Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.3732/apps.1300003 http://www.locus.ufv.br/handle/123456789/12673 |
Resumo: | Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii . The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species. Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii . The microsatellite markers presented here will be useful in studies of contemporary and historical gene fl ow between American and West African Rhizophora species. |
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RIBEIRO, DIANA O.VINSON, CHRISTINA C.NASCIMENTO, DULCIVANIA S. S.MEHLIG, ULFMENEZES, MOIRAH P. M.SAMPAIO, IRACILDASILVA, MARIVANA B.2017-11-01T10:40:18Z2017-11-01T10:40:18Z2013-09-0221680450https://doi.org/10.3732/apps.1300003http://www.locus.ufv.br/handle/123456789/12673Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii . The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species. Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii . The microsatellite markers presented here will be useful in studies of contemporary and historical gene fl ow between American and West African Rhizophora species.engBioOne1(9): 1300003, September 2013MangroveMicrosatellitesRhizophora harrisoniiRhizophora racemosaRhizophoraceaeIsolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALapps.1300003.pdfapps.1300003.pdfTexto completoapplication/pdf71460https://locus.ufv.br//bitstream/123456789/12673/1/apps.1300003.pdfc4ba6648446d14d37a7072f5f15adf9eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12673/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILapps.1300003.pdf.jpgapps.1300003.pdf.jpgIM Thumbnailimage/jpeg4499https://locus.ufv.br//bitstream/123456789/12673/3/apps.1300003.pdf.jpg033635c45a0cad5d52d5a0ded31a19cbMD53123456789/126732017-11-01 22:00:45.132oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-02T01:00:45LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
title |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
spellingShingle |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) RIBEIRO, DIANA O. Mangrove Microsatellites Rhizophora harrisonii Rhizophora racemosa Rhizophoraceae |
title_short |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
title_full |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
title_fullStr |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
title_full_unstemmed |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
title_sort |
Isolation of Microsatellite Markers for the Red Mangrove, Rhizophora mangle (Rhizophoraceae) |
author |
RIBEIRO, DIANA O. |
author_facet |
RIBEIRO, DIANA O. VINSON, CHRISTINA C. NASCIMENTO, DULCIVANIA S. S. MEHLIG, ULF MENEZES, MOIRAH P. M. SAMPAIO, IRACILDA SILVA, MARIVANA B. |
author_role |
author |
author2 |
VINSON, CHRISTINA C. NASCIMENTO, DULCIVANIA S. S. MEHLIG, ULF MENEZES, MOIRAH P. M. SAMPAIO, IRACILDA SILVA, MARIVANA B. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
RIBEIRO, DIANA O. VINSON, CHRISTINA C. NASCIMENTO, DULCIVANIA S. S. MEHLIG, ULF MENEZES, MOIRAH P. M. SAMPAIO, IRACILDA SILVA, MARIVANA B. |
dc.subject.pt-BR.fl_str_mv |
Mangrove Microsatellites Rhizophora harrisonii Rhizophora racemosa Rhizophoraceae |
topic |
Mangrove Microsatellites Rhizophora harrisonii Rhizophora racemosa Rhizophoraceae |
description |
Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii . The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species. Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii . The microsatellite markers presented here will be useful in studies of contemporary and historical gene fl ow between American and West African Rhizophora species. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-09-02 |
dc.date.accessioned.fl_str_mv |
2017-11-01T10:40:18Z |
dc.date.available.fl_str_mv |
2017-11-01T10:40:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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21680450 |
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21680450 |
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https://doi.org/10.3732/apps.1300003 http://www.locus.ufv.br/handle/123456789/12673 |
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eng |
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eng |
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1(9): 1300003, September 2013 |
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