Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/447 |
Resumo: | The incidence of obesity has been drastically increasing, reaching epidemic proportions worldwide, mainly in Western and industrialized countries. Scientific evidence relates obesity to a chronic inflammatory process of low intensity, due to increased pro-inflammatory cytokines, such as an abnormal production of tumor necrosis factor alpha (TNFα). Hypertrophy and hyperplasia of adipocytes have been associated with diseases related to the metabolic syndrome, in which obesity seems to be the pivot. Therefore, the study of adipogenesis is crucial in the understanding of obesity, as well as its treatment. Studies suggest that both the eicosapentaenoic acid (EPA) and medium-chain fatty acids (MCFA) are capable of modulating the incorporation of lipids during the adipogenesis and of regulating the expression of key adipogenic transcription factors to cell differentiation. This study aimed to evaluate the effect of EPA and MCFA (C8:0 and C12:0) over the adipogenesis in 3T3-L1 cells. Moreover, the effect of such fatty acids over the mRNA expression and chemerin secretion, a new adipocyte involved in adipogenesis, was evaluated. Hence, preadipocytes were cultured in the presence (induced adipogenesis) or absence (spontaneous adipogenesis) of a hormonal cocktail. Throughout the differentiation process, cells were treated with C8:0 (250 μM), C12:0 (250 μM) and EPA (100 or 200 μM), supplemented or not with TNFα. During the experimental period, samples were collected in order to assess the gene expression of: preadipogenenic factor (DLK1), wingless related MMTV integration site 10b (WNT10b); CCAAT / enhancer-binding proteins (C/EBP) β, δ, and α; peroxisome proliferator-activated receptor gamma (PPARγ) and chemerin. The incorporation of lipids was determined by Oil Red-O. Furthermore, still regarding the study of chemerin, mature adipocytes were incubated for 24 hours with the previously described fatty acids, along with TNFα and insulin, in the presence or absence of protein kinases inhibitors. At the end of this period, an analysis of the mRNA expression and chemerin secretion was conducted. The results showed that during the induced adipogenesis, the fatty acids did not significantly alter the incorporation of lipids. On the other hand, during the spontaneous adipogenesis, EPA (200 μM) promoted a greater incorporation of lipids (p<0.05), while the tested MCFA demonstrated no such effect. When added of TNFα, the C8:0 decreased the incorporation of lipids during the induced adipogenesis, thus enhancing the inhibitory effect of this cytokine. When added of TNFα, EPA stimulated the mRNA expression of the DLK1, WNT10b and C/EBPβ, whereas the C/EBPα and PPARγ expression were inhibited. The EPA did not present significant effects over the C/EBPδ. The MCFA were able to regulate the expression of the classical transcriptional adipogenic factors (C/EPBβ, α and δ and PPARγ), with particular emphasis given to the effects achieved by the C8:0. Regarding the expression of chemerin, it was observed that, apparently, the studied fatty acids did not participate in the secretion and expression of such adipokine. However, in a 24-hour incubation study, TNFα stimulated the expression of the mRNA chemerin, as well as its secretion, possibly via phosphatidil inositol 3-kinase (PI3K). In conclusion, the results showed that the analyzed fatty acids are capable of modulating the incorporation of lipids, as well as the expression of key adipogenic transcription factors, thus potentializing the effect of the TNFα. |
id |
UFV_bf30f7626b51f7f28af116f39b1388de |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/447 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Costa, André Gustavo Vasconceloshttp://lattes.cnpq.br/5406130298857736Ribeiro, Sônia Machado Rochahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701461E0Silva, Fabyano Fonseca ehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766260Z2Bressan, Josefinahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781728Y2Aliaga, Maria Jesus MorenoPaula, Sérgio Oliveira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P42015-03-26T12:25:04Z2012-04-022015-03-26T12:25:04Z2010-06-21COSTA, André Gustavo Vasconcelos. Regulation of adipogenesis and chemerin secretion by medium-chain fatty acids and eicosapentaenoic acid in 3T3-L1 cells. 2010. 