Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix

Detalhes bibliográficos
Autor(a) principal: Silva, Rosemeire Alves da
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4423
Resumo: Coffee is a major export product from Brazil having high importance for the country's economy. Thus, it becomes necessary to develop technologies to improve the Brazilian coffee farming. The main disease problem affecting coffee production is the coffee rust, a disease caused by the biotrophic fungus Hemileia vastratrix. The use of resistant varieties has been one of the main measures used to control the disease. One of the most used sources to obtain resistant varieties is the Híbrido Timor. Hence, the characterization the and understanding of the resistance of available accessions of Híbrido Timor to H. vastratrix are essential for the comprehension of the genetic basis of the resistance to this disease and to assist in the choice of genotypes to be used in breeding programs aimed at obtaining cultivars with durable resistance to rust. In this work, the germplasm bank of the Federal University of Viçosa, containing 152 accessions of Híbrido Timor and 6 genotypes from the EPAMIG breeding program were molecularly characterized and analyzed for resistance to two races of H. vastratrix, namely II and XXXIII. For the molecular characterization, 29 microsatellite primers were used and 92 bands were obtained with an average of 3.17 bands per primer. Of the 92 total bands obtained, 75 were polymorphic. Five primer combinations of AFLP were utilized by which 126 polymorphic bands were obtained with an average of 25.2 bands per combination of primer. Using only the data from microsatellite markers and the grouping based on the arithmetical complements of Jaccard and the UPGMA method, it was possible to separate 151 genotypes. Seven accessions did not differ, namely UFV 450-06, UFV450-12, UFV 428-01, UFV 428-02, UFV427-15, UFV427-90 and UFV428-04. By adding the data from the dominant markers AFLP, it was possible to separate these individuals, not only discriminating the accessions 427-90 and 428-04. It was possible to determine a unique molecular pattern (fingerprinting) for each individual using 58 marks. For the phenotypic characterization, the races II and XXXIII were inoculated in the152 accessions Híbrido Timor and 6 genotypes improving EPAMIG. All accessions were resistant to race II, which shows that the accessions may have either only one resistance gene, but not SH5, or more than one gene which may be any combination of SH 5, 6, 7, 8 e 9. With the race XXXIII, 14 accessions were susceptible. The race XXXIII presents more than one virulence gene which was able to overcome he resistance of the accessions UFV376-01, UFV376-02, UFV 376-08, UFV 376-09, UFV 376-14, UFV 376-37, UFV 377-51, UFV 408-10,UFV 408-18, UFV 432-30, UFV 445- 70, 2 II P6, 12 III P6 and 22 IV P6. This accessions contain the genes SH5,7 or SH 5,7 e 9. We conclude that 140 accessions of Híbrido Timor remain a good source of resistance to the races II and XXXIII and three materials from the EPAMIG/UFV program were susceptible the race XXXIII.
