Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia).
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4315 |
Resumo: | The use of cytokinins in variable concentrations in in vitro plant tissue culture can induce the occurrence of structural and morphological changes, affecting the development and viability of the obtained material. The objective of this work was to characterize the influence of 6-benzylaminopurine (BAP) upon morphogenesis and mineral nutrition of grape rootstock VR043-43 (Vitis vinifera x Vitis rotundifolia). Plants obtained from nodal segments in hyperhydricity and callogenesis media, supplemented with BAP and without regulator were used. In callogenesis induction medium plants fresh and dry weight, macro and micronutrients contents, oxygen and carbon dioxide levels in the headspace were measured at 15, 30 and 45 days. In hyperhydricity induction medium, the levels of macro and micronutrients and structural analysis of the leaves obtained at 45 days. The results demonstrated that BAP organogenesis, promoting callogenesis and inhibited rhizogenesis, internodal elongation and leaf expansion. BAP also altered the relation of fresh and dry mass of shoot/ root system in detriment to shoots; altered the nutrition of the shoots, inducing callus formation, which acted as a mechanical barrier to elements absorption from the medium. There was higher accumulation of CO2 and consumption of O2 in callogenesis induction medium, in flasks with cytokinin, reflecting the elevated respiratory rate of the calluses, which prejudiced growth and development. In hyperhydricity induction medium, BAP promoted alterations in grape plants morphology, as multiple shoots, leaf lamina thickness and callusing in the base of the explants; and structural alterations, such as disorganization of the mesophyll parenchyma cells and stomata differentiated above the other epidermic cells. Leaves developed in medium with BAP did not accumulated starch, however their vascular bundles were most lignified and there was higher accumulation of green coloration compounds in reaction with toluidine blue concomitant with increasing of cytokinin concentration. We conclude that BAP promoted alterations in morphogenesis and development of the plants. The calluses formed in BAP-containing medium promoted alterations in nutrients absorption and translocation to shoots, as also in respiratory rates. There was hyperhydricity induction by BAP, which was evidenced by morphologic changes and structural alterations in leaves and in guard-cells shape and disposition. |
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Fernandes, Katryne Rates Goularthttp://lattes.cnpq.br/2567037472204692Silva, Luzimar Campos dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8Otoni, Wagner Camposhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y6Costa, Franciscleudo Bezerra dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765235U9Ribeiro, Dimas Mendeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778644J9Buselli, Reginaldo Alves Festuccihttp://lattes.cnpq.br/87118297194428652015-03-26T13:36:40Z2010-11-122015-03-26T13:36:40Z2010-03-31FERNANDES, Katryne Rates Goulart. Influence of 6-benzylaminopurine on in vitro morphogenesis and hyperhydricity occurrence in VR043-43 (Vitis vinifera x V. rotundifolia) rootstock.. 2010. 66 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/4315The use of cytokinins in variable concentrations in in vitro plant tissue culture can induce the occurrence of structural and morphological changes, affecting the development and viability of the obtained material. The objective of this work was to characterize the influence of 6-benzylaminopurine (BAP) upon morphogenesis and mineral nutrition of grape rootstock VR043-43 (Vitis vinifera x Vitis rotundifolia). Plants obtained from nodal segments in hyperhydricity and callogenesis media, supplemented with BAP and without regulator were used. In callogenesis induction medium plants fresh and dry weight, macro and micronutrients contents, oxygen and carbon dioxide levels in the headspace were measured at 15, 30 and 45 days. In hyperhydricity induction medium, the levels of macro and micronutrients and structural analysis of the leaves obtained at 45 days. The results demonstrated that BAP organogenesis, promoting callogenesis and inhibited rhizogenesis, internodal elongation and leaf expansion. BAP also altered the relation of fresh and dry mass of shoot/ root system in detriment to shoots; altered the nutrition of the shoots, inducing callus formation, which acted as a mechanical barrier to elements absorption from the medium. There was higher accumulation of CO2 and consumption of O2 in callogenesis induction medium, in flasks with cytokinin, reflecting the elevated respiratory rate of the calluses, which prejudiced growth and development. In hyperhydricity induction medium, BAP promoted alterations in grape plants morphology, as multiple shoots, leaf lamina thickness and callusing in the base of the explants; and structural alterations, such as disorganization of the mesophyll parenchyma cells and stomata differentiated above the other epidermic