118 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/447The incidence of obesity has been drastically increasing, reaching epidemic proportions worldwide, mainly in Western and industrialized countries. Scientific evidence relates obesity to a chronic inflammatory process of low intensity, due to increased pro-inflammatory cytokines, such as an abnormal production of tumor necrosis factor alpha (TNFα). Hypertrophy and hyperplasia of adipocytes have been associated with diseases related to the metabolic syndrome, in which obesity seems to be the pivot. Therefore, the study of adipogenesis is crucial in the understanding of obesity, as well as its treatment. Studies suggest that both the eicosapentaenoic acid (EPA) and medium-chain fatty acids (MCFA) are capable of modulating the incorporation of lipids during the adipogenesis and of regulating the expression of key adipogenic transcription factors to cell differentiation. This study aimed to evaluate the effect of EPA and MCFA (C8:0 and C12:0) over the adipogenesis in 3T3-L1 cells. Moreover, the effect of such fatty acids over the mRNA expression and chemerin secretion, a new adipocyte involved in adipogenesis, was evaluated. Hence, preadipocytes were cultured in the presence (induced adipogenesis) or absence (spontaneous adipogenesis) of a hormonal cocktail. Throughout the differentiation process, cells were treated with C8:0 (250 μM), C12:0 (250 μM) and EPA (100 or 200 μM), supplemented or not with TNFα. During the experimental period, samples were collected in order to assess the gene expression of: preadipogenenic factor (DLK1), wingless related MMTV integration site 10b (WNT10b); CCAAT / enhancer-binding proteins (C/EBP) β, δ, and α; peroxisome proliferator-activated receptor gamma (PPARγ) and chemerin. The incorporation of lipids was determined by Oil Red-O. Furthermore, still regarding the study of chemerin, mature adipocytes were incubated for 24 hours with the previously described fatty acids, along with TNFα and insulin, in the presence or absence of protein kinases inhibitors. At the end of this period, an analysis of the mRNA expression and chemerin secretion was conducted. The results showed that during the induced adipogenesis, the fatty acids did not significantly alter the incorporation of lipids. On the other hand, during the spontaneous adipogenesis, EPA (200 μM) promoted a greater incorporation of lipids (p<0.05), while the tested MCFA demonstrated no such effect. When added of TNFα, the C8:0 decreased the incorporation of lipids during the induced adipogenesis, thus enhancing the inhibitory effect of this cytokine. When added of TNFα, EPA stimulated the mRNA expression of the DLK1, WNT10b and C/EBPβ, whereas the C/EBPα and PPARγ expression were inhibited. The EPA did not present significant effects over the C/EBPδ. The MCFA were able to regulate the expression of the classical transcriptional adipogenic factors (C/EPBβ, α and δ and PPARγ), with particular emphasis given to the effects achieved by the C8:0. Regarding the expression of chemerin, it was observed that, apparently, the studied fatty acids did not participate in the secretion and expression of such adipokine. However, in a 24-hour incubation study, TNFα stimulated the expression of the mRNA chemerin, as well as its secretion, possibly via phosphatidil inositol 3-kinase (PI3K). In conclusion, the results showed that the analyzed fatty acids are capable of modulating the incorporation of lipids, as well as the expression of key adipogenic transcription factors, thus potentializing the effect of the TNFα.A incidência da obesidade vem aumentando drasticamente atingindo proporções epidêmicas no mundo, principalmente nos países ocidentais e industrializados. Evidências científicas relacionam a obesidade a um processo inflamatório crônico de baixa intensidade, devido ao aumento de citocinas proinflamatórias, como a produção anormal de fator de necrose tumoral alfa (TNFα). A hipertrofia e a hiperplasia do adipócito têm sido associadas às doenças relacionadas à síndrome metabólica, na qual a obesidade parece ser o pivô. Assim, o estudo da adipogênese é fundamental para o entendimento da obesidade, bem como para seu tratamento. Estudos sugerem que o ácido eicosapentaenóico (EPA), bem como os ácidos graxos de cadeia média (AGCM) são capazes de modular a incorporação de lipídios durante a adipogênese e de regular a expressão de fatores transcricionais chave da diferenciação celular. Este estudo objetivou avaliar o efeito do EPA e de AGCM (C8:0 e C12:0) sobre a adipogênese em células 3T3-L1. Além disso, avaliou-se o efeito desses ácidos graxos sobre a expressão de mRNA e secreção de quemerina, uma nova adipocina envolvida na adipogênese. Para isso, preadipócitos foram cultivados em presença (adipogênese induzida) ou ausência (adipogênese espontânea) de coquetel de hormônios. Durante o processo de diferenciação, as células foram tratadas com C8:0 (250 μM), C12:0 (250 μM) e EPA (100 ou 200 μM), acrescidos ou não de TNFα. Durante o período experimental foram coletadas amostras para avaliar a expressão gênica de: fator preadipogênico (DLK1); Wingless related MMTV integration site 10b (WNT10b); proteínas ligantes ao amplificador CCAAT (C/EBP) β, δ e α; PPARγ e quemerina. A incorporação de lipídios foi avaliada por Oil