Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/5355 |
Resumo: | Viruses are organisms with small genomes of simple organization, which coding about 3 to encode 10 viral proteins. The success of the infection depends on the manipulation of the cell by the virus, by means of complex interactions occurring between viral factors and host factors. The induced changes by virus infection include cell morphology changes, cell cycle changes and alterations in gene expression, among others. Understanding the processes that favor viral infection necessarily involves the study of virus-host interactions. In order to better understand the processes related to infection by tomato potyvirus Pepper yellow mosaic virus (PepYMV) a subtractive library was built 72 hours after infection. Several genes were identified as induced or repressed by viral infection. Among the induced genes, is the gene encoding the Translationally controlled tumor protein (TCTP). TCTP protein is highly conserved in all eukaryotes. Its functions are related to growth control and cell cycle, anti-apoptotic activity, and response to different biotic and abiotic stresses. The involvement of this protein in infection PepYMV has not been established, but studies in a strain of transgenic tomato plants silenced for TCTP showed that the silenced plants have a lower accumulation of PepYMV, indicating that TCTP promotes viral infection. In this study, we sought to advance the understanding of mechanisms involving TCTP in the process of infection by PepYMV. N. benthamiana plants silenced by VIGS TCTP were used to study the effect of silencing in viral infection, and the silenced plants accumulate fewer viruses in early stages of virus infection. Individual expression of viral proteins in N. benthamiana identified P3 and CP as capable of inducing TCTP expression at similar levels to those induced during PepYMV infection, and expression of NIb reduced expression of TCTP. The verification of direct interactions occurrence between viral proteins and TCTP by double-hybrid assay showed that TCTP not interact separately with any of the proteins of viral origin. Purification of proteins of health and infected N. benthamiana plants by affinity with TCTP identified several proteins that putativaly interacts with TCTP. As in two hybrid assay, interactions involving PepYMV proteins were not detected. These results sugests that TCTP actuation must involve the formation of protein complexes involving viral and plant proteins or contribute indirectly to PepYMV infection, without involving direct interactions between TCTP and viral proteins. |
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Bruckner, Fernanda Prietohttp://lattes.cnpq.br/7962672282373013Queiroz, Marisa Vieira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U52015-03-26T13:51:57Z2013-04-112015-03-26T13:51:57Z2012-07-19BRUCKNER, Fernanda Prieto. Aspects of TCTP protein interaction with the potyvirus PepYMV during infection of tomato and N. benthamiana. 2012. 81 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/5355Viruses are organisms with small genomes of simple organization, which coding about 3 to encode 10 viral proteins. The success of the infection depends on the manipulation of the cell by the virus, by means of complex interactions occurring between viral factors and host factors. The induced changes by virus infection include cell morphology changes, cell cycle changes and alterations in gene expression, among others. Understanding the processes that favor viral infection necessarily involves the study of virus-host interactions. In order to better understand the processes related to infection by tomato potyvirus Pepper yellow mosaic virus (PepYMV) a subtractive library was built 72 hours after infection. Several genes were identified as induced or repressed by viral infection. Among the induced genes, is the gene encoding the Translationally controlled tumor protein (TCTP). TCTP protein is highly conserved in all eukaryotes. Its functions are related to growth control and cell cycle, anti-apoptotic activity, and response to different biotic and abiotic stresses. The involvement of this protein in infection PepYMV has not been established, but studies in a strain of transgenic tomato plants silenced for TCTP showed that the silenced plants have a lower accumulation of PepYMV, indicating that TCTP promotes viral infection. In this study, we sought to advance the understanding of mechanisms involving TCTP in the process of infection by PepYMV. N. benthamiana plants silenced by VIGS TCTP were used to study the effect of silencing in viral infection, and the silenced plants accumulate fewer viruses in early stages of virus infection. Individual expression of viral proteins in N. benthamiana identified P3 and CP as capable of inducing TCTP expression at similar levels to those induced during PepYMV infection, and expression of NIb reduced expression of TCTP. The verification of direct interactions occurrence between viral proteins and TCTP by double-hybrid assay