cells. Leaves developed in medium with BAP did not accumulated starch, however their vascular bundles were most lignified and there was higher accumulation of green coloration compounds in reaction with toluidine blue concomitant with increasing of cytokinin concentration. We conclude that BAP promoted alterations in morphogenesis and development of the plants. The calluses formed in BAP-containing medium promoted alterations in nutrients absorption and translocation to shoots, as also in respiratory rates. There was hyperhydricity induction by BAP, which was evidenced by morphologic changes and structural alterations in leaves and in guard-cells shape and disposition.A utilização de citocininas no cultivo in vitro de plantas pode induzir, em variadas concentrações, a ocorrência de alterações estruturais e morfológicas, comprometendo o desenvolvimento e viabilidade do material obtido. Este trabalho objetivou caracterizar a influência da 6-benzilaminopurina (BAP) na morfogênese e nutrição do porta-enxerto de videira VR043-43 (Vitis vinifera x Vitis rotundifolia). Utilizou-se plantas obtidas a partir do cultivo de segmentos nodais em meio indutor de hiperidricidade e em meio indutor de calogênese, suplementados com BAP, e em meio sem regulador. Em meio indutor de calogênese foram mensurados a massa fresca e seca das plantas, os teores de macro e micronutrientes e os níveis de oxigênio e dióxido de carbono na atmosfera dos frascos aos 15, 30 e 45 dias. Em meio indutor de hiperidricidade determinou-se os teores de macro e micronutrientes aos 45 dias de cultivo, além da análise estrutural das folhas obtidas. Os resultados demonstraram que o BAP comprometeu a organogênese, promovendo calogênese e inibindo a rizogênese, o alongamento dos entrenós e a expansão foliar. Além disso, o BAP alterou a relação da massa fresca e seca da parte aérea/sistema radicular em detrimento dos ramos; alterou a nutrição da parte aérea das plantas, induzindo a formação de calos, os quais representaram uma barreira mecânica à absorção dos elementos do meio de cultivo. Houve maior acúmulo de CO2 e consumo de O2 na atmosfera dos frascos com citocinina, em meio indutor de calogênese, refletindo a elevada taxa respiratória dos calos, o que comprometeu o crescimento e o desenvolvimento. Em meio indutor de hiperidricidade, o BAP promoveu alterações na morfologia das plantas de videira, como múltiplas brotações, espessamento da lâmina foliar e calejamento na base dos explantes; e alterações estruturais, como desorganização das células parenquimáticas no mesofilo e estômatos dispostos acima das demais células da epiderme. As folhas desenvolvidas em meio contendo BAP não acumularam amido em relação ao controle, no entanto houve maior lignificação nos feixes vasculares e maior acúmulo de compostos de coloração esverdeada em reação com azul de toluidina, concomitante ao aumento na concentração desta citocinina. Conclui-se que o BAP promoveu alterações na morfogênese e no desenvolvimento das plantas. Os calos formados em meio com BAP promoveram alteração da absorção e translocação de nutrientes para a parte aérea das plantas, bem como alteração das taxas respiratórias. Houve indução de hiperidricidade pelo BAP, evidenciada por alterações morfológicas, e alteração estrutural das folhas e da forma e disposição das células-guarda.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Fisiologia VegetalUFVBRControle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superioresMorfogêneseHiperidricidadeVideiraMorphogenesisHyperhydricityVineCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALInfluência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia).Influence of 6-benzylaminopurine on in vitro morphogenesis and hyperhydricity occurrence in VR043-43 (Vitis vinifera x V. rotundifolia) rootstock.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf971229https://locus.ufv.br//bitstream/123456789/4315/1/texto%20completo.pdf1ce48be896b7f0a4e5e3235dedd600dcMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain102698https://locus.ufv.br//bitstream/123456789/4315/2/texto%20completo.pdf.txt7b12b6e574dc55956199507f7fe5357fMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3722https://locus.ufv.br//bitstream/123456789/4315/3/texto%20completo.pdf.jpg8cdad4b8c9e4b38d2045d8d1826d2e58MD53123456789/43152016-04-10 23:07:29.378oai:locus.ufv.br:123456789/4315Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:07:29LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
dc.title.alternative.eng.fl_str_mv |
Influence of 6-benzylaminopurine on in vitro morphogenesis and hyperhydricity occurrence in VR043-43 (Vitis vinifera x V. rotundifolia) rootstock. |
title |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
spellingShingle |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). Fernandes, Katryne Rates Goulart Morfogênese Hiperidricidade Videira Morphogenesis Hyperhydricity Vine CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
title_full |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
title_fullStr |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
title_full_unstemmed |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
