Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas

Detalhes bibliográficos
Autor(a) principal: Domiciano, Gisele Pereira
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4435
Resumo: The objective of this work was to evaluate the effect of silicon (Si) in some resistance components of wheat plants against brown leaf spot (BLS), caused by Bipolaris sorokiniana as well as, physiological and biochemical defence responses induced by this element. To do so, plants of two wheat cultivars, BR-18 and BRS- 208, were grown in pots containing 0 and 0.30 g Si/kg of soil and inoculated 45 days after emergency with B. sorokiniana. Incubation period (IP), mean lesion number by cm2 of leaf area (MLN), mean lesion size (MLS) and the area under the disease progress curve (AUDPC) were measured to evaluated silicon effect on resistance components. Photosynthetic rate (PR), total soluble phenolyc compounds concentration (TSPCC), lignin acid tioglycolic derivates concentration (LATDC) and enzymatic activity of quitinases (QUI), peroxidases (POX) and poliphenoloxidase (POD) were measured to evaluate if silicon could induce physiological and biochemical responses. It was verified in this study that silicon concentration was 90,5% higher in plants supplied with this element in comparison with control plants. Moreover, it was observed a higher level of silicon deposition on leafs of both cultivars supplied with this element was revealed by means of X-rays microanalysis. In these plants the IP was prolonged in near 10h in silicon supplied plants, possibly due to physical blocking effect that this element have on fungus penetration. It was also verified that in plant supplied with silicon this element reduced the MLN, the MLS and the AUDPC in 30%, 58% and 68%, respectively, in comparison with control plants. And more, silicon concentrations correlated positively with IP (r = 0.62) and negatively with AUDPC and MLS (r = -0.57 and -0.70, respectively). The resistance components of plants supplied with Si and inoculated with B. sorokiniana were different from those measured in plants inoculated e not supplied with this element. Since silicon was the only source of variation in the experiments it can be conclude that Si enhances resistance of wheat plants against spot brown. The PR was reduced in leafs and flag leafs of plants of both cultivars after 48 hours after inoculation (HAI). However, in plants supplied with silicon, the PR was kept higher during all evaluation, possibly due to low severity of BLS in these plants. Alterations in TSPCC and LATDC due to silicon effect were also observed. Even though TSPCC oscillated a bit, it was significantly lower in leafs of silicon supplied plants during the evaluation time, although it ́s unlike that it contributed for enhancing wheat resistance to BLS. However, in the presence of silicon the decrease in TSPCC was accompanied with increase of LATDC and decrease in BLS severity. Higher concentrations of LATD in plants supplied with silicon and inoculated with B. sorokiniana, may have contributed to enhancement of cultivars resistance against BLS. QUI activity was significantly enhanced in plants of both cultivars supplied with silicon after 48 HAI. POX activity was increased only in plants of cultivar BRS- 208 supplied with silicon. Since plants supplied with silicon showed low BSP severity the high activity of QUI and POX may indicate that these enzymes participates in wheat defense against BLS. PFO activity, however, did not significantly changed between plants supplied or not with silicon. The results of this study are first evidences that silicon affects BLS severity by altering wheat resistance components to BLS and by the potentialization of physiological and biochemical responses. Silicon has then, a great potential to be used as a BLS management measure in near future.
