Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572003000100009 http://locus.ufv.br//handle/123456789/26288 |
Resumo: | The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH. |
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Brito, Rute MagalhãesCaixeiro, Ana Paula de AlmeidaPompolo, Silvia das GraçasAzevedo, Giselle Garcia2019-07-22T12:58:12Z2019-07-22T12:58:12Z20031678-4685http://dx.doi.org/10.1590/S1415-47572003000100009http://locus.ufv.br//handle/123456789/26288The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH.engGenetics and Molecular Biologyv. 26, n. 1, p. 53- 57, 2003MeliponiniPartamonaKaryotypeC- bandingFluorochromesCytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPIinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf178353https://locus.ufv.br//bitstream/123456789/26288/1/artigo.pdf5a326bcc68249bb8b446164bb831958bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/26288/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/262882019-07-22 11:14:00.124oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-07-22T14:14LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
title |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
spellingShingle |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI Brito, Rute Magalhães Meliponini Partamona Karyotype C- banding Fluorochromes |
title_short |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
title_full |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
title_fullStr |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
title_full_unstemmed |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
title_sort |
Cytogenetic data of Partamona peckolti ( Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/ DAPI |
author |
Brito, Rute Magalhães |
author_facet |
Brito, Rute Magalhães Caixeiro, Ana Paula de Almeida Pompolo, Silvia das Graças Azevedo, Giselle Garcia |
author_role |
author |
author2 |
Caixeiro, Ana Paula de Almeida Pompolo, Silvia das Graças Azevedo, Giselle Garcia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Brito, Rute Magalhães Caixeiro, Ana Paula de Almeida Pompolo, Silvia das Graças Azevedo, Giselle Garcia |
dc.subject.pt-BR.fl_str_mv |
Meliponini Partamona Karyotype C- banding Fluorochromes |
topic |
Meliponini Partamona Karyotype C- banding Fluorochromes |
description |
The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of P. peckolti correspond to nucleolar organizer regions, as previously confirmed for another Partamona species by FISH. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2019-07-22T12:58:12Z |
dc.date.available.fl_str_mv |
2019-07-22T12:58:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572003000100009 http://locus.ufv.br//handle/123456789/26288 |
dc.identifier.issn.none.fl_str_mv |
1678-4685 |
identifier_str_mv |
1678-4685 |
url |
http://dx.doi.org/10.1590/S1415-47572003000100009 http://locus.ufv.br//handle/123456789/26288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 26, n. 1, p. 53- 57, 2003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Genetics and Molecular Biology |
publisher.none.fl_str_mv |
Genetics and Molecular Biology |
dc.source.none.fl_str_mv |
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Universidade Federal de Viçosa (UFV) |
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UFV |
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UFV |
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LOCUS Repositório Institucional da UFV |
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LOCUS Repositório Institucional da UFV |
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https://locus.ufv.br//bitstream/123456789/26288/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/26288/2/license.txt |
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