Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)

Detalhes bibliográficos
Autor(a) principal: Paiva, Carlos Eduardo Costa
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4617
Resumo: The use of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) based biopesticide has the potential to control Spodoptera frugiperda (Lepidoptera: Noctuidae), but large scale production depends on maximizing virus production from infected larvae. The suitable temperature, time of exposure to the pathogen, the age at which the larvae are inoculated with the virus, the viral solution concentration and isolate used are important for viral replication. The experiments were performed in the Biological Control Laboratory of Insects at Embrapa Maize and Sorghum Research Center (CNPMS), located in Sete Lagoas, Minas Gerais, Brazil. The first study aimed to verify the effect of inoculation and incubation (multiplication) temperatures in viral production and the inoculation period. Cannibalism among the larvae of S. frugiperda was similar (around 4%) in different periods and temperatures baculovirus inoculation. The inoculation period did influence mortality and viral production. The higher mortalities of larvae were achieved at 28 °C, however, the production of polyhedral inclusion bodies (PIB) per larvae were higher at 25 °C, reducing the number of larvae needed for the production of a dose of baculovirus expressed larval equivalent (LE/ha). The total production of polyhedral inoculation at the different temperatures was similar, but the incubation at 25 °C produced a larger amount of virus (24.56 x 109 PIB at 25 °C and 20.81 x 109 PIB at 28 °C). In the second study, the lethal concentrations were determined for larvae SfMNPV six, seven and eight days and the best age to perform the inoculation of SfMNPV order to maximize viral production. The lethal concentration (LC50) ranged from 1.89 x 105 to 5.01 x 106 PIB/mL. The LC50 I6 was higher than the I19 to six days old larvae of similar for seven days and for those less than eight days. Total polyhedra production was higher with eight days old larvae for the isolate I6. The highest yields were obtained in viral concentrations above 1.00 x 107 PIB/mL. The production of PIB/larvae increased when I6 was used in eight days old larvae, which reduces the number of larvae needed to achieve dose of baculovirus (LE/ha). The third study aimed to verify the production parameter which is more correlated with viral production, mass, or the number of dead larvae by SfMNPV and also determine the parameters weight equivalent per hectare (estimated larval weight necessary to achieve a dose of baculovirus, based on the application of 2.00 x 1011 PIB/ha) and larvae dead weight due to SfMNPV infection caused by I6. All correlations were positive and significant for the total weight (r= 0.958, n= 60, P< 0.0001) even when considered in each age or concentration separately. For the number of tracks the correlation was also significant when considering the amount of treatment (r= 0.723, n= 60, P< 0.0001) within each age group and in each concentration was significant only at concentrations of 1.00 x 106 PIB/mL (r= 0.643, n= 12, P< 0.0242) and 1.00 x 108 (r= 0.657, n= 12, P< 0.0202), but the correlations are less strong. Larvae of S. frugiperda, killed by SfMNPV inoculated at eight days old had the highest average mass. The equivalent weight per hectare did differ among different viral concentrations of solutions used. The maximization of viral production baculovirus for control of S. frugiperda should be made with the isolated I6 in larvae eight days old maintained at a temperature of 25 °C and the weight of dead larvae by baculoviruses can be considered a more reliable parameter than the number of larvae to produce a commercial product based SfMNPV in view that shows strong and significant correlation with the total polyhedra production.
