryptosporidium spp. e Giardia spp. in irrigation water. The ability to remove Cryptosporidium parvum adhered to lettuce leaves, as pure culture and mixed with Pseudomonas fluorescens, was evaluated by using solutions of different concentrations of benzalkonium chloride, Tween 80, and sodium dodecyl sulphate (SDS), after 10 minutes of contact. It was verified that SDS presented better protozoans removal, and its effectiveness increased as the concentration (p < 0,05). It was evaluated the ability to capture and retain oocysts of C. parvum by P. fluorescens biofilms established on polyvinylchloride (PVC) pipes. The number of captured oocysts was higher (p < 0,05) than the number of retained oocysts after increasing the flow in six times. It was verified that the oocysts present little ability to adhere to the PVC surface. The thermodynamic features related to the formation of P. fluorescens biofilm and C. parvum oocysts adhesion on PVC pipes were also evaluated. It was observed that the surfaces of C. parvum and P. fluorescens are hydrophilic, whereas PVC is a hydrophobic material. Such results were confirmed by quantifying the surface free energy (&#916;Gsws).The determination of the total free interaction energy denoted that the adhesion of P. fluorescens biofilm and C. parvum oocysts was thermodynamically favorable (&#916;GadesãoTOT < 0) for all the conditions. The control of P. fluorescens biofilm and C. parvum oocysts adhered to PVC pipes was carried out by applying benzalkonium chloride.The tested concentrations of benzalkonium chloride leaded to an increase (P < 0,05) on the DR number of both microorganisms, as they were submitted to higher concentrations of surfactants. The application of benzalkonium chloride on higienization procedures can be an alternative to control bacteria and protozoan adhered to PVC pipes used on vegetable irrigation. However, some toxicological tests are necessary in order to evaluate the prejudicial effect of the suggested concentration.
publishDate 2009
dc.date.issued.fl_str_mv 2009-10-29
dc.date.available.fl_str_mv 2011-06-02
2015-03-26T12:24:57Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:24:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARELI, Roberta Torres. Occurrence of Cryptosporidium spp. in the chain production of lettuce (Lactuca sativa L.), its capture and retention by Pseudomonas fluorescens biolfim and its control by surfactants. 2009. 177 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2009.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/420
identifier_str_mv CARELI, Roberta Torres. Occurrence of Cryptosporidium spp. in the chain production of lettuce (Lactuca sativa L.), its capture and retention by Pseudomonas fluorescens biolfim and its control by surfactants. 2009. 177 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2009.
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