id UFV_b952b4ec695a1d7b12534ae740544908
oai_identifier_str oai:locus.ufv.br:123456789/4423
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Silva, Rosemeire Alves dahttp://lattes.cnpq.br/0296304382783168Zambolim, Laérciohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787254T6Pereira, Antonio Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780579Y1Caixeta, Eveline Teixeirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728636Z7Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D42015-03-26T13:37:52Z2014-10-062015-03-26T13:37:52Z2013-07-30SILVA, Rosemeire Alves da. Molecular characterization and resistance of Híbrido Timor coffee to Hemileia vastratrix. 2013. 66 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4423Coffee is a major export product from Brazil having high importance for the country's economy. Thus, it becomes necessary to develop technologies to improve the Brazilian coffee farming. The main disease problem affecting coffee production is the coffee rust, a disease caused by the biotrophic fungus Hemileia vastratrix. The use of resistant varieties has been one of the main measures used to control the disease. One of the most used sources to obtain resistant varieties is the Híbrido Timor. Hence, the characterization the and understanding of the resistance of available accessions of Híbrido Timor to H. vastratrix are essential for the comprehension of the genetic basis of the resistance to this disease and to assist in the choice of genotypes to be used in breeding programs aimed at obtaining cultivars with durable resistance to rust. In this work, the germplasm bank of the Federal University of Viçosa, containing 152 accessions of Híbrido Timor and 6 genotypes from the EPAMIG breeding program were molecularly characterized and analyzed for resistance to two races of H. vastratrix, namely II and XXXIII. For the molecular characterization, 29 microsatellite primers were used and 92 bands were obtained with an average of 3.17 bands per primer. Of the 92 total bands obtained, 75 were polymorphic. Five primer combinations of AFLP were utilized by which 126 polymorphic bands were obtained with an average of 25.2 bands per combination of primer. Using only the data from microsatellite markers and the grouping based on the arithmetical complements of Jaccard and the UPGMA method, it was possible to separate 151 genotypes. Seven accessions did not differ, namely UFV 450-06, UFV450-12, UFV 428-01, UFV 428-02, UFV427-15, UFV427-90 and UFV428-04. By adding the data from the dominant markers AFLP, it was possible to separate these individuals, not only discriminating the accessions 427-90 and 428-04. It was possible to determine a unique molecular pattern (fingerprinting) for each individual using 58 marks. For the phenotypic characterization, the races II and XXXIII were inoculated in the152 accessions Híbrido Timor and 6 genotypes improving EPAMIG. All accessions were resistant to race II, which shows that the accessions may have either only one resistance gene, but not SH5, or more than one gene which may be any combination of SH 5, 6, 7, 8 e 9. With the race XXXIII, 14 accessions were susceptible. The race XXXIII presents more than one virulence gene which was able to overcome he resistance of the accessions UFV376-01, UFV376-02, UFV 376-08, UFV 376-09, UFV 376-14, UFV 376-37, UFV 377-51, UFV 408-10,UFV 408-18, UFV 432-30, UFV 445- 70, 2 II P6, 12 III P6 and 22 IV P6. This accessions contain the genes SH5,7 or SH 5,7 e 9. We conclude that 140 accessions of Híbrido Timor remain a good source of resistance to the races II and XXXIII and three materials from the EPAMIG/UFV program were susceptible the race XXXIII.O café representa um dos principais produtos de exportação do Brasil possuindo elevada importância para a economia do país. Dessa forma, torna-se necessário o desenvolvimento de tecnologias que visam melhorar a cafeicultura brasileira. O principal problema fitossanitário que afeta a produção do café é a ferrugem, doença causada pelo fungo biotrófico Hemileia vastatrix. O uso de variedades resistentes tem sido uma das principais medidas utilizadas para controle da doença. Uma das fontes de resistência mais utilizada para obtenção das variedades resistentes é o Híbrido de Timor. Dessa forma, a caracterização e o conhecimento da resistência dos acessos de Híbrido de Timor a H. vastatrix disponíveis é essencial para o entendimento da base genética da resistência a essa doença e para auxiliar na escolha de genótipos para serem usados nos programas de melhoramento