Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental

Detalhes bibliográficos
Autor(a) principal: Lavorato, Victor Neiva
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/3502
Resumo: The aim of this study was to evaluate the effects of low-intensity aerobic exercise training associated with mesenchymal stem cell therapy (MSCs) on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction (MI). Male Wistar rats (age: 30 days; body weight: 118 ± 11g) were divided into six groups: control sham (CON SH), trained sham (TR SH), control infarction (CON MI), trained infarction (TR MI), control infarction plus stem cells (CON MI SC) and trained infarction plus stem cells (TR MI SC). Animals from MI groups were subjected to throcotomy ligation of the anterior descending coronary artery. Animals from Sham groups were submitted to the same procedure but ligation of the coronary branch. Animals from TR groups were submitted to a progressive treadmill running training for 12 weeks, being the final duration and intensity of 60 min and 60-70% of maximal running speed, respectively. MSCs from the Wistar rat femoral bone marrow were used to allogenic cell transplantation through the tail vein (concentration: 1 x 106 cells). Hemodynamic analyzes were performed by echocardiography at the end of the experimental period. The infarct size was determined by histomorphometry. The mechanical properties and the intracellular global calcium transient [Ca2+]i were measured using an edge detection system and confocal microscopy, respectively. The expression of calcium regulatory proteins was measured by western blotting. The results showed that the average infarct size was of 30%. Exercise training increased the total time until fatigue in trained animals as compared to their controls. MI decreased the systolic interventricular septum thickness (ESSIV), and the fractions of ejection and shortening of the left ventricle (LV), with increases in the LV diastolic diameter and in the LV systolic diameter (DSVE). At the cellular level, MI increased the amplitude of cell contraction and of [Ca2+]i transient and the time to 50% decay of [Ca2+]i transient. The cellular therapy increased the fractions of ejection and shortening of the LV and reduced the amplitude and the time to 50% decay of the [Ca2+]i transient. Exercise training reduced the DSVE and increased the fractions of ejection and shortening of the LV, the LV thickness and the ESSIV. At the cellular level, exercise training reduced the time to peak of contraction and the time to 50% relaxation and increased the amplitude of the [Ca2+]i transient. It also augmented the expression of the Ca2+ ATPase of the sarcoplasmic reticulum (SERCA 2a) and phospholamban phosphorylated at serine 16 (PLBser16). The combination of exercise training and cellular therapy decreased the time to peak of contraction and increased in the amplitude of the [Ca2+]i transient, as well as the expression of PLBser16. In conclusion, low-intensity exercise training and therapy with MSCs, either isolated or in combination, promotes beneficial adaptations to the heart of rats with experimental MI.
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spelling Lavorato, Victor Neivahttp://lattes.cnpq.br/4543625551897114Cunha, Daise Nunes Queiroz dahttp://lattes.cnpq.br/1589540442087805Carlo, Ricardo Junqueira Delhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788143A9Natali, Antônio Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795725H4Oliveira, Edilamar Menezes dehttp://lattes.cnpq.br/07647291785203492015-03-26T13:22:05Z2015-01-222015-03-26T13:22:05Z2014-03-28LAVORATO, Victor Neiva. Effects of aerobic exercise training associated with mesenchymal stem cell therapy on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction. 2014. 86 f. Dissertação (Mestrado em Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/3502The aim of this study was to evaluate the effects of low-intensity aerobic exercise training associated with mesenchymal stem cell therapy (MSCs) on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction (MI). Male Wistar rats (age: 30 days; body weight: 118 ± 11g) were divided into six groups: control sham (CON SH), trained sham (TR SH), control infarction (CON MI), trained infarction (TR MI), control infarction plus stem cells (CON MI SC) and trained infarction plus stem cells (TR MI SC). Animals from MI groups were subjected to throcotomy ligation of the anterior descending coronary artery. Animals from Sham groups were submitted to the same procedure but ligation of the coronary branch. Animals from TR groups were submitted to a progressive treadmill running training for 12 weeks, being the final duration and intensity of 60 min and 60-70% of maximal running speed, respectively. MSCs from the Wistar rat femoral bone marrow were used to allogenic cell transplantation through the tail vein (concentration: 1 x 106 cells). Hemodynamic analyzes were performed by echocardiography at the end of the experimental period. The infarct size was determined by histomorphometry. The mechanical properties and the intracellular global calcium transient [Ca2+]i were measured using an edge detection system and confocal microscopy, respectively. The expression of calcium regulatory proteins was measured by western blotting. The results showed that the average infarct size was of 30%. Exercise training increased the total time until fatigue in trained animals as compared to their controls. MI decreased the systolic interventricular septum thickness (ESSIV), and the fractions of ejection and shortening of the left ventricle (LV), with increases in the LV diastolic diameter and in the LV systolic diameter (DSVE). At the cellular level, MI increased the amplitude of cell contraction and of [Ca2+]i transient and the time to 50% decay of [Ca2+]i transient. The cellular