Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)

Detalhes bibliográficos
Autor(a) principal: Carvalho, Mychelle
Data de Publicação: 2009
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1131
Resumo: The study aimed to establish an efficient protocol for somatic embryogenesis of the oil palm, especially all of cultivars explored in Brazil, as well as to acomplish the anatomical caracterization of the embryogenic process. For induction of somatic embryogenesis we used two explants types, leaves segments and female inflorescences, resulting in two experiments. In the first experiment, sections of the 5 mm in immature leaves from two oil palm genotypes (G1001 e G2301) of 1,5 years of age were inoculated in culture medium Y3 (Eeuwens, 1978) supplemented with 0.3% activated charcoal and 2,4-D at four concentrations (800, 1000, 1200 and 1400 µM). Higher percentage of callus induction was obtained in G2301 leaf explants submitted to the concentration 800 µM of 2,4-D. The obtaining of pro-embryogenic masses starting of the calli occurred in medium supplemented with 9 µM 2,4-D added to 1000 µM putrescine or 100 µM spermine. A higher percentage of regeneration of somatic embryos occurred from pro-embryogenic masses that were pre-conditioned and inoculated on regeneration medium supplemented with 0.045 µM 2,4-D and 1000 µM putrescine. The somatic embryos regenerated exhibited characteristic protoderm, procambium strands and shoot meristem and germinated in culture medium Y3 added 0.54 µM ANA and 1000 µM putrescine. The seedlings showed simultaneous development of shoot and root and 70.4% of plants could be successfully acclimatized. In the second experiment, rachillas taken from immature female inflorescences in two developmental stages were inoculated in different culture media (MS and Y3), the supplemented with 475 µM of different auxins (Picloram and 2,4-D) and 0.3% activated charcoal . The development of flowers occurred after 120 days of inoculation in vitro, depending on the developmental stage of the inflorescence and the growing medium. The flowers formed in Y3 culture medium were detached from rachillas and inoculated in the same culture medium with reduced auxin concentrations for 9 µM being combined or not with 1000 µM of putrescine. After 60 days, greater callus formation occurred in flowers grown in medium supplemented with 9 µM Picloram combined with 1000 µM of putrescine. The regeneration of somatic embryos occurred after reduction at 100 times the concentration of auxin in the culture medium, maintaining the same concentration of putrescine. Embryos showed protoderm well defined, delimiting a homogeneous mass of cells corresponding to the ground meristem, interspersed with well-defined procambium strands. By means of histological analysis it was found that the formation occurred from the perivascular regions in the gynoecium and embryo formation occurred from the regions of meristematic callus, following the multicellular pattern.
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spelling Carvalho, Mychellehttp://lattes.cnpq.br/6211400266640051Ventrella, Marília Continhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763436A2Otoni, Wagner Camposhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y6Motoike, Sérgio Yoshimitsuhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728221T8Sato, Aurora Yoshikohttp://lattes.cnpq.br/5031864568031327Paes, José Mauro Valente2015-03-26T12:43:36Z2011-02-222015-03-26T12:43:36Z2009-11-13CARVALHO, Mychelle. Somatic embryogenesis from immature leaves and flowers developed in vitro from oil palm (Elaeis guineensis Jacq). 2009. 86 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/1131The study aimed to establish an efficient protocol for somatic embryogenesis of the oil palm, especially all of cultivars explored in Brazil, as well as to acomplish the anatomical caracterization of the embryogenic process. For induction of somatic embryogenesis we used two explants types, leaves segments and female inflorescences, resulting in two experiments. In the first experiment, sections of the 5 mm in immature leaves from two oil palm genotypes (G1001 e G2301) of 1,5 years of age were inoculated in culture medium Y3 (Eeuwens, 1978) supplemented with 0.3% activated charcoal and 2,4-D at four concentrations (800, 1000, 1200 and 1400 µM). Higher percentage of callus induction was obtained in G2301 leaf explants submitted to the concentration 800 µM of 2,4-D. The obtaining of pro-embryogenic masses starting of the calli occurred in medium supplemented with 9 µM 2,4-D added to 1000 µM putrescine or 100 µM spermine. A higher percentage of regeneration of somatic embryos occurred from pro-embryogenic masses that were pre-conditioned and inoculated on regeneration medium supplemented with 0.045 µM 2,4-D and 1000 µM putrescine. The somatic embryos regenerated exhibited characteristic protoderm, procambium strands and shoot meristem and germinated in culture medium Y3 added 0.54 µM ANA and 1000 µM putrescine. The seedlings showed simultaneous development of shoot and root and 70.4% of plants could be successfully acclimatized. In the second experiment, rachillas taken from immature female inflorescences in two developmental stages were inoculated in different culture media (MS and Y3), the supplemented with 475 µM of different auxins (Picloram and 2,4-D) and 0.3% activated charcoal . The development of