Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes

Detalhes bibliográficos
Autor(a) principal: Marthe, Jefferson de Brito
Data de Publicação: 2009
Outros Autores: Pompolo, Silvia das Graças, Campos, Lucio Antônio de Oliveira, Salomão, Tânia Maria Fernandes, Tavares, Mara Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1415-47572010005000029
http://www.locus.ufv.br/handle/123456789/12722
Resumo: Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.
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spelling Marthe, Jefferson de BritoPompolo, Silvia das GraçasCampos, Lucio Antônio de OliveiraSalomão, Tânia Maria FernandesTavares, Mara Garcia2017-11-01T16:52:42Z2017-11-01T16:52:42Z2009-11-0314154757http://dx.doi.org/10.1590/S1415-47572010005000029http://www.locus.ufv.br/handle/123456789/12722Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.engGenetics and Molecular Biologyvol.33 n.2, p.253-255, Apr. 2010B chromosomeSCARStingless beesCytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL2009-189 comunicacao.pdf2009-189 comunicacao.pdftexto completoapplication/pdf81901https://locus.ufv.br//bitstream/123456789/12722/1/2009-189%20comunicacao.pdf0226aeaedb2dd17827ee9ac4c16c4ac5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12722/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL2009-189 comunicacao.pdf.jpg2009-189 comunicacao.pdf.jpgIM Thumbnailimage/jpeg5078https://locus.ufv.br//bitstream/123456789/12722/3/2009-189%20comunicacao.pdf.jpgadee9316d56aa438b2314f6d5cb8d46fMD53123456789/127222017-11-01 22:01:01.907oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-02T01:01:01LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
title Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
spellingShingle Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
Marthe, Jefferson de Brito
B chromosome
SCAR
Stingless bees
title_short Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
title_full Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
title_fullStr Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
title_full_unstemmed Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
title_sort Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
author Marthe, Jefferson de Brito
author_facet Marthe, Jefferson de Brito
Pompolo, Silvia das Graças
Campos, Lucio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
author_role author
author2 Pompolo, Silvia das Graças
Campos, Lucio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marthe, Jefferson de Brito
Pompolo, Silvia das Graças
Campos, Lucio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
dc.subject.pt-BR.fl_str_mv B chromosome
SCAR
Stingless bees
topic B chromosome
SCAR
Stingless bees
description Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.
publishDate 2009
dc.date.issued.fl_str_mv 2009-11-03
dc.date.accessioned.fl_str_mv 2017-11-01T16:52:42Z
dc.date.available.fl_str_mv 2017-11-01T16:52:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572010005000029
http://www.locus.ufv.br/handle/123456789/12722
dc.identifier.issn.none.fl_str_mv 14154757
identifier_str_mv 14154757
url http://dx.doi.org/10.1590/S1415-47572010005000029
http://www.locus.ufv.br/handle/123456789/12722
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv vol.33 n.2, p.253-255, Apr. 2010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
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reponame_str LOCUS Repositório Institucional da UFV
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