Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites

Detalhes bibliográficos
Autor(a) principal: Marques-Silva, Solange
Data de Publicação: 2004
Outros Autores: Serrão, José Eduardo, Mezêncio, José Mario S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.acthis.2004.10.003
http://www.locus.ufv.br/handle/123456789/19086
Resumo: The origin of the peritrophic membrane (PM) in bees is still a matter of debate. It is either of type I (synthesized by the entire midgut epithelium) or of type II (released from the anterior midgut end). The present study identified secretory sites of peritrophin-55 kDa, a PM protein in larvae of Melipona quadrifasciata anthidioides. Peritrophin-55 was isolated from PMs and was used for the production of a polyclonal antibody. Our study demonstrates the presence of peritrophin-55 in vesicles and on microvilli of digestive cells and in the PM. It suggests that the PM is of type-I, being specific for the larval phase of this stingless bee.
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spelling Marques-Silva, SolangeSerrão, José EduardoMezêncio, José Mario S.2018-04-24T16:58:01Z2018-04-24T16:58:01Z2004-10-240065-1281https://doi.org/10.1016/j.acthis.2004.10.003http://www.locus.ufv.br/handle/123456789/19086The origin of the peritrophic membrane (PM) in bees is still a matter of debate. It is either of type I (synthesized by the entire midgut epithelium) or of type II (released from the anterior midgut end). The present study identified secretory sites of peritrophin-55 kDa, a PM protein in larvae of Melipona quadrifasciata anthidioides. Peritrophin-55 was isolated from PMs and was used for the production of a polyclonal antibody. Our study demonstrates the presence of peritrophin-55 in vesicles and on microvilli of digestive cells and in the PM. It suggests that the PM is of type-I, being specific for the larval phase of this stingless bee.engActa HistochemicaVolume 107, Issue 1, Pages 23-30, May 2005Elsevier GmbHinfo:eu-repo/semantics/openAccessDevelopmentHymenopteraInsectMidgutPeritrophinPeritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf560964https://locus.ufv.br//bitstream/123456789/19086/1/artigo.pdf5c2883e544109a6f0172cf8274dddb31MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19086/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5260https://locus.ufv.br//bitstream/123456789/19086/3/artigo.pdf.jpgf93537f32b4837a035b93dcf82b7bd2fMD53123456789/190862018-04-24 23:00:46.273oai:locus.ufv.br:123456789/19086Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-25T02:00:46LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
title Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
spellingShingle Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
Marques-Silva, Solange
Development
Hymenoptera
Insect
Midgut
Peritrophin
title_short Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
title_full Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
title_fullStr Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
title_full_unstemmed Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
title_sort Peritrophic membrane protein in the larval stingless bee Melipona quadrifasciata anthidioides: immunolocalization of secretory sites
author Marques-Silva, Solange
author_facet Marques-Silva, Solange
Serrão, José Eduardo
Mezêncio, José Mario S.
author_role author
author2 Serrão, José Eduardo
Mezêncio, José Mario S.
author2_role author
author
dc.contributor.author.fl_str_mv Marques-Silva, Solange
Serrão, José Eduardo
Mezêncio, José Mario S.
dc.subject.pt-BR.fl_str_mv Development
Hymenoptera
Insect
Midgut
Peritrophin
topic Development
Hymenoptera
Insect
Midgut
Peritrophin
description The origin of the peritrophic membrane (PM) in bees is still a matter of debate. It is either of type I (synthesized by the entire midgut epithelium) or of type II (released from the anterior midgut end). The present study identified secretory sites of peritrophin-55 kDa, a PM protein in larvae of Melipona quadrifasciata anthidioides. Peritrophin-55 was isolated from PMs and was used for the production of a polyclonal antibody. Our study demonstrates the presence of peritrophin-55 in vesicles and on microvilli of digestive cells and in the PM. It suggests that the PM is of type-I, being specific for the larval phase of this stingless bee.
publishDate 2004
dc.date.issued.fl_str_mv 2004-10-24
dc.date.accessioned.fl_str_mv 2018-04-24T16:58:01Z
dc.date.available.fl_str_mv 2018-04-24T16:58:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.acthis.2004.10.003
http://www.locus.ufv.br/handle/123456789/19086
dc.identifier.issn.none.fl_str_mv 0065-1281
identifier_str_mv 0065-1281
url https://doi.org/10.1016/j.acthis.2004.10.003
http://www.locus.ufv.br/handle/123456789/19086
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 107, Issue 1, Pages 23-30, May 2005
dc.rights.driver.fl_str_mv Elsevier GmbH
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Elsevier GmbH
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Acta Histochemica
publisher.none.fl_str_mv Acta Histochemica
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instname:Universidade Federal de Viçosa (UFV)
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