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spelling Paiva, Carlos Eduardo Costahttp://lattes.cnpq.br/7544068911776191Valicente, Fernando Hercoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781755J8Serrão, José Eduardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6Zanuncio, José Colahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787556T2Sediyama, Carlos Sigueyukihttp://lattes.cnpq.br/3647241087918239Oliveira, Eugenio Eduardo dehttp://lattes.cnpq.br/00985640669360242015-03-26T13:40:00Z2014-01-272015-03-26T13:40:00Z2013-07-26PAIVA, Carlos Eduardo Costa. Multiplication of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae). 2013. 76 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4617The use of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) based biopesticide has the potential to control Spodoptera frugiperda (Lepidoptera: Noctuidae), but large scale production depends on maximizing virus production from infected larvae. The suitable temperature, time of exposure to the pathogen, the age at which the larvae are inoculated with the virus, the viral solution concentration and isolate used are important for viral replication. The experiments were performed in the Biological Control Laboratory of Insects at Embrapa Maize and Sorghum Research Center (CNPMS), located in Sete Lagoas, Minas Gerais, Brazil. The first study aimed to verify the effect of inoculation and incubation (multiplication) temperatures in viral production and the inoculation period. Cannibalism among the larvae of S. frugiperda was similar (around 4%) in different periods and temperatures baculovirus inoculation. The inoculation period did influence mortality and viral production. The higher mortalities of larvae were achieved at 28 °C, however, the production of polyhedral inclusion bodies (PIB) per larvae were higher at 25 °C, reducing the number of larvae needed for the production of a dose of baculovirus expressed larval equivalent (LE/ha). The total production of polyhedral inoculation at the different temperatures was similar, but the incubation at 25 °C produced a larger amount of virus (24.56 x 109 PIB at 25 °C and 20.81 x 109 PIB at 28 °C). In the second study, the lethal concentrations were determined for larvae SfMNPV six, seven and eight days and the best age to perform the inoculation of SfMNPV order to maximize viral production. The lethal concentration (LC50) ranged from 1.89 x 105 to 5.01 x 106 PIB/mL. The LC50 I6 was higher than the I19 to six days old larvae of similar for seven days and for those less than eight days. Total polyhedra production was higher with eight days old larvae for the isolate I6. The highest yields were obtained in viral concentrations above 1.00 x 107 PIB/mL. The production of PIB/larvae increased when I6 was used in eight days old larvae, which reduces the number of larvae needed to achieve dose of baculovirus (LE/ha). The third study aimed to verify the production parameter which is more correlated with viral production, mass, or the number of dead larvae by SfMNPV and also determine the parameters weight equivalent per hectare (estimated larval weight necessary to achieve a dose of baculovirus, based on the application of 2.00 x 1011 PIB/ha) and larvae dead weight due to SfMNPV infection caused by I6. All correlations were positive and significant for the total weight (r= 0.958, n= 60, P< 0.0001) even when considered in each age or concentration separately. For the number of tracks the correlation was also significant when considering the amount of treatment (r= 0.723, n= 60, P< 0.0001) within each age group and in each concentration was significant only at concentrations of 1.00 x 106 PIB/mL (r= 0.643, n= 12, P< 0.0242) and 1.00 x 108 (r= 0.657, n= 12, P< 0.0202), but the correlations are less strong. Larvae of S. frugiperda, killed by SfMNPV inoculated at eight days old had the highest average mass. The equivalent weight per hectare did differ among different viral concentrations of solutions used. The maximization of viral production baculovirus for control of S. frugiperda should be made with the isolated I6 in larvae eight days old maintained at a temperature of 25 °C and the weight of dead larvae by baculoviruses can be considered a more reliable parameter than the number of larvae to produce a commercial product based SfMNPV in view that shows strong and significant correlation with the total polyhedra production.A utilização de bioinseticida a base de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) tem potencial para o controle de Spodoptera frugiperda (Lepidoptera: Noctuidae), porém seu uso em larga escala depende de se maximizar sua produção a partir de larvas infectadas. A temperatura adequada, o tempo de exposição ao patógeno, a idade na qual as lagartas são inoculadas com o vírus, a concentração da suspensão viral e o isolado são importantes para a replicação viral. Os experimentos foram realizados no Laboratório de Controle Biológico de Insetos do Centro Nacional de Pesquisas com Milho e Sorgo (CNPMS) da Embrapa em Sete Lagoas, Minas Gerais, Brasil. O primeiro estudo objetivou verificar o efeito das temperaturas de inoculação e incubação (multiplicação) na produção viral e do tempo de inoculação. O canibalismo entre as lagartas de S. frugiperda foi semelhante (em torno de 4%) nos diferentes tempos e temperaturas de inoculação do baculovírus. O tempo de inoculação não influenciou a mortalidade e a produção viral. A