Avaliação da atividade biológica e obtenção de lipossomos contendo extrato de flores de Senna macranthera

• Autor(a) principal: Inoue, Flavia de Andrade
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2471
• topic: Plantas medicinais; Senna macranthera; Antibacteriano; Antioxidante; Crisofanol; Medicinal plants; Senna macranthera; Antibacterial; Antioxidant; Chrysophanol; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::ETNOFARMACOLOGIA

Plants and natural products of several different origins have been utilized for different goals. Among the most utilized medical plants, just a few have efficiency proved and tested. Among the plant types used in the popular medicine and found in the Brazilian pharmacopeia, the genus Senna is of great importance, with different activities that range from laxative to antibacterial activity. Within this genus, the species Senna macranthera (sicklepod) a tree frequently found in the woods but very used as ornamental tree in urban forestry. The flowering season are from April to June, when it becomes covered with large bright-yellow flowers The present study aimed to evaluate the antimicrobial, antioxidant and antitumor activity from the crude extracts hexane, dichloromethane ethyl acetate and ethanol obtained of the flower S. macranthera, the incorporation of the active crude extract in liposomes, and the isolation and structural identification of anthraquinones. The antibacterial activity against strains isolated from animals with bovine mastitis manifestation was positive for polar crude extracts ethyl acetate and ethanol. The crude extract ethyl acetate showed the lowest minimum inhibitory concentration for S. aureus and S. agalactiae strains. The crude extracts ethyl acetate and ethanol showed significant antioxidant activity when compared to standard compound BHT a 5 % probability with antioxidant activity values above 88%. The antitumor test, in turn, did not show good activity due to the necessity of using high concentrations of the crude extract to cause damage to cytotoxic cells. Through the technique of dynamic light scattering, we verified the formation of liposomes, suggesting the incorporation of the crude extract ethyl acetate because of a small variation in diameter (452 nm without the extract and 543 nm with it) and a decrease in light intensity with extract in a value equal to 60% of the value without the extract. The phytochemical screening of the crude extracts suggested the presence of aglucones and anthraquinones glycosides, in the polar crude extracts. The isolation and identification of chrysophanol in crude extract ethyl acetate corroborated with the phytochemical screening for the presence of anthraquinones compounds.