Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse

Detalhes bibliográficos
Autor(a) principal: Pires, Raquel Maria de Oliveira
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4632
Resumo: Seeds frequently encounter biotic and abiotic stress that adversely affects growth, development, or productivity. The objective of this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different stresses. Therefore, two experiments were conducted. In the first one, sesame seeds were placed in soaked substrate with water or with different concentrations of cadmium chloride and cadmium chloride plus sodium nitroprusside (SNP) in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 800 μM of CdCl2, 4) 800 μM of CdCl2 +200 μM of SNP, 5) 600 μM of CdCl2, 6) 600 μM of CdCl2 +200 μM of SNP, 7) 400 μM of CdCl2 and 8) 400 μM of CdCl2 +200 μM of SNP. Were done the following determinations: germination (G), first count of germination (PC), speed germination index (IVG), hipocotyl length (CH), radicle length (CR), dry mass of hipocotyl (MSH) and dry mass of radicle (DMR) and imbibition curve. It were quantified the activity of antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and total peroxidase (POX). It was adopted the completely randomized design with five replicates. The data were submitted to variance analysis and the means obtained for treatments were compared by Tukey test at 5% of significance. The means obtained in treatments with and without SNP were compared by F test at 5% and the times of imbibition by Tukey test at 5%. For the realization of imbibition curve was realized a polynomial regression fit 3. In the second experiment, sesame seeds were placed in soaked substrate with water or with different concentrations of PEG 6000 and PEG 6000 plus SNP in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 0,1MPa, 4) -0,1MPa +200 μM of SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM of SNP, 7) -0,3MPa and 8) -0,3MPa, +200 μM of SNP. The same determinations done in experiment one, was made in the experiment two. Both stress caused the reduction of germination and vigor, being the highest concentrations, the most harmful to sesame seeds. NO was able to minimize the damage caused by the stresses, reversing partially all the biochemical parameters evaluated, what provides higher and faster germination, beyond the higher initial development of xiii seedlings. Cadmium and PEG 6000 provoked less absorption and less gain of mass in higher concentrations in relation to water, and an extension of Phase II. In relation to the antioxydative system, there was an increaseof enzymatic activity in period of 0 to 24 houras, showing the organization of the antioxidant system with the sesame seeds over time. Was observed that in higher potentials and in solutions more concentrated, the activity enzymatic was higher, what suggest detoxifying activity of these molecules in seeds submitted to water deficit and solution of cadmium more strict. The use of SNP, increased the activity of antioxidative enzymes, evidencing an efficient of elimination of EROs formed.
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spelling Pires, Raquel Maria de Oliveirahttp://lattes.cnpq.br/8684079739368288Borges, Eduardo Euclydes de Lima ehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787799U8Dias, Denise Cunha Fernandes dos Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727304Z9Dias, Luiz Antonio dos Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763137P6Ataíde, Glauciana da Matahttp://lattes.cnpq.br/80010320105194062015-03-26T13:40:03Z2014-11-072015-03-26T13:40:03Z2014-02-26PIRES, Raquel Maria de Oliveira. Protective action of nitric oxide on sesame seeds (Sesamum indicum L.) submitted to different conditions of stresse. 2014. 