flowers occurred after 120 days of inoculation in vitro, depending on the developmental stage of the inflorescence and the growing medium. The flowers formed in Y3 culture medium were detached from rachillas and inoculated in the same culture medium with reduced auxin concentrations for 9 µM being combined or not with 1000 µM of putrescine. After 60 days, greater callus formation occurred in flowers grown in medium supplemented with 9 µM Picloram combined with 1000 µM of putrescine. The regeneration of somatic embryos occurred after reduction at 100 times the concentration of auxin in the culture medium, maintaining the same concentration of putrescine. Embryos showed protoderm well defined, delimiting a homogeneous mass of cells corresponding to the ground meristem, interspersed with well-defined procambium strands. By means of histological analysis it was found that the formation occurred from the perivascular regions in the gynoecium and embryo formation occurred from the regions of meristematic callus, following the multicellular pattern.O trabalho teve como objetivos estabelecer um protocolo eficiente para embriogênese somática do dendezeiro, sobretudo de cultivares exploradas no Brasil, bem como realizar a caracterização anatômica do processo embriogênico. Para a indução da embriogênese somática utilizou-se dois tipos de explantes, segmentos foliares e inflorescências femininas, resultando em dois experimentos. No primeiro experimento, secções de 5 mm de folhas imaturas retiradas de dois genótipos (G1001 e G2301) de dendê de 1,5 ano de idade foram inoculadas em meio de cultura Y3 (Eeuwens, 1978), adicionado de 0,3% de carvão ativado e quatro concentrações de 2,4-D (800, 1000, 1200 e 1400 µM). Maior porcentagem de indução de calos foi obtida em explantes foliares do G2301 submetidos à concentração 800 µM de 2,4-D. A obtenção de massas pró-embriogênicas a partir dos calos ocorreu em meio suplementado com 9 µM de 2,4-D adicionado de 1000 µM putrescina ou 100 µM espermina. Maior porcentagem de regeneração de embriões somáticos ocorreu a partir de massas pró-embriogênicas que passaram pelo pré- condicionamento e foram inoculadas em meio de regeneração adicionado de 0,045 µM 2,4-D e 1000 µM putrescina. Os embriões somáticos regenerados exibiram protoderme característica, cordões de procâmbio e meristema apical e germinaram em meio de cultura Y3 adicionado de 0,54 µM ANA e 1000 µM putrescina. As plântulas obtidas apresentaram desenvolvimento simultâneo de parte aérea e radícula, e puderam ser aclimatizadas, sendo de 70,4% a porcentagem de plantas obtidas. No segundo experimento, ráquilas retiradas de inflorescências femininas imaturas, em dois estágios de desenvolvimento foram inoculadas em diferentes meios de cultivo (MS e Y3), adicionado de 475 µM de diferentes auxinas (Picloram e 2,4-D) e 0,3% de carvão ativado. O desenvolvimento das flores ocorreu após 120 dias da inoculação in vitro, em função do estágio de desenvolvimento da inflorescência e do meio de cultivo utilizado. As flores formadas em meio de cultivo Y3 foram destacadas das ráquilas e inoculadas em mesmo meio de cultivo com redução da concentração das auxinas para 9 µM sendo combinadas ou não com 1000 µM de putrescina. Após 60 dias, maior formação de calos ocorreu em flores cultivadas em meio adicionado de 9 µM Picloram combinado com 1000 µM de putrescina. A regeneração dos embriões somáticos ocorreu após a redução em 100 vezes na concentração de auxina no meio de cultivo, sendo mantida a mesma concentração de putrescina. Os embriões apresentaram protoderme bem definida, delimitando uma massa homogênea de células correspondentes ao meristema fundamental, entremeada por cordões procambiais bem definidos. Por meio da análise histológica verificou-se que a formação de calos ocorreu a partir das regiões perivasculares no gineceu e a formação dos embriões ocorreu a partir das regiões meristemáticas destes calos, seguindo o padrão multicelular.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitotecniaUFVBRPlantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita deEmbriogênese somáticaElaeis guineensisAnatomiaSomatic embryogenesisElaeis guineensisAnatomyCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAEmbriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)Somatic embryogenesis from immature leaves and flowers developed in vitro from oil palm (Elaeis guineensis Jacq)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2286182https://locus.ufv.br//bitstream/123456789/1131/1/texto%20completo.pdf07252744b8ec020f06d0a67c3c49bc67MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain158573https://locus.ufv.br//bitstream/123456789/1131/2/texto%20completo.pdf.txtb4dce7d6300e4af0dbf8a0bc80763773MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3579https://locus.ufv.br//bitstream/123456789/1131/3/texto%20completo.pdf.jpg50ce40f895ad645dfea63e77e48f39b3MD53123456789/11312016-04-06 23:22:31.681oai:locus.ufv.br:123456789/1131Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:22:31LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
dc.title.alternative.eng.fl_str_mv Somatic embryogenesis from immature leaves and flowers developed in vitro from oil palm (Elaeis guineensis Jacq)
title Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
spellingShingle Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
Carvalho, Mychelle
Embriogênese somática
Elaeis guineensis
Anatomia
