Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s00253-016-7902-0 http://www.locus.ufv.br/handle/123456789/19131 |
Resumo: | Water generated during oil exploration is chemically complex and contains high concentrations of ammonium and, in some cases, high salinity. The most common way to remove ammonium from effluent is a biological process, which can be performed by different routes and different groups of microorganisms. However, the presence of salts in the effluents could be an inhibiting factor for biological processes, interfering directly with treatment. This study aimed to evaluate changes in the profile of a microbial community involved in the process of ammonium removal when subjected to a gradual increase of salt (NaCl), in which the complete inhibition of the ammonium removal process occurred at 125 g L−1 NaCl. During the sludge acclimatization process, samples were collected and submitted to denaturing gradient gel electrophoresis (DGGE) and massive sequencing of the 16S ribosomal RNA (rRNA) genes. As the salt concentration increased in the reactor, a change in the microbial community was observed by the DGGE band profiles. As a result, there was a reduction in the presence of bacterial populations, and an increase in archaeal populations was found. The sequencing data suggested that ammonium removal in the reactor was carried out by different metabolic routes by autotrophic nitrifying bacteria, such as Nitrosococcus, Nitrosomonas, Nitrosovibrio, Nitrospira, and Nitrococcus; ammonium-oxidizing archaea Candidatus nitrosoarchaeum; ANAMMOX microorganisms, such as Candidatus brocadia, Candidatus kuenenia, and Candidatus scalindua; and microorganisms with the potential to be heterotrophic nitrifying, such as Paracoccus spp., Pseudomonas spp., Bacillus spp., Marinobacter sp., and Alcaligenes spp. |
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Quartaroli, LarissaSilva, Lívia C. FidélisSilva, Claudio MudaduLima, Helena SantiagoPaula, Sergio Oliveira deOliveira, Valéria Maia deSilva, Marliane de Cássia S. daKasuya, Maria Catarina M.Sousa, Maíra Paula deTorres, Ana Paula R.Souza, Rodrigo SuhettBassin, João PauloSilva, Cynthia Canêdo da2018-04-25T15:41:53Z2018-04-25T15:41:53Z2016-11-0414320614http://dx.doi.org/10.1007/s00253-016-7902-0http://www.locus.ufv.br/handle/123456789/19131Water generated during oil exploration is chemically complex and contains high concentrations of ammonium and, in some cases, high salinity. The most common way to remove ammonium from effluent is a biological process, which can be performed by different routes and different groups of microorganisms. However, the presence of salts in the effluents could be an inhibiting factor for biological processes, interfering directly with treatment. This study aimed to evaluate changes in the profile of a microbial community involved in the process of ammonium removal when subjected to a gradual increase of salt (NaCl), in which the complete inhibition of the ammonium removal process occurred at 125 g L−1 NaCl. During the sludge acclimatization process, samples were collected and submitted to denaturing gradient gel electrophoresis (DGGE) and massive sequencing of the 16S ribosomal RNA (rRNA) genes. As the salt concentration increased in the reactor, a change in the microbial community was observed by the DGGE band profiles. As a result, there was a reduction in the presence of bacterial populations, and an increase in archaeal populations was found. The sequencing data suggested that ammonium removal in the reactor was carried out by different metabolic routes by autotrophic nitrifying bacteria, such as Nitrosococcus, Nitrosomonas, Nitrosovibrio, Nitrospira, and Nitrococcus; ammonium-oxidizing archaea Candidatus nitrosoarchaeum; ANAMMOX microorganisms, such as Candidatus brocadia, Candidatus kuenenia, and Candidatus scalindua; and microorganisms with the potential to be heterotrophic nitrifying, such as Paracoccus spp., Pseudomonas spp., Bacillus spp., Marinobacter sp., and Alcaligenes spp.engAppl Microbiol Biotechnolv. 101, n. 2, p. 859–870, Janeiro 2017Springer-Verlag Berlin Heidelberginfo:eu-repo/semantics/openAccessMicrobial diversityNitrificationHeterotrophic nitrification16S rRNA gene surveyAmmonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1146404https://locus.ufv.br//bitstream/123456789/19131/1/artigo.pdf57841ef80c2744a705df3d08f719dc3fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19131/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4766https://locus.ufv.br//bitstream/123456789/19131/3/artigo.pdf.jpgf5c36c34f0a2b3b729f08e35912498ecMD53123456789/191312018-04-25 23:00:51.239oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-26T02:00:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
