Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.plaphy.2018.05.028 http://www.locus.ufv.br/handle/123456789/22492 |
Resumo: | The success of Sclerotinia sclerotiorum infection relies mainly on the production of the non-host selective toxin named oxalic acid (OA). This toxin is known to play multiple roles in a host infected by the fungus, but its effect on photosynthesis and the antioxidant system of common bean plants remain elusive. Therefore, we performed detailed analysis of leaf gas exchange, chlorophyll a fluorescence, activities of antioxidant enzymes, concentrations of reactive oxygen species and photosynthetic pigments to investigate the OA's role during the S. sclerotiorum pathogenesis. To achieve this goal, common bean plants were sprayed with water or with oxalic acid (referred to as –OA and +OA plants, respectively) and either non-challenged or challenged with a wild-type (WT) or an OA-defective mutant (A4) of S. sclerotiorum. Irrespective of OA spray, the WT isolate was more aggressive than the A4 isolate and spraying OA increased OA concentration in the leaflets as well as the aggressiveness of both isolates. Biochemical limitations were behind S. sclerotiorum-induced photosynthetic impairments notably for the +OA plants inoculated with the WT isolate. Inoculated plants were not able to fully capture and exploit the collected energy due to the degradation of photosynthetic pigments. Photoinhibition of photosynthesis and photochemical dysfunctions were potentiated by OA. Higher activities of superoxide dismutase, peroxidase and ascorbate peroxidase besides reductions on catalase activity were noticed for plants inoculated with the WT isolate. OA was able to counteract most of the increases in the activities of antioxidant enzymes thereby increasing the generation of superoxide and hydrogen peroxide and the concurrent damage to the membranes of host cells as evidenced by the high malondialdehyde concentration. In conclusion, OA was found to enhance biochemical limitations to photosynthesis, photochemical dysfunctions and oxidative stress in the leaflets of common bean plants infected by S. sclerotiorum. |
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Fagundes-Nacarath, I. R. F.Debona, D.Rodrigues, F. A.2018-11-07T16:54:30Z2018-11-07T16:54:30Z2018-0809819428https://doi.org/10.1016/j.plaphy.2018.05.028http://www.locus.ufv.br/handle/123456789/22492The success of Sclerotinia sclerotiorum infection relies mainly on the production of the non-host selective toxin named oxalic acid (OA). This toxin is known to play multiple roles in a host infected by the fungus, but its effect on photosynthesis and the antioxidant system of common bean plants remain elusive. Therefore, we performed detailed analysis of leaf gas exchange, chlorophyll a fluorescence, activities of antioxidant enzymes, concentrations of reactive oxygen species and photosynthetic pigments to investigate the OA's role during the S. sclerotiorum pathogenesis. To achieve this goal, common bean plants were sprayed with water or with oxalic acid (referred to as –OA and +OA plants, respectively) and either non-challenged or challenged with a wild-type (WT) or an OA-defective mutant (A4) of S. sclerotiorum. Irrespective of OA spray, the WT isolate was more aggressive than the A4 isolate and spraying OA increased OA concentration in the leaflets as well as the aggressiveness of both isolates. Biochemical limitations were behind S. sclerotiorum-induced photosynthetic impairments notably for the +OA plants inoculated with the WT isolate. Inoculated plants were not able to fully capture and exploit the collected energy due to the degradation of photosynthetic pigments. Photoinhibition of photosynthesis and photochemical dysfunctions were potentiated by OA. Higher activities of superoxide dismutase, peroxidase and ascorbate peroxidase besides reductions on catalase activity were noticed for plants inoculated with the WT isolate. OA was able to counteract most of the increases in the activities of antioxidant enzymes thereby increasing the generation of superoxide and hydrogen peroxide and the concurrent damage to the membranes of host cells as evidenced by the high malondialdehyde concentration. In conclusion, OA was found to enhance biochemical limitations to photosynthesis, photochemical dysfunctions and oxidative stress in the leaflets of common bean plants infected by S. sclerotiorum.engPlant Physiology and BiochemistryVolume 129, Pages 109- 121, August 20182018 Elsevier Masson SAS. All rights reserved.info:eu-repo/semantics/openAccessPhaseolus vulgarisAntioxidant systemChlorophyll a fluorescenceLeaf gas exchangePhotosynthesisWhite moldOxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1905860https://locus.ufv.br//bitstream/123456789/22492/1/artigo.pdf1d9673e2daf6c0411493f27da5389003MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22492/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/224922018-11-07 14:22:12.27oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-11-07T17:22:12LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