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spelling Domiciano, Gisele Pereirahttp://lattes.cnpq.br/0212901405730925Rodrigues, Fabrício de ávilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6Jesus Júnior, Waldir Cintra dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737931T8Vale, Francisco Xavier Ribeiro dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788182P7Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4Dhingra, Onkar Devhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788051H52015-03-26T13:37:54Z2015-03-062015-03-26T13:37:54Z2008-02-21DOMICIANO, Gisele Pereira. Silicon effect on brown leaf spot development: resistance components and physiological and biochemical responses. 2008. 62 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/4435The objective of this work was to evaluate the effect of silicon (Si) in some resistance components of wheat plants against brown leaf spot (BLS), caused by Bipolaris sorokiniana as well as, physiological and biochemical defence responses induced by this element. To do so, plants of two wheat cultivars, BR-18 and BRS- 208, were grown in pots containing 0 and 0.30 g Si/kg of soil and inoculated 45 days after emergency with B. sorokiniana. Incubation period (IP), mean lesion number by cm2 of leaf area (MLN), mean lesion size (MLS) and the area under the disease progress curve (AUDPC) were measured to evaluated silicon effect on resistance components. Photosynthetic rate (PR), total soluble phenolyc compounds concentration (TSPCC), lignin acid tioglycolic derivates concentration (LATDC) and enzymatic activity of quitinases (QUI), peroxidases (POX) and poliphenoloxidase (POD) were measured to evaluate if silicon could induce physiological and biochemical responses. It was verified in this study that silicon concentration was 90,5% higher in plants supplied with this element in comparison with control plants. Moreover, it was observed a higher level of silicon deposition on leafs of both cultivars supplied with this element was revealed by means of X-rays microanalysis. In these plants the IP was prolonged in near 10h in silicon supplied plants, possibly due to physical blocking effect that this element have on fungus penetration. It was also verified that in plant supplied with silicon this element reduced the MLN, the MLS and the AUDPC in 30%, 58% and 68%, respectively, in comparison with control plants. And more, silicon concentrations correlated positively with IP (r = 0.62) and negatively with AUDPC and MLS (r = -0.57 and -0.70, respectively). The resistance components of plants supplied with Si and inoculated with B. sorokiniana were different from those measured in plants inoculated e not supplied with this element. Since silicon was the only source of variation in the experiments it can be conclude that Si enhances resistance of wheat plants against spot brown. The PR was reduced in leafs and flag leafs of plants of both cultivars after 48 hours after inoculation (HAI). However, in plants supplied with silicon, the PR was kept higher during all evaluation, possibly due to low severity of BLS in these plants. Alterations in TSPCC and LATDC due to silicon effect were also observed. Even though TSPCC oscillated a bit, it was significantly lower in leafs of silicon supplied plants during the evaluation time, although it ́s unlike that it contributed for enhancing wheat resistance to BLS. However, in the presence of silicon the decrease in TSPCC was accompanied with increase of LATDC and decrease in BLS severity. Higher concentrations of LATD in plants supplied with silicon and inoculated with B. sorokiniana, may have contributed to enhancement of cultivars resistance against BLS. QUI activity was significantly enhanced in plants of both cultivars supplied with silicon after 48 HAI. POX activity was increased only in plants of cultivar BRS- 208 supplied with silicon. Since plants supplied with silicon showed low BSP severity the high activity of QUI and POX may indicate that these enzymes participates in wheat defense against BLS. PFO activity, however, did not significantly changed between plants supplied or not with silicon. The results of this study are first evidences that silicon affects BLS severity by altering wheat resistance components to BLS and by the potentialization of physiological and biochemical responses. Silicon has then, a great potential to be used as a BLS management measure in near future.Este trabalho objetivou avaliar o efeito do silício (Si) em alguns componentes de resistência de plantas de trigo à mancha marrom causada por Bipolaris sorokiniana bem como, respostas fisiológicas e bioquímicas potencializadas por esse elemento. Para isso, plantas das cultivares de trigo, BR-18 e BRS-208 foram crescidas em vasos contendo 0 e 0,30 g Si/kg de solo e inoculadas aos 45 dias após emergência com B. sorokiniana. Foram avaliados os componentes de resistência: período de incubação (PI), número médio de lesões por cm2 de área foliar (NL), tamanho médio de lesão (TML) e área abaixo da curva do progresso da mancha marrom (AACPMM). Para avaliar se o Si poderia potencializar respostas fisiológicas e bioquímicas mensurou-se a taxa fotossintética (TF), a concentração de compostos fenólicos solúveis totais (CFST), a concentração de derivados da lignina-ácido tioglicólico (LATG), e a atividade das enzimas quitinases (QUI), peroxidades (POX) e polifenoloxidases (PFO). Neste estudo verificou-se que a concentração foliar de Si foi 90,5% superior em plantas supridas com esse elemento em relação às plantas não supridas com Si. Nas folhas das plantas supridas com Si foi observado grande deposição de Si em ambas as cultivares por meio de microanálise de raios-X. Nessas