title |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
spellingShingle |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations Quartaroli, Larissa Microbial diversity Nitrification Heterotrophic nitrification 16S rRNA gene survey |
title_short |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
title_full |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
title_fullStr |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
title_full_unstemmed |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
title_sort |
Ammonium removal from high-salinity oilfield-produced water: assessing the microbial community dynamics at increasing salt concentrations |
author |
Quartaroli, Larissa |
author_facet |
Quartaroli, Larissa Silva, Lívia C. Fidélis Silva, Claudio Mudadu Lima, Helena Santiago Paula, Sergio Oliveira de Oliveira, Valéria Maia de Silva, Marliane de Cássia S. da Kasuya, Maria Catarina M. Sousa, Maíra Paula de Torres, Ana Paula R. Souza, Rodrigo Suhett Bassin, João Paulo Silva, Cynthia Canêdo da |
author_role |
author |
author2 |
Silva, Lívia C. Fidélis Silva, Claudio Mudadu Lima, Helena Santiago Paula, Sergio Oliveira de Oliveira, Valéria Maia de Silva, Marliane de Cássia S. da Kasuya, Maria Catarina M. Sousa, Maíra Paula de Torres, Ana Paula R. Souza, Rodrigo Suhett Bassin, João Paulo Silva, Cynthia Canêdo da |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Quartaroli, Larissa Silva, Lívia C. Fidélis Silva, Claudio Mudadu Lima, Helena Santiago Paula, Sergio Oliveira de Oliveira, Valéria Maia de Silva, Marliane de Cássia S. da Kasuya, Maria Catarina M. Sousa, Maíra Paula de Torres, Ana Paula R. Souza, Rodrigo Suhett Bassin, João Paulo Silva, Cynthia Canêdo da |
dc.subject.pt-BR.fl_str_mv |
Microbial diversity Nitrification Heterotrophic nitrification 16S rRNA gene survey |
topic |
Microbial diversity Nitrification Heterotrophic nitrification 16S rRNA gene survey |
description |
Water generated during oil exploration is chemically complex and contains high concentrations of ammonium and, in some cases, high salinity. The most common way to remove ammonium from effluent is a biological process, which can be performed by different routes and different groups of microorganisms. However, the presence of salts in the effluents could be an inhibiting factor for biological processes, interfering directly with treatment. This study aimed to evaluate changes in the profile of a microbial community involved in the process of ammonium removal when subjected to a gradual increase of salt (NaCl), in which the complete inhibition of the ammonium removal process occurred at 125 g L−1 NaCl. During the sludge acclimatization process, samples were collected and submitted to denaturing gradient gel electrophoresis (DGGE) and massive sequencing of the 16S ribosomal RNA (rRNA) genes. As the salt concentration increased in the reactor, a change in the microbial community was observed by the DGGE band profiles. As a result, there was a reduction in the presence of bacterial populations, and an increase in archaeal populations was found. The sequencing data suggested that ammonium removal in the reactor was carried out by different metabolic routes by autotrophic nitrifying bacteria, such as Nitrosococcus, Nitrosomonas, Nitrosovibrio, Nitrospira, and Nitrococcus; ammonium-oxidizing archaea Candidatus nitrosoarchaeum; ANAMMOX microorganisms, such as Candidatus brocadia, Candidatus kuenenia, and Candidatus scalindua; and microorganisms with the potential to be heterotrophic nitrifying, such as Paracoccus spp., Pseudomonas spp., Bacillus spp., Marinobacter sp., and Alcaligenes spp. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-11-04 |
dc.date.accessioned.fl_str_mv |
2018-04-25T15:41:53Z |
dc.date.available.fl_str_mv |
2018-04-25T15:41:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00253-016-7902-0 http://www.locus.ufv.br/handle/123456789/19131 |
dc.identifier.issn.none.fl_str_mv |
14320614 |
identifier_str_mv |
14320614 |
url |
http://dx.doi.org/10.1007/s00253-016-7902-0 http://www.locus.ufv.br/handle/123456789/19131 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 101, n. 2, p. 859–870, Janeiro 2017 |
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Springer-Verlag Berlin Heidelberg info:eu-repo/semantics/openAccess |
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Springer-Verlag Berlin Heidelberg |
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openAccess |
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Appl Microbiol Biotechnol |
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Appl Microbiol Biotechnol |
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LOCUS Repositório Institucional da UFV |
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