therapy increased the fractions of ejection and shortening of the LV and reduced the amplitude and the time to 50% decay of the [Ca2+]i transient. Exercise training reduced the DSVE and increased the fractions of ejection and shortening of the LV, the LV thickness and the ESSIV. At the cellular level, exercise training reduced the time to peak of contraction and the time to 50% relaxation and increased the amplitude of the [Ca2+]i transient. It also augmented the expression of the Ca2+ ATPase of the sarcoplasmic reticulum (SERCA 2a) and phospholamban phosphorylated at serine 16 (PLBser16). The combination of exercise training and cellular therapy decreased the time to peak of contraction and increased in the amplitude of the [Ca2+]i transient, as well as the expression of PLBser16. In conclusion, low-intensity exercise training and therapy with MSCs, either isolated or in combination, promotes beneficial adaptations to the heart of rats with experimental MI.O objetivo deste estudo foi avaliar os efeitos do treinamento aeróbio de baixa intensidade associado à terapia de células-tronco mesenquimais (CTMs) nos parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio (IM) experimental. Ratos Wistar (idade: 30 dias; peso corporal: 118,01 ± 11,24g) foram divididos em seis grupos experimentais: CON SH e TR SH Controle Sham e Treinado Sham; CON IM e TR IM Controle Infartado e Treinado Infartado; CON IM CT e TR IM CT Controle Infartado + CTMs e Treinado Infartado + CTMs. Os animais dos grupos IM foram submetidos à toracotomia e ligadura da artéria coronária anterior descendente. Os animais dos grupos Sham foram submetidos ao mesmo procedimento, mas sem a ligadura. Os animais dos grupos TR foram submetidos a um programa progressivo de corrida em esteira por 12 semanas, sendo a duração e a intensidade final de 60 minutos e 60-70% da velocidade máxima de corrida, respectivamente. CTMs da medula óssea do fêmur de ratos Wistar foram usadas para transplante celular alogênico através da veia caudal (concentração: 1 x 106 células). As análises hemodinâmicas foram realizadas por ecocardiografia, ao final do experimento. O tamanho do infarto foi determinado por histomorfometria. As propriedades mecânicas e o transiente intracellular global de cálcio ([Ca2+]i) de cardiomiócitos da área remanescente ao IM foram mensurados usando-se um sistema de detecção de bordas e microscopia confocal, respectivamente. A expressão das proteínas reguladoras do transiente de [Ca2+]i foi determinada por Western Blot. Os resultados mostraram que o tamanho do infarto foi em média 30%. As CTMs foram localizadas apenas no pulmão dos animais. O treinamento aplicado aumentou o tempo total até a fadiga nos animais treinados, comparados aos controles sedentários. O infarto diminuiu a espessura do septo interventricular (ESSIV), as frações de ejeção e de encurtamento do ventrículo esquerdo (VE), com aumentos no diâmetro diastólico e sistólico (DSVE) do VE. Em nível celular, o IM aumentou a amplitude de contração e do transiente de [Ca2+]i, e o tempo para 50% do decaimento de [Ca2+]i. A terapia com CTMs aumentou as frações de ejeção e de encurtamento do VE e reduziu a amplitude e o tempo para 50% do decaimento de [Ca2+]i. O treinamento aplicado diminuiu o DSVE e aumentou as frações de ejeção e de encurtamento do VE, a espessura do VE e a ESSIV. Além disso, reduziu o tempo para o pico de contração e o tempo para 50% do relaxamento e aumentou a amplitude do transiente de [Ca2+]i, assim como a expressão da Ca2+ ATPase do retículo sarcoplasmático e da fosfolambam fosforilada na serina 16 (FLBser16). A associação do treinamento físico à terapia com CTMs reduziu o tempo para o pico de contração e aumentou a amplitude de [Ca2+]i, bem como a expressão de FLBser16. Conclui-se que o treinamento físico aeróbio de baixa intensidade e a terapia com CTMs, isoladamente ou em associação, promovem adaptações benéficas em parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com IM experimental.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Educação FísicaUFVBRAspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humanoInfarto do miocárdioTreinamento físicoExercícios aeróbicosRatos wistarCélulas-troncoMyocardial infarctionPhysical trainingAerobic exercisesWistar ratsStem cellsCNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICAEfeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimentalEffects of aerobic exercise training associated with mesenchymal stem cell therapy on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarctioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1639656https://locus.ufv.br//bitstream/123456789/3502/1/texto%20completo.pdf0bea32384b91b19501d4bca7501e754cMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain159471https://locus.ufv.br//bitstream/123456789/3502/2/texto%20completo.pdf.txt2681aea7cf7cb3c1a30f28592d6b2ebcMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3627https://locus.ufv.br//bitstream/123456789/3502/3/texto%20completo.pdf.jpg01426d244ba112e9761e2a9005aff76bMD53123456789/35022016-04-09 23:09:26.816oai:locus.ufv.br:123456789/3502Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-10T02:09:26LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
dc.title.alternative.eng.fl_str_mv Effects of aerobic exercise training associated with mesenchymal stem cell therapy on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction
title Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
spellingShingle Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
Lavorato, Victor Neiva
Infarto do miocárdio
Treinamento físico
Exercícios aeróbicos
Ratos wistar
Células-tronco
Myocardial infarction
Physical training
Aerobic exercises
Wistar rats
Stem cells
CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
title_short Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