mortalidade de lagartas foi maior a 28 °C, porém, a produção de corpos poliédricos de inclusão (PIB) por lagarta foi maior a 25 °C, o que reduz o número de lagartas para a produção de uma dose de baculovírus formulado (dose para um hectare). A produção total de poliedros, nas diferentes temperaturas de inoculação, foi semelhante, mas a incubação a 25 °C produziu uma maior quantidade de vírus (24,56 x 109 PIB a 25 °C e 20,81 x 109 PIB a 28 °C). No segundo estudo as concentrações letais de SfMNPV foram determinadas para lagartas de seis, sete e oito dias e a melhor idade para se realizar a inoculação de SfMNPV visando a maximização de sua produção. As concentrações letais médias (CL50) variaram de 1,89 x 105 a 5,01 x 106 PIB/mL. A CL50 do isolado 6 (I6) foi maior que a do isolado 19 (I19) para lagartas de seis dias de idade, semelhante para lagartas de sete dias e menor para aquelas de oito dias. A produção total de poliedros foi maior com lagartas inoculadas aos oito dias de idade com o I6. As maiores produções virais foram obtidas nas concentrações acima de 1,00 x 107 PIB/mL. A produção de PIB/lagarta foi maior com o I6 em lagartas de oito dias, o que reduz o número de lagartas para a produção de uma dose de baculovírus formulado (dose para um hectare). O terceiro estudo objetivou verificar o parâmetro de produção mais correlacionado com a produção viral, a massa, ou o número de lagartas mortas por SfMNPV e determinar os parâmetros peso equivalente por hectare (massa estimada de lagartas necessárias para obtenção de uma dose de baculovírus formulado, tendo como base a aplicação de 2,00 x 1011 PIB/ha) e peso por lagarta morta devido à infecção pelo I6 de SfMNPV. Todas as correlações foram positivas e altamente significativas para o peso total (r= 0,958, n= 60 e P< 0,0001), mesmo quando consideradas dentro de cada idade ou concentração, separadamente. A correlação para o número de lagartas foi, também, significativa considerando-se o total de tratamentos (r= 0,723, n= 60 e P< 0,0001) e por idade. Por concentração, foi significativa apenas nas concentrações de 1,00 x 106 PIB/mL (r= 0,643, n= 12, P< 0,0242) e 1,00 x 108 PIB/mL (r= 0,657, n= 12, P< 0,0202), porém, as correlações foram menos fortes. Lagartas de S. frugiperda, mortas por SfMNPV, inoculadas aos oito dias de idade apresentaram a maior massa média. O peso equivalente de SfMNPV a ser utilizado por hectare não diferiu entre as concentrações de suspensões virais. A produção viral de baculovírus, para o controle de S. frugiperda, deve ser feita com o isolado I6 em lagartas de oito dias na temperatura de 25 oC. O peso de lagartas mortas por baculovírus pode ser considerado um parâmetro mais confiável que o número de lagartas para a produção de um produto comercial a base de SfMNPV, devido a correlação mais forte e significativa com a produção total de poliedros.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em FitotecniaUFVBRPlantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita deBaculovírusSpodoptera frugiperdaIdade larvalControle biológicoBaculovirusSpodoptera frugiperdaLarval ageBiological controlCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAMultiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)Multiplication of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf984419https://locus.ufv.br//bitstream/123456789/4617/1/texto%20completo.pdf33605a6daae90efa42a31afbfa576ba6MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain144231https://locus.ufv.br//bitstream/123456789/4617/2/texto%20completo.pdf.txt18fd225ac5cf6fd3efd2b0ca87a86242MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3401https://locus.ufv.br//bitstream/123456789/4617/3/texto%20completo.pdf.jpg1f8d6cb90963d9e6b7758e896aa9721eMD53123456789/46172016-04-10 23:11:24.404oai:locus.ufv.br:123456789/4617Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:11:24LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
dc.title.alternative.eng.fl_str_mv Multiplication of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae)
title Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
spellingShingle Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
Paiva, Carlos Eduardo Costa
Baculovírus
Spodoptera frugiperda
Idade larval
Controle biológico
Baculovirus
Spodoptera frugiperda
Larval age
Biological control
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
title_short Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
title_full Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
title_fullStr Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
title_full_unstemmed Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
title_sort Multiplicação de Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) em lagartas de Spodoptera frugiperda (Lepidoptera: Noctuidae)
author Paiva, Carlos Eduardo Costa
author_facet Paiva, Carlos Eduardo Costa
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/7544068911776191
dc.contributor.author.fl_str_mv Paiva, Carlos Eduardo Costa
dc.contributor.advisor-co1.fl_str_mv Valicente, Fernando Hercos
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781755J8
dc.contributor.advisor-co2.fl_str_mv Serrão, José Eduardo
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6
dc.contributor.advisor1.fl_str_mv Zanuncio, José Cola
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787556T2
dc.contributor.referee1.fl_str_mv Sediyama, Carlos Sigueyuki
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3647241087918239