Red-O. Ainda, para o estudo sobre quemerina, adipócitos maduros foram incubados por 24 horas com os ácidos graxos descritos anteriormente, bem como com TNFα e insulina, em presença ou ausência de inibidores de proteínas quinase. Ao final desse período efetuou-se a análise de expressão de mRNA e da secreção de quemerina. Os resultados mostraram que durante a adipogênese induzida os ácidos graxos não alteraram significativamente a incorporação de lipídios. Por outro lado, o EPA (200 μM), durante a adipogênese espontânea, promoveu maior incorporação de lipídios (p<0,05), ao passo que os AGCM testados não apresentaram esse efeito. O C8:0 diminuiu a incorporação de lipídios durante a adipogênese induzida, quando acrescido de TNFα, ampliando o efeito inibitório dessa citocina. O EPA, acrescido de TNFα, estimulou a expressão de mRNA de DLK1, de WNT10b e de C/EBPβ, porém inibiram a expressão de C/EBPα e PPARγ. O EPA não apresentou efeitos significativos sobre C/EBPδ. Os AGCM foram capazes de regular a expressão de fatores adipogênicos transcricionais clássicos (C/EPBβ, δ e α e PPARγ), com destaque especial aos efeitos desempenhados pelo C8:0. Em relação à expressão de quemerina, observou-se que os ácidos graxos testados aparentemente não participam da secreção e expressão dessa adipocina. Entretanto, no estudo de 24 horas de incubação, o TNFα estimulou a expressão de mRNA de quemerina, bem como sua secreção, possivelmente via fosfatidil inositol 3 quinase (PI3K). Em conjunto, os resultados mostraram que os ácidos graxos testados são capazes de modular a incorporação de lipídios, bem como a expressão de fatores transcricionais chave da diferenciação, potencializando o efeito do TNFα.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em Ciência e Tecnologia de AlimentosUFVBRCiência de Alimentos; Tecnologia de Alimentos; Engenharia de AlimentosLipídiosSíndrome metabólicaAdipocinasLipidsMetabolic syndromeAdipokinesCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSRegulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1Regulation of adipogenesis and chemerin secretion by medium-chain fatty acids and eicosapentaenoic acid in 3T3-L1 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2480580https://locus.ufv.br//bitstream/123456789/447/1/texto%20completo.pdfb17d37b14c0a089284fa76e6f70a912aMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain196929https://locus.ufv.br//bitstream/123456789/447/2/texto%20completo.pdf.txtec14cc91882d444a9c85bf290e89267bMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3541https://locus.ufv.br//bitstream/123456789/447/3/texto%20completo.pdf.jpgbdf6a30bade8fb621319c82219b1f4aeMD53123456789/4472016-04-06 23:05:02.524oai:locus.ufv.br:123456789/447Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:05:02LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
dc.title.alternative.eng.fl_str_mv |
Regulation of adipogenesis and chemerin secretion by medium-chain fatty acids and eicosapentaenoic acid in 3T3-L1 cells |
title |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
spellingShingle |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 Costa, André Gustavo Vasconcelos Lipídios Síndrome metabólica Adipocinas Lipids Metabolic syndrome Adipokines CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
title_short |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
title_full |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
title_fullStr |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
title_full_unstemmed |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
title_sort |
Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1 |
author |
Costa, André Gustavo Vasconcelos |
author_facet |
Costa, André Gustavo Vasconcelos |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5406130298857736 |
dc.contributor.author.fl_str_mv |
Costa, André Gustavo Vasconcelos |
dc.contributor.advisor-co1.fl_str_mv |
Ribeiro, Sônia Machado Rocha |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701461E0 |
dc.contributor.advisor-co2.fl_str_mv |
Silva, Fabyano Fonseca e |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766260Z2 |
dc.contributor.advisor1.fl_str_mv |
Bressan, Josefina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781728Y2 |
dc.contributor.referee1.fl_str_mv |
Aliaga, Maria Jesus Moreno |
dc.contributor.referee2.fl_str_mv |
Paula, Sérgio Oliveira de |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4 |
contributor_str_mv |
Ribeiro, Sônia Machado Rocha Silva, Fabyano Fonseca e Bressan, Josefina Aliaga, Maria Jesus Moreno Paula, Sérgio Oliveira de |
dc.subject.por.fl_str_mv |
Lipídios Síndrome metabólica Adipocinas |
topic |
Lipídios Síndrome metabólica Adipocinas Lipids Metabolic syndrome Adipokines CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Lipids Metabolic syndrome Adipokines |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS |
