ANÁLISES CITOGENÉTICAS EM Melipona paraensis (HYMENOPTERA: APIDAE)

• Autor(a) principal: Cassinela, Edson Kuatelela
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2368
• topic: Cromossomos metafasicos; Heterocromatina; Melipona; Metaphase chromosomes; Heterochromatin; Melipona; CNPQ::CIENCIAS BIOLOGICAS::BIOFISICA::BIOFISICA CELULAR

Cytogenetic techniques are very useful tools in studies of characterization and differentiation of species. Melipona paraensis samples were collected at Altamira in Para State and used to describe the karyotype of the species, determine the heterochromatin content, its location and base composition of chromatin, using conventional staining techniques, C-band and fluorochromes CMA3/DA / DAPI. This is the first study of cytogenetics with the stingless bee M. paraensis . Conventional staining revealed that this species has a chromosome number of 2n=18. The technique of Band C showed high content of heterochromatin that is distributed throughout the chromosome. The high content of heterochromatin positioning M. paraensis in Group II includes species of Melipona with high heterochromatin content. The DAPI fluorochrome strongly marked the region of heterochromatin indicating that these regions should be rich in AT base pairs. The CMA3 fluorochrome marked the ends of chromosomes that correspond to euchromatin. This region most heavily labeled with fluorochrome CMA3 may indicate that this is the nucleolus organizer regions. FISH showed clearer and brighter markings that were observed in specific regions of chromosomes. These markings may be indicating the position of centromere on chromosomes evaluated, this result, is the first reported in Melipona of high heterochromatin content.