86 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/4632Seeds frequently encounter biotic and abiotic stress that adversely affects growth, development, or productivity. The objective of this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different stresses. Therefore, two experiments were conducted. In the first one, sesame seeds were placed in soaked substrate with water or with different concentrations of cadmium chloride and cadmium chloride plus sodium nitroprusside (SNP) in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 800 μM of CdCl2, 4) 800 μM of CdCl2 +200 μM of SNP, 5) 600 μM of CdCl2, 6) 600 μM of CdCl2 +200 μM of SNP, 7) 400 μM of CdCl2 and 8) 400 μM of CdCl2 +200 μM of SNP. Were done the following determinations: germination (G), first count of germination (PC), speed germination index (IVG), hipocotyl length (CH), radicle length (CR), dry mass of hipocotyl (MSH) and dry mass of radicle (DMR) and imbibition curve. It were quantified the activity of antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and total peroxidase (POX). It was adopted the completely randomized design with five replicates. The data were submitted to variance analysis and the means obtained for treatments were compared by Tukey test at 5% of significance. The means obtained in treatments with and without SNP were compared by F test at 5% and the times of imbibition by Tukey test at 5%. For the realization of imbibition curve was realized a polynomial regression fit 3. In the second experiment, sesame seeds were placed in soaked substrate with water or with different concentrations of PEG 6000 and PEG 6000 plus SNP in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 0,1MPa, 4) -0,1MPa +200 μM of SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM of SNP, 7) -0,3MPa and 8) -0,3MPa, +200 μM of SNP. The same determinations done in experiment one, was made in the experiment two. Both stress caused the reduction of germination and vigor, being the highest concentrations, the most harmful to sesame seeds. NO was able to minimize the damage caused by the stresses, reversing partially all the biochemical parameters evaluated, what provides higher and faster germination, beyond the higher initial development of xiii seedlings. Cadmium and PEG 6000 provoked less absorption and less gain of mass in higher concentrations in relation to water, and an extension of Phase II. In relation to the antioxydative system, there was an increaseof enzymatic activity in period of 0 to 24 houras, showing the organization of the antioxidant system with the sesame seeds over time. Was observed that in higher potentials and in solutions more concentrated, the activity enzymatic was higher, what suggest detoxifying activity of these molecules in seeds submitted to water deficit and solution of cadmium more strict. The use of SNP, increased the activity of antioxidative enzymes, evidencing an efficient of elimination of EROs formed.Sementes estão frequentemente expostas a diversos estresses bióticos e abióticos que prejudicam o seu crescimento, desenvolvimento e produtividade. O objetivo desse trabalho foi investigar o efeito do óxido nítrico (ON) como agente protetor em sementes de gergelim (Sesamum indicum L.) submetidas à diferentes estresses. Para tanto, foram conduzidos dois experimentos. No primeiro, as sementes foram semeadas em substrato umedecido com água ou com diferentes concentrações de cloreto de cádmio (CdCl2) e cloreto de cádmio acrescido de nitroprussiato de sódio (SNP) nos seguintes tratamentos: 1) água (controle), 2) +200 μM de SNP, 3) 800 μM de CdCl2, 4) 800 μM de CdCl2 +200 μM de SNP, 5) 600 μM de CdCl2, 6) 600 μM de CdCl2 +200 μM de SNP, 7) 400 μM de CdCl2 e 8) 400 μM de CdCl2 +200 μM de SNP. As seguintes determinações foram feitas: germinação (G), primeira contagem de germinação (PC), índice de velocidade de germinação (IVG), comprimento de hipocótilo (CH) e radícula (CR), massa seca de hipocótilo (MSH) e radícula (MSR), curva de embebiçao, além da quantificação da atividade das enzimas antioxidativas, superóxido dismutase (SOD), catalase (CAT), ascorbato peroxidase (APX) e peroxidases totais (POX). O delineamento estatístico utilizado foi o inteiramente casualizado com cinco repetições. Os dados foram submetidos à análise de variância (ANOVA) e as médias obtidas para os tratamentos foram comparadas pelo teste Tukey a 5% de significância. As médias obtidas nos tratamentos com e sem SNP foram comparadas pelo teste F a 5% de significância e os tempos de embebição na análise das atividades das enzimas, também pelo teste Tukey a 5%. Para a realização da curva de embebição realizou-se regressão com ajuste polinomial de grau 3. No segundo experimento, as sementes de gergelim foram semeadas em substrato umedecido com água ou em diferentes concentrações de polietileno glicol (PEG 6000) e PEG 6000 acrescido de SNP nos seguintes tratamentos: 1) água (controle), 2) +200 μM de SNP, 3) 0,1Mpa, 4) -0,1MPa +200 