que visam a obtenção de cultivares com resistência durável à ferrugem. Neste trabalho, o banco de germoplasma da Universidade Federal de Viçosa, contendo 152 acessos de Híbridos de Timor e 6 genótipos do programa de melhoramento da EPAMIG foram caracterizados molecularmente e analisados quanto a resistência a duas raças de H. vastatrix raça II e XXXIII. Na caracterização molecular foram utilizados 29 primers microssatélites sendo obtidas 92 bandas com média de 3,17 bandas por primer. Das 92 bandas totais obtidas 75 foram polimórficas, foram utilizadas 5 combinações de primers AFLP onde foram obtidos 126 bandas polimórficas com média de 25,2 bandas por combinação de primer. Utilizando apenas os dados dos marcadores microssatélites e o agrupamento baseado nos complementos aritméticos de Jaccard e o método UPGMA foi possível separar 151 genótipos. Sete acessos não diferiram, sendo eles UFV450-06, UFV450-12, UFV428-01, UFV428-02, UFV427,15, UFV427-90 e UFV428-04. Ao acrescentar os dados dos marcadores dominantes AFLP foi possível separar estes indivíduos, não discriminando apenas os acessos UFV427-90 e UFV428-04. Foi possível determinar um padrão molecular único (fingerpinting) para cada indivíduo utilizando 58 marcas. Na caracterização fenotípica foram inoculadas às raças II e XXXIII nos 152 acessos Híbrido de Timor e 6 genótipos do melhoramento EPAMIG. Todos os acessos apresentaram resistência à raça II, o que mostra que os acessos podem ter apenas um gene de resistência que não o SH 5 ou mais de um gene, sendo estes qualquer combinação dos genes SH 5, 6, 7, 8 e 9. Com a raça XXXIII, 14 acessos apresentaram suscetibilidade, sendo a raça XXXIII com mais de um gene de virulência que foi capaz de suplantar a resistência dos acessos UFV376-01, UFV376-02, UFV 376-08, UFV 376-09, UFV 376-14, UFV 376-37, UFV 377-51, UFV 408-10,UFV 408-18, UFV 432-30, UFV 445-70, 2 II P6, 12 III P6 e 22 IV P6, estes acessos contém os genes SH 5, 7 ou SH 5,7 e 9. Conclui-se que 140 acessos de Híbridos de Timor continuam sendo uma boa fonte de resistência às raças II e XXXIII e três material do programa de melhoramento EPAMIG/UFV foram suscetível a raça XXXIII.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleResistênciaMarcador molecularBanco de germoplasmaResistanceMolecular markerGenebankCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIACaracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrixMolecular characterization and resistance of Híbrido Timor coffee to Hemileia vastratrixinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf979876https://locus.ufv.br//bitstream/123456789/4423/1/texto%20completo.pdf477fe512a1a7345a18a1012521320562MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain112134https://locus.ufv.br//bitstream/123456789/4423/2/texto%20completo.pdf.txt6fd9f6753fa50e585b70f49e93e3941dMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3528https://locus.ufv.br//bitstream/123456789/4423/3/texto%20completo.pdf.jpga561ca6c39b93003b5a08a67e755d0e0MD53123456789/44232016-04-10 23:09:40.929oai:locus.ufv.br:123456789/4423Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:09:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
dc.title.alternative.eng.fl_str_mv Molecular characterization and resistance of Híbrido Timor coffee to Hemileia vastratrix
title Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
spellingShingle Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
Silva, Rosemeire Alves da
Resistência
Marcador molecular
Banco de germoplasma
Resistance
Molecular marker
Genebank
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
title_full Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
title_fullStr Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
title_full_unstemmed Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
title_sort Caracterização molecular e resistência do cafeeiro Híbrido de Timor à Hemileia vastatrix
author Silva, Rosemeire Alves da
author_facet Silva, Rosemeire Alves da
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/0296304382783168
dc.contributor.author.fl_str_mv Silva, Rosemeire Alves da
dc.contributor.advisor1.fl_str_mv Zambolim, Laércio
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787254T6
dc.contributor.referee1.fl_str_mv Pereira, Antonio Alves
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780579Y1
dc.contributor.referee2.fl_str_mv Caixeta, Eveline Teixeira
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728636Z7
dc.contributor.referee3.fl_str_mv Pereira, Olinto Liparini
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4
