In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s11627-014-9651-z http://www.locus.ufv.br/handle/123456789/22276 |
Resumo: | This study aimed to establish a protocol for in vitro propagation of two accessions (Ac) of Pfaffia glomerata (Ac 4 and Ac 13) and to evaluate the effect of different carbon sources on the production of 20-hydroxyecdysone (20E) in leaves and roots. For the assessment of axillary shoot proliferation in vitro, nodal segments were inoculated onto Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzyladenine and 2.68 μM α-naphthaleneacetic acid and carbon sources (glucose or sucrose) at varying concentrations (0.1, 0.2, or 0.3 M). To assess the in vitro production of 20E, nodal segments were inoculated into Magenta® containers containing MS medium with different carbon sources (glucose, sucrose, or glucose + sucrose at 0.1 or 0.2 M) and placed in plastic bags with bacterial filters. Both experiments were composed of five repetitions for each treatment and analyzed after 30 d of culture. Multiple shoot formations were genotype-dependent when segments were cultivated on a medium supplemented with glucose or sucrose at 0.1 M, yielding 35 and 43 shoots per explant for Ac 4 and 4.4 and 2.8 shoots per explant for Ac 13, respectively. For the 20E content, significant effects were also observed among accessions and carbon sources. Ac 13 had the highest average 20E levels for both roots and leaves. Under the experimental conditions, Ac 4 had more favorable characteristics for large-scale multiplication than Ac 13, and glucose at 0.2 M was the best carbon source for the cultivation of Pfaffia, both for producing multiple shoots and for in vitro 20E production. This is the first report using a combination of auxin and cytokinin to enable effective Pfaffia in vitro axillary shoot proliferation from nodal explants. |
id |
UFV_b2954b8ec455499c552bed4ccc3f57d8 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/22276 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Vasconcelos, Jaqueline MartinsSaldanha, Cleber WittDias, Leonardo Lucas CarnevalliMaldaner, JoseilaRêgo, Mailson MonteiroSilva, Luzimar CamposOtoni, Wagner Campos2018-10-16T11:56:33Z2018-10-16T11:56:33Z2014-11-0614752689http://dx.doi.org/10.1007/s11627-014-9651-zhttp://www.locus.ufv.br/handle/123456789/22276This study aimed to establish a protocol for in vitro propagation of two accessions (Ac) of Pfaffia glomerata (Ac 4 and Ac 13) and to evaluate the effect of different carbon sources on the production of 20-hydroxyecdysone (20E) in leaves and roots. For the assessment of axillary shoot proliferation in vitro, nodal segments were inoculated onto Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzyladenine and 2.68 μM α-naphthaleneacetic acid and carbon sources (glucose or sucrose) at varying concentrations (0.1, 0.2, or 0.3 M). To assess the in vitro production of 20E, nodal segments were inoculated into Magenta® containers containing MS medium with different carbon sources (glucose, sucrose, or glucose + sucrose at 0.1 or 0.2 M) and placed in plastic bags with bacterial filters. Both experiments were composed of five repetitions for each treatment and analyzed after 30 d of culture. Multiple shoot formations were genotype-dependent when segments were cultivated on a medium supplemented with glucose or sucrose at 0.1 M, yielding 35 and 43 shoots per explant for Ac 4 and 4.4 and 2.8 shoots per explant for Ac 13, respectively. For the 20E content, significant effects were also observed among accessions and carbon sources. Ac 13 had the highest average 20E levels for both roots and leaves. Under the experimental conditions, Ac 4 had more favorable characteristics for large-scale multiplication than Ac 13, and glucose at 0.2 M was the best carbon source for the cultivation of Pfaffia, both for producing multiple shoots and for in vitro 20E production. This is the first report using a combination of auxin and cytokinin to enable effective Pfaffia in vitro axillary shoot proliferation from nodal explants.engIn Vitro Cellular & Developmental Biology - Plantv. 50, n. 6, p. 746– 751, dez. 2014The Society for In Vitro Biologyinfo:eu-repo/semantics/openAccessAxillary proliferationCarbon sourceGenotypeMicropropagationPhytoecdysteroidOrganogenesisIn vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sourcesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf417392https://locus.ufv.br//bitstream/123456789/22276/1/artigo.pdf9d1a7e2176fba83bf230f653e05028fdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22276/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/222762018-10-16 09:05:37.144oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-10-16T12:05:37LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