plantas, o PI foi prolongado em cerca de 10 h, possivelmente pelo impedimento físico à penetração do fungo oriundo da deposição desse elemento na superfície foliar. Verificou-se também que em plantas supridas com Si este elemento reduziu o NML, o TML e a AACPMM em 38%, 58% e 62%, respectivamente, em relação a plantas não supridas com Si. Além disso, as concentrações foliares de Si correlacionaram-se positivamente com o PI (r = 0,62) e negativamente com a AACPMM e o TML (r = -0,57 e -0,70, respectivamente). Os componentes de resistência de plantas supridas com Si e inoculadas com B. sorokiniana foram diferentes daqueles mensurados em plantas inoculadas e não supridas com esse elemento. Como houve variação apenas desse elemento nos experimentos podemos concluir que o Si aumentou a resistência de plantas de trigo à mancha marrom. A TF foi reduzida nas folhas bandeira e em folhas abaixo da folha bandeira das plantas das duas cultivares de trigo a partir de 48 horas após a inoculação (HAI). Porém, em plantas supridas com Si a TF manteve-se mais elevada no decorrer das avaliações, possivelmente devido a menor severidade da mancha marrom nessas plantas. Alterações na produção de CFST e na concentração dos derivados da LATG também foram observadas. Apesar da produção de CFST ter oscilado, esta foi significativamente menor em folhas de plantas supridas com Si durante o período amostrado. Mas é pouco provável que tenha contribuído para aumentar a resistência do trigo à mancha marrom. Entretanto, na presença de Si, o decréscimo na concentração de CFST, em plantas supridas com Si, foi acompanhado com aumento na LATG e decréscimo na severidade da mancha marrom. Altas concentrações de LATG em plantas inoculadas e supridas com Si podem ter contribuído para aumento no nível de resistência das cultivares de trigo à mancha marrom. A atividade das QUI foi significativamente maior em plantas de ambas as cultivares supridas com Si a partir das 48 HAI. A atividade da POX foi maior apenas em plantas da cultivar BRS- 208 supridas com Si. Uma vez que plantas supridas com esse elemento apresentaram baixa severidade da doença a maior atividade das enzimas QUI e POX podendo indicar a participação dessas enzimas na defesa de plantas de trigo à mancha marrom. PFO, entretanto não teve sua atividade alterada em plantas supridas ou não com Si. Dos resultados deste estudo resultam as primeiras evidências de que o Si afeta a severidade da mancha marrom pela alteração de alguns componentes de resistência e pela potencialização de repostas de defesa fisiológicas e bioquímicas. Dessa forma, o Si tem grande potencial para ser usado como medida no manejo da mancha marrom do trigo no futuro.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleTrigo - Doenças e pragas - Aspectos fisiológicosTrigo - Doenças e pragas - Aspectos bioquímicosTrigo - Efeito do silícioBipolaris sorokinianaEpidemiologiaWheat - Diseases and pests - Physiological aspectsWheat - Diseases and pests - Biochemical aspectsWheat - Effect of siliconBipolaris sorokinianaEpidemiologyCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAEfeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicasSilicon effect on brown leaf spot development: resistance components and physiological and biochemical responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/embargoedAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf818538https://locus.ufv.br//bitstream/123456789/4435/1/texto%20completo.pdf5b4c34ab0c74c476bd9e6ed8a277268eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain116313https://locus.ufv.br//bitstream/123456789/4435/2/texto%20completo.pdf.txtc4debdf758feced97b2c1cb2f970b73dMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3555https://locus.ufv.br//bitstream/123456789/4435/3/texto%20completo.pdf.jpg1b1db5104b7218831925f4c69acdb681MD53123456789/44352016-04-10 23:10:18.655oai:locus.ufv.br:123456789/4435Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:10:18LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
dc.title.alternative.eng.fl_str_mv Silicon effect on brown leaf spot development: resistance components and physiological and biochemical responses
title Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
spellingShingle Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
Domiciano, Gisele Pereira
Trigo - Doenças e pragas - Aspectos fisiológicos
Trigo - Doenças e pragas - Aspectos bioquímicos
Trigo - Efeito do silício
Bipolaris sorokiniana
Epidemiologia
Wheat - Diseases and pests - Physiological aspects
Wheat - Diseases and pests - Biochemical aspects
Wheat - Effect of silicon
Bipolaris sorokiniana
Epidemiology
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
title_full Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
title_fullStr Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
title_full_unstemmed Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
title_sort Efeito do silício no desenvolvimento da mancha marrom do trigo: componentes de resistência e respostas fisiológicas e bioquímicas
author Domiciano, Gisele Pereira
author_facet Domiciano, Gisele Pereira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/0212901405730925
dc.contributor.author.fl_str_mv Domiciano, Gisele Pereira
dc.contributor.advisor-co1.fl_str_mv Rodrigues, Fabrício de ávila
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6
dc.contributor.advisor-co2.fl_str_mv Jesus Júnior, Waldir Cintra de
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737931T8
dc.contributor.advisor1.fl_str_mv Vale, Francisco Xavier Ribeiro do
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788182P7
dc.contributor.referee1.fl_str_mv Pereira, Olinto Liparini
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4