title_sort |
Influência de 6-benzilaminopurina na morfogênese in vitro e na ocorrência de hiperidricidade no porta-enxerto VR043-43 (Vitis vinifera x Vitis rotundifolia). |
author |
Fernandes, Katryne Rates Goulart |
author_facet |
Fernandes, Katryne Rates Goulart |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2567037472204692 |
dc.contributor.author.fl_str_mv |
Fernandes, Katryne Rates Goulart |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Luzimar Campos da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8 |
dc.contributor.advisor1.fl_str_mv |
Otoni, Wagner Campos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y6 |
dc.contributor.referee1.fl_str_mv |
Costa, Franciscleudo Bezerra da |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765235U9 |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Dimas Mendes |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778644J9 |
dc.contributor.referee3.fl_str_mv |
Buselli, Reginaldo Alves Festucci |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8711829719442865 |
contributor_str_mv |
Silva, Luzimar Campos da Otoni, Wagner Campos Costa, Franciscleudo Bezerra da Ribeiro, Dimas Mendes Buselli, Reginaldo Alves Festucci |
dc.subject.por.fl_str_mv |
Morfogênese Hiperidricidade Videira |
topic |
Morfogênese Hiperidricidade Videira Morphogenesis Hyperhydricity Vine CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Morphogenesis Hyperhydricity Vine |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
The use of cytokinins in variable concentrations in in vitro plant tissue culture can induce the occurrence of structural and morphological changes, affecting the development and viability of the obtained material. The objective of this work was to characterize the influence of 6-benzylaminopurine (BAP) upon morphogenesis and mineral nutrition of grape rootstock VR043-43 (Vitis vinifera x Vitis rotundifolia). Plants obtained from nodal segments in hyperhydricity and callogenesis media, supplemented with BAP and without regulator were used. In callogenesis induction medium plants fresh and dry weight, macro and micronutrients contents, oxygen and carbon dioxide levels in the headspace were measured at 15, 30 and 45 days. In hyperhydricity induction medium, the levels of macro and micronutrients and structural analysis of the leaves obtained at 45 days. The results demonstrated that BAP organogenesis, promoting callogenesis and inhibited rhizogenesis, internodal elongation and leaf expansion. BAP also altered the relation of fresh and dry mass of shoot/ root system in detriment to shoots; altered the nutrition of the shoots, inducing callus formation, which acted as a mechanical barrier to elements absorption from the medium. There was higher accumulation of CO2 and consumption of O2 in callogenesis induction medium, in flasks with cytokinin, reflecting the elevated respiratory rate of the calluses, which prejudiced growth and development. In hyperhydricity induction medium, BAP promoted alterations in grape plants morphology, as multiple shoots, leaf lamina thickness and callusing in the base of the explants; and structural alterations, such as disorganization of the mesophyll parenchyma cells and stomata differentiated above the other epidermic cells. Leaves developed in medium with BAP did not accumulated starch, however their vascular bundles were most lignified and there was higher accumulation of green coloration compounds in reaction with toluidine blue concomitant with increasing of cytokinin concentration. We conclude that BAP promoted alterations in morphogenesis and development of the plants. The calluses formed in BAP-containing medium promoted alterations in nutrients absorption and translocation to shoots, as also in respiratory rates. There was hyperhydricity induction by BAP, which was evidenced by morphologic changes and structural alterations in leaves and in guard-cells shape and disposition. |
publishDate |
2010 |
dc.date.available.fl_str_mv |
2010-11-12 2015-03-26T13:36:40Z |
dc.date.issued.fl_str_mv |
2010-03-31 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:36:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, Katryne Rates Goulart. Influence of 6-benzylaminopurine on in vitro morphogenesis and hyperhydricity occurrence in VR043-43 (Vitis vinifera x V. rotundifolia) rootstock.. 2010. 66 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2010. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4315 |
identifier_str_mv |
FERNANDES, Katryne Rates Goulart. Influence of 6-benzylaminopurine on in vitro morphogenesis and hyperhydricity occurrence in VR043-43 (Vitis vinifera x V. rotundifolia) rootstock.. 2010. 66 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2010. |
url |
http://locus.ufv.br/handle/123456789/4315 |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Fisiologia Vegetal |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
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Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores |
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Universidade Federal de Viçosa |
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