title |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
spellingShingle |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources Vasconcelos, Jaqueline Martins Axillary proliferation Carbon source Genotype Micropropagation Phytoecdysteroid Organogenesis |
title_short |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
title_full |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
title_fullStr |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
title_full_unstemmed |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
title_sort |
In vitro propagation of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources |
author |
Vasconcelos, Jaqueline Martins |
author_facet |
Vasconcelos, Jaqueline Martins Saldanha, Cleber Witt Dias, Leonardo Lucas Carnevalli Maldaner, Joseila Rêgo, Mailson Monteiro Silva, Luzimar Campos Otoni, Wagner Campos |
author_role |
author |
author2 |
Saldanha, Cleber Witt Dias, Leonardo Lucas Carnevalli Maldaner, Joseila Rêgo, Mailson Monteiro Silva, Luzimar Campos Otoni, Wagner Campos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vasconcelos, Jaqueline Martins Saldanha, Cleber Witt Dias, Leonardo Lucas Carnevalli Maldaner, Joseila Rêgo, Mailson Monteiro Silva, Luzimar Campos Otoni, Wagner Campos |
dc.subject.pt-BR.fl_str_mv |
Axillary proliferation Carbon source Genotype Micropropagation Phytoecdysteroid Organogenesis |
topic |
Axillary proliferation Carbon source Genotype Micropropagation Phytoecdysteroid Organogenesis |
description |
This study aimed to establish a protocol for in vitro propagation of two accessions (Ac) of Pfaffia glomerata (Ac 4 and Ac 13) and to evaluate the effect of different carbon sources on the production of 20-hydroxyecdysone (20E) in leaves and roots. For the assessment of axillary shoot proliferation in vitro, nodal segments were inoculated onto Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzyladenine and 2.68 μM α-naphthaleneacetic acid and carbon sources (glucose or sucrose) at varying concentrations (0.1, 0.2, or 0.3 M). To assess the in vitro production of 20E, nodal segments were inoculated into Magenta® containers containing MS medium with different carbon sources (glucose, sucrose, or glucose + sucrose at 0.1 or 0.2 M) and placed in plastic bags with bacterial filters. Both experiments were composed of five repetitions for each treatment and analyzed after 30 d of culture. Multiple shoot formations were genotype-dependent when segments were cultivated on a medium supplemented with glucose or sucrose at 0.1 M, yielding 35 and 43 shoots per explant for Ac 4 and 4.4 and 2.8 shoots per explant for Ac 13, respectively. For the 20E content, significant effects were also observed among accessions and carbon sources. Ac 13 had the highest average 20E levels for both roots and leaves. Under the experimental conditions, Ac 4 had more favorable characteristics for large-scale multiplication than Ac 13, and glucose at 0.2 M was the best carbon source for the cultivation of Pfaffia, both for producing multiple shoots and for in vitro 20E production. This is the first report using a combination of auxin and cytokinin to enable effective Pfaffia in vitro axillary shoot proliferation from nodal explants. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-06 |
dc.date.accessioned.fl_str_mv |
2018-10-16T11:56:33Z |
dc.date.available.fl_str_mv |
2018-10-16T11:56:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11627-014-9651-z http://www.locus.ufv.br/handle/123456789/22276 |
dc.identifier.issn.none.fl_str_mv |
14752689 |
identifier_str_mv |
14752689 |
url |
http://dx.doi.org/10.1007/s11627-014-9651-z http://www.locus.ufv.br/handle/123456789/22276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 50, n. 6, p. 746– 751, dez. 2014 |
dc.rights.driver.fl_str_mv |
The Society for In Vitro Biology info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
The Society for In Vitro Biology |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
In Vitro Cellular & Developmental Biology - Plant |
publisher.none.fl_str_mv |
In Vitro Cellular & Developmental Biology - Plant |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/22276/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/22276/2/license.txt |
bitstream.checksum.fl_str_mv |
9d1a7e2176fba83bf230f653e05028fd 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212847738322944 |