contributor_str_mv Zambolim, Laércio
Pereira, Antonio Alves
Caixeta, Eveline Teixeira
Pereira, Olinto Liparini
dc.subject.por.fl_str_mv Resistência
Marcador molecular
Banco de germoplasma
topic Resistência
Marcador molecular
Banco de germoplasma
Resistance
Molecular marker
Genebank
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Resistance
Molecular marker
Genebank
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description Coffee is a major export product from Brazil having high importance for the country's economy. Thus, it becomes necessary to develop technologies to improve the Brazilian coffee farming. The main disease problem affecting coffee production is the coffee rust, a disease caused by the biotrophic fungus Hemileia vastratrix. The use of resistant varieties has been one of the main measures used to control the disease. One of the most used sources to obtain resistant varieties is the Híbrido Timor. Hence, the characterization the and understanding of the resistance of available accessions of Híbrido Timor to H. vastratrix are essential for the comprehension of the genetic basis of the resistance to this disease and to assist in the choice of genotypes to be used in breeding programs aimed at obtaining cultivars with durable resistance to rust. In this work, the germplasm bank of the Federal University of Viçosa, containing 152 accessions of Híbrido Timor and 6 genotypes from the EPAMIG breeding program were molecularly characterized and analyzed for resistance to two races of H. vastratrix, namely II and XXXIII. For the molecular characterization, 29 microsatellite primers were used and 92 bands were obtained with an average of 3.17 bands per primer. Of the 92 total bands obtained, 75 were polymorphic. Five primer combinations of AFLP were utilized by which 126 polymorphic bands were obtained with an average of 25.2 bands per combination of primer. Using only the data from microsatellite markers and the grouping based on the arithmetical complements of Jaccard and the UPGMA method, it was possible to separate 151 genotypes. Seven accessions did not differ, namely UFV 450-06, UFV450-12, UFV 428-01, UFV 428-02, UFV427-15, UFV427-90 and UFV428-04. By adding the data from the dominant markers AFLP, it was possible to separate these individuals, not only discriminating the accessions 427-90 and 428-04. It was possible to determine a unique molecular pattern (fingerprinting) for each individual using 58 marks. For the phenotypic characterization, the races II and XXXIII were inoculated in the152 accessions Híbrido Timor and 6 genotypes improving EPAMIG. All accessions were resistant to race II, which shows that the accessions may have either only one resistance gene, but not SH5, or more than one gene which may be any combination of SH 5, 6, 7, 8 e 9. With the race XXXIII, 14 accessions were susceptible. The race XXXIII presents more than one virulence gene which was able to overcome he resistance of the accessions UFV376-01, UFV376-02, UFV 376-08, UFV 376-09, UFV 376-14, UFV 376-37, UFV 377-51, UFV 408-10,UFV 408-18, UFV 432-30, UFV 445- 70, 2 II P6, 12 III P6 and 22 IV P6. This accessions contain the genes SH5,7 or SH 5,7 e 9. We conclude that 140 accessions of Híbrido Timor remain a good source of resistance to the races II and XXXIII and three materials from the EPAMIG/UFV program were susceptible the race XXXIII.
publishDate 2013
dc.date.issued.fl_str_mv 2013-07-30
dc.date.available.fl_str_mv 2014-10-06
2015-03-26T13:37:52Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Rosemeire Alves da. Molecular characterization and resistance of Híbrido Timor coffee to Hemileia vastratrix. 2013. 66 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4423
identifier_str_mv SILVA, Rosemeire Alves da. Molecular characterization and resistance of Híbrido Timor coffee to Hemileia vastratrix. 2013. 66 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.
url http://locus.ufv.br/handle/123456789/4423
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.publisher.program.fl_str_mv Mestrado em Fitopatologia
dc.publisher.initials.fl_str_mv UFV
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/4423/1/texto%20completo.pdf
https://locus.ufv.br//bitstream/123456789/4423/2/texto%20completo.pdf.txt
https://locus.ufv.br//bitstream/123456789/4423/3/texto%20completo.pdf.jpg
bitstream.checksum.fl_str_mv 477fe512a1a7345a18a1012521320562
6fd9f6753fa50e585b70f49e93e3941d
a561ca6c39b93003b5a08a67e755d0e0
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1801213010685984768