description |
The incidence of obesity has been drastically increasing, reaching epidemic proportions worldwide, mainly in Western and industrialized countries. Scientific evidence relates obesity to a chronic inflammatory process of low intensity, due to increased pro-inflammatory cytokines, such as an abnormal production of tumor necrosis factor alpha (TNFα). Hypertrophy and hyperplasia of adipocytes have been associated with diseases related to the metabolic syndrome, in which obesity seems to be the pivot. Therefore, the study of adipogenesis is crucial in the understanding of obesity, as well as its treatment. Studies suggest that both the eicosapentaenoic acid (EPA) and medium-chain fatty acids (MCFA) are capable of modulating the incorporation of lipids during the adipogenesis and of regulating the expression of key adipogenic transcription factors to cell differentiation. This study aimed to evaluate the effect of EPA and MCFA (C8:0 and C12:0) over the adipogenesis in 3T3-L1 cells. Moreover, the effect of such fatty acids over the mRNA expression and chemerin secretion, a new adipocyte involved in adipogenesis, was evaluated. Hence, preadipocytes were cultured in the presence (induced adipogenesis) or absence (spontaneous adipogenesis) of a hormonal cocktail. Throughout the differentiation process, cells were treated with C8:0 (250 μM), C12:0 (250 μM) and EPA (100 or 200 μM), supplemented or not with TNFα. During the experimental period, samples were collected in order to assess the gene expression of: preadipogenenic factor (DLK1), wingless related MMTV integration site 10b (WNT10b); CCAAT / enhancer-binding proteins (C/EBP) β, δ, and α; peroxisome proliferator-activated receptor gamma (PPARγ) and chemerin. The incorporation of lipids was determined by Oil Red-O. Furthermore, still regarding the study of chemerin, mature adipocytes were incubated for 24 hours with the previously described fatty acids, along with TNFα and insulin, in the presence or absence of protein kinases inhibitors. At the end of this period, an analysis of the mRNA expression and chemerin secretion was conducted. The results showed that during the induced adipogenesis, the fatty acids did not significantly alter the incorporation of lipids. On the other hand, during the spontaneous adipogenesis, EPA (200 μM) promoted a greater incorporation of lipids (p<0.05), while the tested MCFA demonstrated no such effect. When added of TNFα, the C8:0 decreased the incorporation of lipids during the induced adipogenesis, thus enhancing the inhibitory effect of this cytokine. When added of TNFα, EPA stimulated the mRNA expression of the DLK1, WNT10b and C/EBPβ, whereas the C/EBPα and PPARγ expression were inhibited. The EPA did not present significant effects over the C/EBPδ. The MCFA were able to regulate the expression of the classical transcriptional adipogenic factors (C/EPBβ, α and δ and PPARγ), with particular emphasis given to the effects achieved by the C8:0. Regarding the expression of chemerin, it was observed that, apparently, the studied fatty acids did not participate in the secretion and expression of such adipokine. However, in a 24-hour incubation study, TNFα stimulated the expression of the mRNA chemerin, as well as its secretion, possibly via phosphatidil inositol 3-kinase (PI3K). In conclusion, the results showed that the analyzed fatty acids are capable of modulating the incorporation of lipids, as well as the expression of key adipogenic transcription factors, thus potentializing the effect of the TNFα. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-06-21 |
dc.date.available.fl_str_mv |
2012-04-02 2015-03-26T12:25:04Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:25:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, André Gustavo Vasconcelos. Regulation of adipogenesis and chemerin secretion by medium-chain fatty acids and eicosapentaenoic acid in 3T3-L1 cells. 2010. 118 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2010. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/447 |
identifier_str_mv |
COSTA, André Gustavo Vasconcelos. Regulation of adipogenesis and chemerin secretion by medium-chain fatty acids and eicosapentaenoic acid in 3T3-L1 cells. 2010. 118 f. Tese (Doutorado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2010. |
url |
http://locus.ufv.br/handle/123456789/447 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Doutorado em Ciência e Tecnologia de Alimentos |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/447/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/447/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/447/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
b17d37b14c0a089284fa76e6f70a912a ec14cc91882d444a9c85bf290e89267b bdf6a30bade8fb621319c82219b1f4ae |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212848646389760 |