title_full Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
title_fullStr Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
title_full_unstemmed Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
title_sort Efeitos do treinamento físico aeróbio associado à terapia com células-tronco mesenquimais sobre parâmetros cardiovasculares e propriedades mecânicas de cardiomiócitos em ratos com infarto do miocárdio experimental
author Lavorato, Victor Neiva
author_facet Lavorato, Victor Neiva
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/4543625551897114
dc.contributor.author.fl_str_mv Lavorato, Victor Neiva
dc.contributor.advisor-co1.fl_str_mv Cunha, Daise Nunes Queiroz da
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1589540442087805
dc.contributor.advisor-co2.fl_str_mv Carlo, Ricardo Junqueira Del
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788143A9
dc.contributor.advisor1.fl_str_mv Natali, Antônio José
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795725H4
dc.contributor.referee1.fl_str_mv Oliveira, Edilamar Menezes de
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/0764729178520349
contributor_str_mv Cunha, Daise Nunes Queiroz da
Carlo, Ricardo Junqueira Del
Natali, Antônio José
Oliveira, Edilamar Menezes de
dc.subject.por.fl_str_mv Infarto do miocárdio
Treinamento físico
Exercícios aeróbicos
Ratos wistar
Células-tronco
topic Infarto do miocárdio
Treinamento físico
Exercícios aeróbicos
Ratos wistar
Células-tronco
Myocardial infarction
Physical training
Aerobic exercises
Wistar rats
Stem cells
CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
dc.subject.eng.fl_str_mv Myocardial infarction
Physical training
Aerobic exercises
Wistar rats
Stem cells
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
description The aim of this study was to evaluate the effects of low-intensity aerobic exercise training associated with mesenchymal stem cell therapy (MSCs) on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction (MI). Male Wistar rats (age: 30 days; body weight: 118 ± 11g) were divided into six groups: control sham (CON SH), trained sham (TR SH), control infarction (CON MI), trained infarction (TR MI), control infarction plus stem cells (CON MI SC) and trained infarction plus stem cells (TR MI SC). Animals from MI groups were subjected to throcotomy ligation of the anterior descending coronary artery. Animals from Sham groups were submitted to the same procedure but ligation of the coronary branch. Animals from TR groups were submitted to a progressive treadmill running training for 12 weeks, being the final duration and intensity of 60 min and 60-70% of maximal running speed, respectively. MSCs from the Wistar rat femoral bone marrow were used to allogenic cell transplantation through the tail vein (concentration: 1 x 106 cells). Hemodynamic analyzes were performed by echocardiography at the end of the experimental period. The infarct size was determined by histomorphometry. The mechanical properties and the intracellular global calcium transient [Ca2+]i were measured using an edge detection system and confocal microscopy, respectively. The expression of calcium regulatory proteins was measured by western blotting. The results showed that the average infarct size was of 30%. Exercise training increased the total time until fatigue in trained animals as compared to their controls. MI decreased the systolic interventricular septum thickness (ESSIV), and the fractions of ejection and shortening of the left ventricle (LV), with increases in the LV diastolic diameter and in the LV systolic diameter (DSVE). At the cellular level, MI increased the amplitude of cell contraction and of [Ca2+]i transient and the time to 50% decay of [Ca2+]i transient. The cellular therapy increased the fractions of ejection and shortening of the LV and reduced the amplitude and the time to 50% decay of the [Ca2+]i transient. Exercise training reduced the DSVE and increased the fractions of ejection and shortening of the LV, the LV thickness and the ESSIV. At the cellular level, exercise training reduced the time to peak of contraction and the time to 50% relaxation and increased the amplitude of the [Ca2+]i transient. It also augmented the expression of the Ca2+ ATPase of the sarcoplasmic reticulum (SERCA 2a) and phospholamban phosphorylated at serine 16 (PLBser16). The combination of exercise training and cellular therapy decreased the time to peak of contraction and increased in the amplitude of the [Ca2+]i transient, as well as the expression of PLBser16. In conclusion, low-intensity exercise training and therapy with MSCs, either isolated or in combination, promotes beneficial adaptations to the heart of rats with experimental MI.
publishDate 2014
dc.date.issued.fl_str_mv 2014-03-28
dc.date.accessioned.fl_str_mv 2015-03-26T13:22:05Z
dc.date.available.fl_str_mv 2015-01-22
2015-03-26T13:22:05Z
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dc.identifier.citation.fl_str_mv LAVORATO, Victor Neiva. Effects of aerobic exercise training associated with mesenchymal stem cell therapy on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction. 2014. 86 f. Dissertação (Mestrado em Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/3502
identifier_str_mv LAVORATO, Victor Neiva. Effects of aerobic exercise training associated with mesenchymal stem cell therapy on cardiovascular parameters and mechanical properties of cardiomyocytes in rats with experimental myocardial infarction. 2014. 86 f. Dissertação (Mestrado em Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano) - Universidade Federal de Viçosa, Viçosa, 2014.
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dc.publisher.department.fl_str_mv Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano
publisher.none.fl_str_mv Universidade Federal de Viçosa
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