dc.contributor.referee2.fl_str_mv Oliveira, Eugenio Eduardo de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0098564066936024
contributor_str_mv Valicente, Fernando Hercos
Serrão, José Eduardo
Zanuncio, José Cola
Sediyama, Carlos Sigueyuki
Oliveira, Eugenio Eduardo de
dc.subject.por.fl_str_mv Baculovírus
Spodoptera frugiperda
Idade larval
Controle biológico
topic Baculovírus
Spodoptera frugiperda
Idade larval
Controle biológico
Baculovirus
Spodoptera frugiperda
Larval age
Biological control
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
dc.subject.eng.fl_str_mv Baculovirus
Spodoptera frugiperda
Larval age
Biological control
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
description The use of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) based biopesticide has the potential to control Spodoptera frugiperda (Lepidoptera: Noctuidae), but large scale production depends on maximizing virus production from infected larvae. The suitable temperature, time of exposure to the pathogen, the age at which the larvae are inoculated with the virus, the viral solution concentration and isolate used are important for viral replication. The experiments were performed in the Biological Control Laboratory of Insects at Embrapa Maize and Sorghum Research Center (CNPMS), located in Sete Lagoas, Minas Gerais, Brazil. The first study aimed to verify the effect of inoculation and incubation (multiplication) temperatures in viral production and the inoculation period. Cannibalism among the larvae of S. frugiperda was similar (around 4%) in different periods and temperatures baculovirus inoculation. The inoculation period did influence mortality and viral production. The higher mortalities of larvae were achieved at 28 °C, however, the production of polyhedral inclusion bodies (PIB) per larvae were higher at 25 °C, reducing the number of larvae needed for the production of a dose of baculovirus expressed larval equivalent (LE/ha). The total production of polyhedral inoculation at the different temperatures was similar, but the incubation at 25 °C produced a larger amount of virus (24.56 x 109 PIB at 25 °C and 20.81 x 109 PIB at 28 °C). In the second study, the lethal concentrations were determined for larvae SfMNPV six, seven and eight days and the best age to perform the inoculation of SfMNPV order to maximize viral production. The lethal concentration (LC50) ranged from 1.89 x 105 to 5.01 x 106 PIB/mL. The LC50 I6 was higher than the I19 to six days old larvae of similar for seven days and for those less than eight days. Total polyhedra production was higher with eight days old larvae for the isolate I6. The highest yields were obtained in viral concentrations above 1.00 x 107 PIB/mL. The production of PIB/larvae increased when I6 was used in eight days old larvae, which reduces the number of larvae needed to achieve dose of baculovirus (LE/ha). The third study aimed to verify the production parameter which is more correlated with viral production, mass, or the number of dead larvae by SfMNPV and also determine the parameters weight equivalent per hectare (estimated larval weight necessary to achieve a dose of baculovirus, based on the application of 2.00 x 1011 PIB/ha) and larvae dead weight due to SfMNPV infection caused by I6. All correlations were positive and significant for the total weight (r= 0.958, n= 60, P< 0.0001) even when considered in each age or concentration separately. For the number of tracks the correlation was also significant when considering the amount of treatment (r= 0.723, n= 60, P< 0.0001) within each age group and in each concentration was significant only at concentrations of 1.00 x 106 PIB/mL (r= 0.643, n= 12, P< 0.0242) and 1.00 x 108 (r= 0.657, n= 12, P< 0.0202), but the correlations are less strong. Larvae of S. frugiperda, killed by SfMNPV inoculated at eight days old had the highest average mass. The equivalent weight per hectare did differ among different viral concentrations of solutions used. The maximization of viral production baculovirus for control of S. frugiperda should be made with the isolated I6 in larvae eight days old maintained at a temperature of 25 °C and the weight of dead larvae by baculoviruses can be considered a more reliable parameter than the number of larvae to produce a commercial product based SfMNPV in view that shows strong and significant correlation with the total polyhedra production.
publishDate 2013
dc.date.issued.fl_str_mv 2013-07-26
dc.date.available.fl_str_mv 2014-01-27
2015-03-26T13:40:00Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:40:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PAIVA, Carlos Eduardo Costa. Multiplication of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae). 2013. 76 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4617
identifier_str_mv PAIVA, Carlos Eduardo Costa. Multiplication of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae). 2013. 76 f. Dissertação (Mestrado em Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de) - Universidade Federal de Viçosa, Viçosa, 2013.
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dc.publisher.program.fl_str_mv Mestrado em Fitotecnia
dc.publisher.initials.fl_str_mv UFV
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Plantas daninhas, Alelopatia, Herbicidas e Resíduos; Fisiologia de culturas; Manejo pós-colheita de
publisher.none.fl_str_mv Universidade Federal de Viçosa
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