Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI

Detalhes bibliográficos
Autor(a) principal: Brito, Rute Magalhães
Data de Publicação: 2003
Outros Autores: Caixeiro, Ana Paula de Almeida, Pompolo, Silvia das Graças, Azevedo, Giselle Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1415-47572003000100009
http://locus.ufv.br//handle/123456789/26288
Resumo: The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH.
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spelling Brito, Rute MagalhãesCaixeiro, Ana Paula de AlmeidaPompolo, Silvia das GraçasAzevedo, Giselle Garcia2019-07-22T12:58:12Z2019-07-22T12:58:12Z20031678-4685http://dx.doi.org/10.1590/S1415-47572003000100009http://locus.ufv.br//handle/123456789/26288The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH.engGenetics and Molecular Biologyv. 26, n. 1, p. 53- 57, 2003MeliponiniPartamonaKaryotypeC- bandingFluorochromesCytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPIinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf178353https://locus.ufv.br//bitstream/123456789/26288/1/artigo.pdf5a326bcc68249bb8b446164bb831958bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/26288/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/262882019-07-22 11:14:00.124oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-07-22T14:14LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
title Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
spellingShingle Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
Brito, Rute Magalhães
Meliponini
Partamona
Karyotype
C- banding
Fluorochromes
title_short Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
title_full Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
title_fullStr Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
title_full_unstemmed Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
title_sort Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
author Brito, Rute Magalhães
author_facet Brito, Rute Magalhães
Caixeiro, Ana Paula de Almeida
Pompolo, Silvia das Graças
Azevedo, Giselle Garcia
author_role author
author2 Caixeiro, Ana Paula de Almeida
Pompolo, Silvia das Graças
Azevedo, Giselle Garcia
author2_role author
author
author
dc.contributor.author.fl_str_mv Brito, Rute Magalhães
Caixeiro, Ana Paula de Almeida
Pompolo, Silvia das Graças
Azevedo, Giselle Garcia
dc.subject.pt-BR.fl_str_mv Meliponini
Partamona
Karyotype
C- banding
Fluorochromes
topic Meliponini
Partamona
Karyotype
C- banding
Fluorochromes
description The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-07-22T12:58:12Z
dc.date.available.fl_str_mv 2019-07-22T12:58:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572003000100009
http://locus.ufv.br//handle/123456789/26288
dc.identifier.issn.none.fl_str_mv 1678-4685
identifier_str_mv 1678-4685
url http://dx.doi.org/10.1590/S1415-47572003000100009
http://locus.ufv.br//handle/123456789/26288
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 26, n. 1, p. 53- 57, 2003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
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instname:Universidade Federal de Viçosa (UFV)
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reponame_str LOCUS Repositório Institucional da UFV
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