μM de SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM de SNP, -7) 0,3MPa e 8) -0,3MPa, +200 μM de SNP. As mesmas determinações e análises estatísticas do primeiro experimento foram realizadas. Conclui-se que ambos os estresses causaram a redução da germinação e do vigor, sendo Xi as maiores concentrações as mais prejudiciais às sementes de gergelim. O ON foi capaz de amenizar os danos causados pelos estresses invertendo parcialmente todos os parâmetros fisiológicos avaliados, o que proporcionou maior e mais rápida germinação além de maior desenvolvimento inicial das plântulas. O cádmio e o PEG 6000 provocaram menor absorção e menor ganho de massa nas maiores concentrações em relação à água assim como um prolongamento da Fase II da embebição. Em relação ao sistema antioxidante, houve aumento da atividade enzimática no período de 0 a 24 horas, demonstrando organização do sistema antioxidante nas sementes de gergelim com o decorrer do tempo. Observou-se que nos maiores potenciais e nas soluções mais concentradas, a atividade enzimática foi maior, o que sugere atividade desintoxicante dessas moléculas em sementes submetidas ao déficit hídrico e solução de cádmio mais rigorosa. O uso do SNP, de maneira geral, aumentou a atividade das enzimas antioxidantes, evidenciando um eficiente sistema de eliminação das espécies reativas de oxigênio formadas.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em FitotecniaUFVBRPlantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita deGergelimSesamum indicum L.SementesGerminaçãoCádmioNitroprussiato de Sódio (SNP)SesameSesamum indicum L .SeedsGerminationCadmiumSodium nitroprusside (SNP)CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAAção protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresseProtective action of nitric oxide on sesame seeds (Sesamum indicum L.) submitted to different conditions of stresseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf532553https://locus.ufv.br//bitstream/123456789/4632/1/texto%20completo.pdfafb4fdf0d2eec084cc5b732167b89c7dMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain143309https://locus.ufv.br//bitstream/123456789/4632/2/texto%20completo.pdf.txtf9533a88ec579b25c74b1195efc2a8d1MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3557https://locus.ufv.br//bitstream/123456789/4632/3/texto%20completo.pdf.jpg83e1681b5b0daab6c335872b2c2c9836MD53123456789/46322016-04-10 23:11:35.828oai:locus.ufv.br:123456789/4632Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:11:35LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
dc.title.alternative.eng.fl_str_mv Protective action of nitric oxide on sesame seeds (Sesamum indicum L.) submitted to different conditions of stresse
title Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
spellingShingle Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
Pires, Raquel Maria de Oliveira
Gergelim
Sesamum indicum L.
Sementes
Germinação
Cádmio
Nitroprussiato de Sódio (SNP)
Sesame
Sesamum indicum L .
Seeds
Germination
Cadmium
Sodium nitroprusside (SNP)
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
title_short Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
title_full Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
title_fullStr Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
title_full_unstemmed Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
title_sort Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse
author Pires, Raquel Maria de Oliveira
author_facet Pires, Raquel Maria de Oliveira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/8684079739368288
dc.contributor.author.fl_str_mv Pires, Raquel Maria de Oliveira
dc.contributor.advisor-co1.fl_str_mv Borges, Eduardo Euclydes de Lima e
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787799U8
dc.contributor.advisor1.fl_str_mv Dias, Denise Cunha Fernandes dos Santos
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727304Z9
dc.contributor.referee1.fl_str_mv Dias, Luiz Antonio dos Santos
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763137P6
dc.contributor.referee2.fl_str_mv Ataíde, Glauciana da Mata
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8001032010519406
contributor_str_mv Borges, Eduardo Euclydes de Lima e
Dias, Denise Cunha Fernandes dos Santos
Dias, Luiz Antonio dos Santos
Ataíde, Glauciana da Mata
dc.subject.por.fl_str_mv Gergelim
Sesamum indicum L.
