Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling

Detalhes bibliográficos
Autor(a) principal: Visser, Evan Michael
Data de Publicação: 2015
Outros Autores: Leal, Tiago Ferreira, Almeida, Maíra Nicolau de, Guimarães, Valéria Monteze
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1186/s13068-014-0185-8
http://www.locus.ufv.br/handle/123456789/12666
Resumo: Development of efficient methods for production of renewable fuels from lignocellulosic biomass is necessary to maximize yields and reduce operating costs. One of the main challenges to industrial application of the lignocellulosic conversion process is the high costs of cellulolytic enzymes. Recycling of enzymes may present a potential solution to alleviate this problem. In the present study enzymes associated with the insoluble fraction were recycled after enzymatic hydrolysis of pretreated sugarcane bagasse, utilizing different processing conditions, enzyme loadings, and solid loadings. It was found that the enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum was efficient for enzymatic hydrolysis and that a significant portion of enzyme activity could be recovered upon recycling of the insoluble fraction. Enzyme productivity values (g glucose/mg enzyme protein) over all recycle periods were 2.4 and 3.7 for application of 15 and 30 FPU/g of glucan, representing an increase in excess of ten times that obtained in a batch process with the same enzyme blend and an even greater increase compared to commercial cellulase enzymes. Contrary to what may be expected, increasing lignin concentrations throughout the recycle period did not negatively influence hydrolysis efficiency, but conversion efficiencies continuously improved. Recycling of the entire insoluble solids fraction was sufficient for recycling of adhered enzymes together with biomass, indicative of an effective method to increase enzyme productivity.
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spelling Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recyclingEnzyme recyclingCellulosic biofuelsBioethanolGlucoseLigninDevelopment of efficient methods for production of renewable fuels from lignocellulosic biomass is necessary to maximize yields and reduce operating costs. One of the main challenges to industrial application of the lignocellulosic conversion process is the high costs of cellulolytic enzymes. Recycling of enzymes may present a potential solution to alleviate this problem. In the present study enzymes associated with the insoluble fraction were recycled after enzymatic hydrolysis of pretreated sugarcane bagasse, utilizing different processing conditions, enzyme loadings, and solid loadings. It was found that the enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum was efficient for enzymatic hydrolysis and that a significant portion of enzyme activity could be recovered upon recycling of the insoluble fraction. Enzyme productivity values (g glucose/mg enzyme protein) over all recycle periods were 2.4 and 3.7 for application of 15 and 30 FPU/g of glucan, representing an increase in excess of ten times that obtained in a batch process with the same enzyme blend and an even greater increase compared to commercial cellulase enzymes. Contrary to what may be expected, increasing lignin concentrations throughout the recycle period did not negatively influence hydrolysis efficiency, but conversion efficiencies continuously improved. Recycling of the entire insoluble solids fraction was sufficient for recycling of adhered enzymes together with biomass, indicative of an effective method to increase enzyme productivity.Biotechnology for Biofuels2017-11-01T09:54:10Z2017-11-01T09:54:10Z2015-01-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf17546834https://doi.org/10.1186/s13068-014-0185-8http://www.locus.ufv.br/handle/123456789/12666eng8:5, January 2015Visser, Evan MichaelLeal, Tiago FerreiraAlmeida, Maíra Nicolau deGuimarães, Valéria Montezeinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:35:26Zoai:locus.ufv.br:123456789/12666Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:35:26LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
title Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
spellingShingle Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
Visser, Evan Michael
Enzyme recycling
Cellulosic biofuels
Bioethanol
Glucose
Lignin
title_short Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
title_full Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
title_fullStr Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
title_full_unstemmed Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
title_sort Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling
author Visser, Evan Michael
author_facet Visser, Evan Michael
Leal, Tiago Ferreira
Almeida, Maíra Nicolau de
Guimarães, Valéria Monteze
author_role author
author2 Leal, Tiago Ferreira
Almeida, Maíra Nicolau de
Guimarães, Valéria Monteze
author2_role author
author
author
dc.contributor.author.fl_str_mv Visser, Evan Michael
Leal, Tiago Ferreira
Almeida, Maíra Nicolau de
Guimarães, Valéria Monteze
dc.subject.por.fl_str_mv Enzyme recycling
Cellulosic biofuels
Bioethanol
Glucose
Lignin
topic Enzyme recycling
Cellulosic biofuels
Bioethanol
Glucose
Lignin
description Development of efficient methods for production of renewable fuels from lignocellulosic biomass is necessary to maximize yields and reduce operating costs. One of the main challenges to industrial application of the lignocellulosic conversion process is the high costs of cellulolytic enzymes. Recycling of enzymes may present a potential solution to alleviate this problem. In the present study enzymes associated with the insoluble fraction were recycled after enzymatic hydrolysis of pretreated sugarcane bagasse, utilizing different processing conditions, enzyme loadings, and solid loadings. It was found that the enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum was efficient for enzymatic hydrolysis and that a significant portion of enzyme activity could be recovered upon recycling of the insoluble fraction. Enzyme productivity values (g glucose/mg enzyme protein) over all recycle periods were 2.4 and 3.7 for application of 15 and 30 FPU/g of glucan, representing an increase in excess of ten times that obtained in a batch process with the same enzyme blend and an even greater increase compared to commercial cellulase enzymes. Contrary to what may be expected, increasing lignin concentrations throughout the recycle period did not negatively influence hydrolysis efficiency, but conversion efficiencies continuously improved. Recycling of the entire insoluble solids fraction was sufficient for recycling of adhered enzymes together with biomass, indicative of an effective method to increase enzyme productivity.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-22
2017-11-01T09:54:10Z
2017-11-01T09:54:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 17546834
https://doi.org/10.1186/s13068-014-0185-8
http://www.locus.ufv.br/handle/123456789/12666
identifier_str_mv 17546834
url https://doi.org/10.1186/s13068-014-0185-8
http://www.locus.ufv.br/handle/123456789/12666
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 8:5, January 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Biotechnology for Biofuels
publisher.none.fl_str_mv Biotechnology for Biofuels
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
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institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
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