Somatic embryogenesis
Elaeis guineensis
Anatomy
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
title_short Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
title_full Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
title_fullStr Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
title_full_unstemmed Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
title_sort Embriogênese somática a partir de folhas imaturas e flores desenvolvidas in vitro de dendezeiro (Elaeis guineensis Jacq)
author Carvalho, Mychelle
author_facet Carvalho, Mychelle
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/6211400266640051
dc.contributor.author.fl_str_mv Carvalho, Mychelle
dc.contributor.advisor-co1.fl_str_mv Ventrella, Marília Contin
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763436A2
dc.contributor.advisor-co2.fl_str_mv Otoni, Wagner Campos
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y6
dc.contributor.advisor1.fl_str_mv Motoike, Sérgio Yoshimitsu
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728221T8
dc.contributor.referee1.fl_str_mv Sato, Aurora Yoshiko
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5031864568031327
dc.contributor.referee2.fl_str_mv Paes, José Mauro Valente
contributor_str_mv Ventrella, Marília Contin
Otoni, Wagner Campos
Motoike, Sérgio Yoshimitsu
Sato, Aurora Yoshiko
Paes, José Mauro Valente
dc.subject.por.fl_str_mv Embriogênese somática
Elaeis guineensis
Anatomia
topic Embriogênese somática
Elaeis guineensis
Anatomia
Somatic embryogenesis
Elaeis guineensis
Anatomy
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
dc.subject.eng.fl_str_mv Somatic embryogenesis
Elaeis guineensis
Anatomy
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
description The study aimed to establish an efficient protocol for somatic embryogenesis of the oil palm, especially all of cultivars explored in Brazil, as well as to acomplish the anatomical caracterization of the embryogenic process. For induction of somatic embryogenesis we used two explants types, leaves segments and female inflorescences, resulting in two experiments. In the first experiment, sections of the 5 mm in immature leaves from two oil palm genotypes (G1001 e G2301) of 1,5 years of age were inoculated in culture medium Y3 (Eeuwens, 1978) supplemented with 0.3% activated charcoal and 2,4-D at four concentrations (800, 1000, 1200 and 1400 µM). Higher percentage of callus induction was obtained in G2301 leaf explants submitted to the concentration 800 µM of 2,4-D. The obtaining of pro-embryogenic masses starting of the calli occurred in medium supplemented with 9 µM 2,4-D added to 1000 µM putrescine or 100 µM spermine. A higher percentage of regeneration of somatic embryos occurred from pro-embryogenic masses that were pre-conditioned and inoculated on regeneration medium supplemented with 0.045 µM 2,4-D and 1000 µM putrescine. The somatic embryos regenerated exhibited characteristic protoderm, procambium strands and shoot meristem and germinated in culture medium Y3 added 0.54 µM ANA and 1000 µM putrescine. The seedlings showed simultaneous development of shoot and root and 70.4% of plants could be successfully acclimatized. In the second experiment, rachillas taken from immature female inflorescences in two developmental stages were inoculated in different culture media (MS and Y3), the supplemented with 475 µM of different auxins (Picloram and 2,4-D) and 0.3% activated charcoal . The development of flowers occurred after 120 days of inoculation in vitro, depending on the developmental stage of the inflorescence and the growing medium. The flowers formed in Y3 culture medium were detached from rachillas and inoculated in the same culture medium with reduced auxin concentrations for 9 µM being combined or not with 1000 µM of putrescine. After 60 days, greater callus formation occurred in flowers grown in medium supplemented with 9 µM Picloram combined with 1000 µM of putrescine. The regeneration of somatic embryos occurred after reduction at 100 times the concentration of auxin in the culture medium, maintaining the same concentration of putrescine. Embryos showed protoderm well defined, delimiting a homogeneous mass of cells corresponding to the ground meristem, interspersed with well-defined procambium strands. By means of histological analysis it was found that the formation occurred from the perivascular regions in the gynoecium and embryo formation occurred from the regions of meristematic callus, following the multicellular pattern.
publishDate 2009
dc.date.issued.fl_str_mv 2009-11-13
dc.date.available.fl_str_mv 2011-02-22
2015-03-26T12:43:36Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:43:36Z
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dc.identifier.citation.fl_str_mv CARVALHO, Mychelle. Somatic embryogenesis from immature leaves and flowers developed in vitro from oil palm (Elaeis guineensis Jacq). 2009. 86 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2009.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1131
identifier_str_mv CARVALHO, Mychelle. Somatic embryogenesis from immature leaves and flowers developed in vitro from oil palm (Elaeis guineensis Jacq). 2009. 86 f. Tese (Doutorado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2009.
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de
publisher.none.fl_str_mv Universidade Federal de Viçosa
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