Componentes químicos e atividade biológica do Veneno de Pachycondyla striata F. Smith (Formicidae: Ponerinae)

• Autor(a) principal: Santos, Pollyanna Pereira
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/932
• topic: Pachycondyla striata - Veneno; Formiga - Veneno; Espectometria de massa; Atividade antimicrobiana; Pachycondyla striata - Poison; Ant - Poison; Mass spectrometry; antimicrobial activity; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::TOXICOLOGIA ANIMAL

The venom produced by ants has been reported as a rich source of bioactive molecules with possible pharmacological importance. The reservoir of the venom gland of Pachycondyla striata was chemically analyzed with two-dimensional electrophoresis, HPLC and GC, followed by mass spectrometry analyses of the type Maldi-TOF-TOF and ESI-TOF-TOF and "de novo" sequencing assisted by software. The venom was composed primarily for proteins, peptides and hydrocarbons. Thirty-eight proteins/peptides and 48 non-protein compounds were found in the venom of P. striata. Whithin the identified proteins there are classic proteins from venom (phospholipase A, hyaluronidase and aminopeptidase N), allergenic proteins (allergens of venom) and peptides with biological activity (buforin, dinoponeratoxin Da-1837, U10-ctenitoxin Pn1a and sarafotoxin). Mainly long chain hydrocarbons (C19 - C36) were found among non-protein compounds. The antimicrobial activity of the venom was tested from venom purified by reversed-phase and ion exchange HPLC. Five fractions derived from the fractionation by reverse phase showed high antimicrobial activity against gram- positive and gram negative bacteria, but only analgesine 2, with reported antimicrobial activity, was identified in one of the fractions. To assess the neurobiological and anticonvulsant activity of the venom, the crude and denatured venom of P. striata in different concentrations (25, 100 and 250 mg / mL) was injected intracerebroventricularly in the mice and the behavior of the mice were evaluated. Significant differences were found in the immobility and exploration behaviors of the mice treated with crude venom concentration of 250 mg/mL. The venom of P. striata was ineffective as an anticonvulsant agent.