title |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
spellingShingle |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction Fagundes-Nacarath, I. R. F. Phaseolus vulgaris Antioxidant system Chlorophyll a fluorescence Leaf gas exchange Photosynthesis White mold |
title_short |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
title_full |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
title_fullStr |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
title_full_unstemmed |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
title_sort |
Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction |
author |
Fagundes-Nacarath, I. R. F. |
author_facet |
Fagundes-Nacarath, I. R. F. Debona, D. Rodrigues, F. A. |
author_role |
author |
author2 |
Debona, D. Rodrigues, F. A. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Fagundes-Nacarath, I. R. F. Debona, D. Rodrigues, F. A. |
dc.subject.pt-BR.fl_str_mv |
Phaseolus vulgaris Antioxidant system Chlorophyll a fluorescence Leaf gas exchange Photosynthesis White mold |
topic |
Phaseolus vulgaris Antioxidant system Chlorophyll a fluorescence Leaf gas exchange Photosynthesis White mold |
description |
The success of Sclerotinia sclerotiorum infection relies mainly on the production of the non-host selective toxin named oxalic acid (OA). This toxin is known to play multiple roles in a host infected by the fungus, but its effect on photosynthesis and the antioxidant system of common bean plants remain elusive. Therefore, we performed detailed analysis of leaf gas exchange, chlorophyll a fluorescence, activities of antioxidant enzymes, concentrations of reactive oxygen species and photosynthetic pigments to investigate the OA's role during the S. sclerotiorum pathogenesis. To achieve this goal, common bean plants were sprayed with water or with oxalic acid (referred to as –OA and +OA plants, respectively) and either non-challenged or challenged with a wild-type (WT) or an OA-defective mutant (A4) of S. sclerotiorum. Irrespective of OA spray, the WT isolate was more aggressive than the A4 isolate and spraying OA increased OA concentration in the leaflets as well as the aggressiveness of both isolates. Biochemical limitations were behind S. sclerotiorum-induced photosynthetic impairments notably for the +OA plants inoculated with the WT isolate. Inoculated plants were not able to fully capture and exploit the collected energy due to the degradation of photosynthetic pigments. Photoinhibition of photosynthesis and photochemical dysfunctions were potentiated by OA. Higher activities of superoxide dismutase, peroxidase and ascorbate peroxidase besides reductions on catalase activity were noticed for plants inoculated with the WT isolate. OA was able to counteract most of the increases in the activities of antioxidant enzymes thereby increasing the generation of superoxide and hydrogen peroxide and the concurrent damage to the membranes of host cells as evidenced by the high malondialdehyde concentration. In conclusion, OA was found to enhance biochemical limitations to photosynthesis, photochemical dysfunctions and oxidative stress in the leaflets of common bean plants infected by S. sclerotiorum. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-07T16:54:30Z |
dc.date.available.fl_str_mv |
2018-11-07T16:54:30Z |
dc.date.issued.fl_str_mv |
2018-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.plaphy.2018.05.028 http://www.locus.ufv.br/handle/123456789/22492 |
dc.identifier.issn.none.fl_str_mv |
09819428 |
identifier_str_mv |
09819428 |
url |
https://doi.org/10.1016/j.plaphy.2018.05.028 http://www.locus.ufv.br/handle/123456789/22492 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 129, Pages 109- 121, August 2018 |
dc.rights.driver.fl_str_mv |
2018 Elsevier Masson SAS. All rights reserved. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
2018 Elsevier Masson SAS. All rights reserved. |
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openAccess |
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Plant Physiology and Biochemistry |
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Plant Physiology and Biochemistry |
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