Sementes
Germinação
Cádmio
Nitroprussiato de Sódio (SNP)
topic Gergelim
Sesamum indicum L.
Sementes
Germinação
Cádmio
Nitroprussiato de Sódio (SNP)
Sesame
Sesamum indicum L .
Seeds
Germination
Cadmium
Sodium nitroprusside (SNP)
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
dc.subject.eng.fl_str_mv Sesame
Sesamum indicum L .
Seeds
Germination
Cadmium
Sodium nitroprusside (SNP)
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
description Seeds frequently encounter biotic and abiotic stress that adversely affects growth, development, or productivity. The objective of this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different stresses. Therefore, two experiments were conducted. In the first one, sesame seeds were placed in soaked substrate with water or with different concentrations of cadmium chloride and cadmium chloride plus sodium nitroprusside (SNP) in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 800 μM of CdCl2, 4) 800 μM of CdCl2 +200 μM of SNP, 5) 600 μM of CdCl2, 6) 600 μM of CdCl2 +200 μM of SNP, 7) 400 μM of CdCl2 and 8) 400 μM of CdCl2 +200 μM of SNP. Were done the following determinations: germination (G), first count of germination (PC), speed germination index (IVG), hipocotyl length (CH), radicle length (CR), dry mass of hipocotyl (MSH) and dry mass of radicle (DMR) and imbibition curve. It were quantified the activity of antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and total peroxidase (POX). It was adopted the completely randomized design with five replicates. The data were submitted to variance analysis and the means obtained for treatments were compared by Tukey test at 5% of significance. The means obtained in treatments with and without SNP were compared by F test at 5% and the times of imbibition by Tukey test at 5%. For the realization of imbibition curve was realized a polynomial regression fit 3. In the second experiment, sesame seeds were placed in soaked substrate with water or with different concentrations of PEG 6000 and PEG 6000 plus SNP in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 0,1MPa, 4) -0,1MPa +200 μM of SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM of SNP, 7) -0,3MPa and 8) -0,3MPa, +200 μM of SNP. The same determinations done in experiment one, was made in the experiment two. Both stress caused the reduction of germination and vigor, being the highest concentrations, the most harmful to sesame seeds. NO was able to minimize the damage caused by the stresses, reversing partially all the biochemical parameters evaluated, what provides higher and faster germination, beyond the higher initial development of xiii seedlings. Cadmium and PEG 6000 provoked less absorption and less gain of mass in higher concentrations in relation to water, and an extension of Phase II. In relation to the antioxydative system, there was an increaseof enzymatic activity in period of 0 to 24 houras, showing the organization of the antioxidant system with the sesame seeds over time. Was observed that in higher potentials and in solutions more concentrated, the activity enzymatic was higher, what suggest detoxifying activity of these molecules in seeds submitted to water deficit and solution of cadmium more strict. The use of SNP, increased the activity of antioxidative enzymes, evidencing an efficient of elimination of EROs formed.
publishDate 2014
dc.date.available.fl_str_mv 2014-11-07
2015-03-26T13:40:03Z
dc.date.issued.fl_str_mv 2014-02-26
dc.date.accessioned.fl_str_mv 2015-03-26T13:40:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv PIRES, Raquel Maria de Oliveira. Protective action of nitric oxide on sesame seeds (Sesamum indicum L.) submitted to different conditions of stresse. 2014. 86 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4632
identifier_str_mv PIRES, Raquel Maria de Oliveira. Protective action of nitric oxide on sesame seeds (Sesamum indicum L.) submitted to different conditions of stresse. 2014. 86 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2014.
url http://locus.ufv.br/handle/123456789/4632
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dc.publisher.program.fl_str_mv Mestrado em Fitotecnia
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de
publisher.none.fl_str_mv Universidade Federal de Viçosa
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