showed that TCTP not interact separately with any of the proteins of viral origin. Purification of proteins of health and infected N. benthamiana plants by affinity with TCTP identified several proteins that putativaly interacts with TCTP. As in two hybrid assay, interactions involving PepYMV proteins were not detected. These results sugests that TCTP actuation must involve the formation of protein complexes involving viral and plant proteins or contribute indirectly to PepYMV infection, without involving direct interactions between TCTP and viral proteins.Os vírus são organismos com genomas pequenos, de organização simples, que codificam em média 3 a 10 proteínas. O sucesso da infecção depende da manipulação da célula pelo vírus, por meio de interações complexas que ocorrem entre fatores virais e fatores do hospedeiro. As modificações induzidas na célula incluem alterações morfológicas, alteração do ciclo celular e na expressão gênica, entre outras. A compreensão dos processos que favorecem a infecção viral passa necessariamente pelo estudo de interações vírus-hospedeiro. No intuito de compreender melhor os processos relacionados à infecção de tomateiros pelo potyvírus Pepper yellow mosaic virus (PepYMV) uma biblioteca subtrativa foi construída 72 horas após a infecção. Diversos genes cuja expressão foi alterada pela infecção foram identificados. Dentre os genes induzidos, se encontra o gene que codifica a Translationally controlled tumor protein (TCTP). A proteína TCTP é altamente conservada em todos os eucariotos. Suas funções estão relacionadas a controle do crescimento e ciclo celular, atividade anti-apoptótica, e resposta a diferentes tipos de estresses abióticos e bióticos. O envolvimento desta proteína na infecção pelo PepYMV ainda não foi estabelecido, porém estudos em uma linhagem de tomateiro transgênica silenciadas para a TCTP, mostraram que as plantas silenciadas apresentam um menor acúmulo de PepYMV, indicando que a TCTP favorece a infecção por este vírus. Neste trabalho, buscou-se avançar na compreensão dos mecanismos que envolvem a TCTP no processo de infecção pelo PepYMV. Plantas de Nicotiana benthamiana silenciadas para TCTP por VIGS (Virus Induced Gene Silence) foram utilizadas para estudar o efeito do silenciamento na infecção viral, sendo que as plantas silenciadas acumularam menos vírus no início da infecção. A expressão individual das proteínas de origem viral em N. benthamiana identificou a P3 e a CP como capazes de induzir a expressão de TCTP em níveis semelhantes aos observados durante a infecção pelo PepYMV, sendo que a expressão da proteína NIb reduziu a expressão de TCTP. A verificação da ocorrência de interações diretas entre a TCTP e as proteínas virais, por ensaio de duplo híbrido, mostrou que a TCTP não interage separadamente com as proteínas de origem viral. A purificação de proteínas de plantas de N. benthamiana, sadias e infectadas, por afinidade com a TCTP identificou diversas proteínas que possivelmente 7 interagem com a TCTP. Assim como no ensaio de duplo híbrido, a interação com proteínas virais não foi detectada. Estes resultados sugerem que o papel da TCTP deve envolver a formação de complexos proteicos entre proteínas virais e da planta, ou favorecer a infecção de forma indireta.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interessePotyvirusTranslationally controlled tumor protein (TCTP)Pepper yellow mosaic virusInteração vírus-plantaPotyvirusTranslationally controlled tumor protein (TCTP)Pepper yellow mosaic virusVirus-plant interactionCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLAAspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamianaAspects of TCTP protein interaction with the potyvirus PepYMV during infection of tomato and N. benthamianainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo .pdfapplication/pdf1092167https://locus.ufv.br//bitstream/123456789/5355/1/texto%20completo%20.pdf8586b9a4bcc728e0319bbbdb012ca136MD51TEXTtexto completo .pdf.txttexto completo .pdf.txtExtracted texttext/plain187701https://locus.ufv.br//bitstream/123456789/5355/2/texto%20completo%20.pdf.txt35544eb6a03e459272d768c600b66579MD52THUMBNAILtexto completo .pdf.jpgtexto completo .pdf.jpgIM Thumbnailimage/jpeg3657https://locus.ufv.br//bitstream/123456789/5355/3/texto%20completo%20.pdf.jpg772392c7ea19bdb87334ca6397ef984cMD53123456789/53552016-04-10 23:18:25.026oai:locus.ufv.br:123456789/5355Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:18:25LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
dc.title.alternative.eng.fl_str_mv |
Aspects of TCTP protein interaction with the potyvirus PepYMV during infection of tomato and N. benthamiana |
title |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
spellingShingle |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana Bruckner, Fernanda Prieto Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Interação vírus-planta Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Virus-plant interaction CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA |
title_short |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
title_full |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
title_fullStr |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
title_full_unstemmed |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
title_sort |
Aspectos da interação entre a proteína TCTP e o potyvírus PepYMV na infecção de tomateiro e Nicotiana benthamiana |
author |
Bruckner, Fernanda Prieto |
author_facet |
Bruckner, Fernanda Prieto |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7962672282373013 |
dc.contributor.author.fl_str_mv |
Bruckner, Fernanda Prieto |
dc.contributor.advisor-co1.fl_str_mv |
Queiroz, Marisa Vieira de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5 |
dc.contributor.advisor1.fl_str_mv |
Zerbini, Poliane Alfenas |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8632328159533071 |
dc.contributor.referee1.fl_str_mv |
Zerbini Júnior, Francisco Murilo |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5 |
contributor_str_mv |
Queiroz, Marisa Vieira de Zerbini, Poliane Alfenas Zerbini Júnior, Francisco Murilo |
dc.subject.por.fl_str_mv |
Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Interação vírus-planta |
topic |
Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Interação vírus-planta Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Virus-plant interaction CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA |
dc.subject.eng.fl_str_mv |
Potyvirus Translationally controlled tumor protein (TCTP) Pepper yellow mosaic virus Virus-plant interaction |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA |
description |
Viruses are organisms with small genomes of simple organization, which coding about 3 to encode 10 viral proteins. The success of the infection depends on the manipulation of the cell by the virus, by means of complex interactions occurring between viral factors and host factors. The induced changes by virus infection include cell morphology changes, cell cycle changes and alterations in gene expression, among others. Understanding the processes that favor viral infection necessarily involves the study of virus-host interactions. In order to better understand the processes related to infection by tomato potyvirus Pepper yellow mosaic virus (PepYMV) a subtractive library was built 72 hours after infection. Several genes were identified as induced or repressed by viral infection. Among the induced genes, is the gene encoding the Translationally controlled tumor protein (TCTP). TCTP protein is highly conserved in all eukaryotes. Its functions are related to growth control and cell cycle, anti-apoptotic activity, and response to different biotic and abiotic stresses. The involvement of this protein in infection PepYMV has not been established, but studies in a strain of transgenic tomato plants silenced for TCTP showed that the silenced plants have a lower accumulation of PepYMV, indicating that TCTP promotes viral infection. In this study, we sought to advance the understanding of mechanisms involving TCTP in the process of infection by PepYMV. N. benthamiana plants silenced by VIGS TCTP were used to study the effect of silencing in viral infection, and the silenced plants accumulate fewer viruses in early stages of virus infection. Individual expression of viral proteins in N. benthamiana identified P3 and CP as capable of inducing TCTP expression at similar levels to those induced during PepYMV infection, and expression of NIb reduced expression of TCTP. The verification of direct interactions occurrence between viral proteins and TCTP by double-hybrid assay showed that TCTP not interact separately with any of the proteins of viral origin. Purification of proteins of health and infected N. benthamiana plants by affinity with TCTP identified several proteins that putativaly interacts with TCTP. As in two hybrid assay, interactions involving PepYMV proteins were not detected. These results sugests that TCTP actuation must involve the formation of protein complexes involving viral and plant proteins or contribute indirectly to PepYMV infection, without involving direct interactions between TCTP and viral proteins. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-19 |
dc.date.available.fl_str_mv |
2013-04-11 2015-03-26T13:51:57Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:51:57Z |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
BRUCKNER, Fernanda Prieto. Aspects of TCTP protein interaction with the potyvirus PepYMV during infection of tomato and N. benthamiana. 2012. 81 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/5355 |
identifier_str_mv |
BRUCKNER, Fernanda Prieto. Aspects of TCTP protein interaction with the potyvirus PepYMV during infection of tomato and N. benthamiana. 2012. 81 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2012. |
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Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse |
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Universidade Federal de Viçosa |
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