Micropropagação em biorreatores de imersão temporária e enraizamento de miniestacas e microestacas de clones híbridos de Eucalyptus globulus

• Autor(a) principal: Correia, Anne Caroline Guieiro
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/3088
• topic: Propagação in vitro; Propagação vegetativa; Silvicultura clonal; Propagation in vitro; Vegetative propagation; Clonal forestry; CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA

This study aimed to evaluate the micropropagation through temporary immersion bioreactors RITA ® multiplication and elongation in vitro, as well as mini-cuttings and microcutting the rooting of clones Eucalyptus urophylla x E. globulus and Eucalyptus grandis x E. globulus. In the multiplication phase and stretching evaluated the cultivation liquid system (bioreactor RITA ® ) and semisolid (plastic bottles and test tubes), and the ratios between the different sources of nitrogen (nitrate / ammonium) on growth and productivity explants. The influence of propagation techniques, ( micro and mini-cuttings), different types of cuttings and substrates (with and without apical and middle leaf reduction) in the rooting of clones hybrid Eucalyptus globulus clones. It was found that the bioreactor of temporary immersion RITA ® and plastic pots showed the best results in the growth of explants. There was no difference between the reasons for nitrate nitrogen (NO3-) and ammonium (NH4 +) (1:1, 2:1 and 3:1) used in the multiplication phase and elongation, except for clone C16 in the phase of multiplication obtained greater growth and productivity in the ratio 2:1 (NO3-): (NH4 +). Overall cultures had a high degree of hyper-hydro, which is a disorder limiting factor in this study conditions for micropropagation in bioreactor of temporary immersion RITA ®. There was no significant superiority of microcuttings regarding mini-cuttings and was no effect of different substrates for only a few features. In overall, the of apical cuttings (without reduction and reduced leaf) was superiority in relation the intermediate.