Efeitos da doxorrubicina e tamoxifeno em camundongos Balb/c machos inoculados com tumor ascítico de Ehrlich

• Autor(a) principal: Calian, Odilon Azevedo
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5151
• topic: Achados histopatológicos; Doxorrubicina; Epirrubicina; Câncer; Histopathological findings, Doxorubicin; Epirubicin; Cancer; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL::ANATOMIA PATOLOGIA ANIMAL

Some drugs, such as doxorubicin, among other mechanisms act by triggering apoptosis, which not only affects the tumor but also normal cells in the body. Therefore, the study of the side effects of chemotherapy, seeking a less aggressive treatment for cancer patients, justifies this work. The objective was to investigate the hematological and gastrointestinal effects of doxorubicin and tamoxifen when used alone or in combination in male mice with or without the Ehrlich ascites tumor, with or without hormone therapy with estradiol cypionate. A total of 40 Balb / C male mice were divided into groups of 5 animals each, making 8 groups. At the end of 21 days, blood samples were collected for CBC (Complete Blood Count), aiming at evaluating parameters such as RBC count and hematocrit, hemoglobin, white blood cells count and platelets count. Samples of the stomach and intestines of the animals treated with hydrochloride doxorubicin, were also collected for histopathologic analysis, evaluating when present, the degree of damage to the epithelium and the degree of necrosis and apoptosis glands. The evaluation of the number of erythrocytes and the hematocrit value was significantly decreased (p <0.05) in groups I and VIII when compared to the other groups. Hemoglobin showed a significant decrease in group I in relation to groups II, III, IV and V. Counting the number of leukocytes showed a significant increase (p <0.05) when comparing groups I and VI with the other groups. The main histopathological changes evidenced in the stomach and small intestine were necrosis and apoptosis and loss of glands lining epithelium, and the group VII showed a slight decrease in the degree of lesions in group V.