dc.contributor.referee2.fl_str_mv Dhingra, Onkar Dev
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788051H5
contributor_str_mv Rodrigues, Fabrício de ávila
Jesus Júnior, Waldir Cintra de
Vale, Francisco Xavier Ribeiro do
Pereira, Olinto Liparini
Dhingra, Onkar Dev
dc.subject.por.fl_str_mv Trigo - Doenças e pragas - Aspectos fisiológicos
Trigo - Doenças e pragas - Aspectos bioquímicos
Trigo - Efeito do silício
Bipolaris sorokiniana
Epidemiologia
topic Trigo - Doenças e pragas - Aspectos fisiológicos
Trigo - Doenças e pragas - Aspectos bioquímicos
Trigo - Efeito do silício
Bipolaris sorokiniana
Epidemiologia
Wheat - Diseases and pests - Physiological aspects
Wheat - Diseases and pests - Biochemical aspects
Wheat - Effect of silicon
Bipolaris sorokiniana
Epidemiology
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Wheat - Diseases and pests - Physiological aspects
Wheat - Diseases and pests - Biochemical aspects
Wheat - Effect of silicon
Bipolaris sorokiniana
Epidemiology
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The objective of this work was to evaluate the effect of silicon (Si) in some resistance components of wheat plants against brown leaf spot (BLS), caused by Bipolaris sorokiniana as well as, physiological and biochemical defence responses induced by this element. To do so, plants of two wheat cultivars, BR-18 and BRS- 208, were grown in pots containing 0 and 0.30 g Si/kg of soil and inoculated 45 days after emergency with B. sorokiniana. Incubation period (IP), mean lesion number by cm2 of leaf area (MLN), mean lesion size (MLS) and the area under the disease progress curve (AUDPC) were measured to evaluated silicon effect on resistance components. Photosynthetic rate (PR), total soluble phenolyc compounds concentration (TSPCC), lignin acid tioglycolic derivates concentration (LATDC) and enzymatic activity of quitinases (QUI), peroxidases (POX) and poliphenoloxidase (POD) were measured to evaluate if silicon could induce physiological and biochemical responses. It was verified in this study that silicon concentration was 90,5% higher in plants supplied with this element in comparison with control plants. Moreover, it was observed a higher level of silicon deposition on leafs of both cultivars supplied with this element was revealed by means of X-rays microanalysis. In these plants the IP was prolonged in near 10h in silicon supplied plants, possibly due to physical blocking effect that this element have on fungus penetration. It was also verified that in plant supplied with silicon this element reduced the MLN, the MLS and the AUDPC in 30%, 58% and 68%, respectively, in comparison with control plants. And more, silicon concentrations correlated positively with IP (r = 0.62) and negatively with AUDPC and MLS (r = -0.57 and -0.70, respectively). The resistance components of plants supplied with Si and inoculated with B. sorokiniana were different from those measured in plants inoculated e not supplied with this element. Since silicon was the only source of variation in the experiments it can be conclude that Si enhances resistance of wheat plants against spot brown. The PR was reduced in leafs and flag leafs of plants of both cultivars after 48 hours after inoculation (HAI). However, in plants supplied with silicon, the PR was kept higher during all evaluation, possibly due to low severity of BLS in these plants. Alterations in TSPCC and LATDC due to silicon effect were also observed. Even though TSPCC oscillated a bit, it was significantly lower in leafs of silicon supplied plants during the evaluation time, although it ́s unlike that it contributed for enhancing wheat resistance to BLS. However, in the presence of silicon the decrease in TSPCC was accompanied with increase of LATDC and decrease in BLS severity. Higher concentrations of LATD in plants supplied with silicon and inoculated with B. sorokiniana, may have contributed to enhancement of cultivars resistance against BLS. QUI activity was significantly enhanced in plants of both cultivars supplied with silicon after 48 HAI. POX activity was increased only in plants of cultivar BRS- 208 supplied with silicon. Since plants supplied with silicon showed low BSP severity the high activity of QUI and POX may indicate that these enzymes participates in wheat defense against BLS. PFO activity, however, did not significantly changed between plants supplied or not with silicon. The results of this study are first evidences that silicon affects BLS severity by altering wheat resistance components to BLS and by the potentialization of physiological and biochemical responses. Silicon has then, a great potential to be used as a BLS management measure in near future.
publishDate 2008
dc.date.issued.fl_str_mv 2008-02-21
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:54Z
dc.date.available.fl_str_mv 2015-03-06
2015-03-26T13:37:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv DOMICIANO, Gisele Pereira. Silicon effect on brown leaf spot development: resistance components and physiological and biochemical responses. 2008. 62 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4435
identifier_str_mv DOMICIANO, Gisele Pereira. Silicon effect on brown leaf spot development: resistance components and physiological and biochemical responses. 2008. 62 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
url http://locus.ufv.br/handle/123456789/4435
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dc.publisher.program.fl_str_mv Mestrado em Fitopatologia
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dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
publisher.none.fl_str_mv Universidade Federal de Viçosa
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