Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x http://www.locus.ufv.br/handle/123456789/22948 |
Resumo: | The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae. |
id |
UFV_c6159d791607540139cf2f6affc7bc61 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/22948 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Lima, M. A. P.Pires, C. S. S.Guedes, R. N. C.Nakasu, E. Y. T.Lara, M. S.Fontes, E. M. G.Sujii, E. R.Dias, S. C.Campos, L. A. O.2019-01-09T11:23:37Z2019-01-09T11:23:37Z2011-071439-0418http://dx.doi.org/10.1111/j.1439-0418.2010.01573.xhttp://www.locus.ufv.br/handle/123456789/22948The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae.engJournal of Applied EntomologyVolume 135, Issue 6, Pages 415- 422, July 2011Bt cottonGMOPollinator larvaeRisk assessmentTransgenic plantsDoes Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf303654https://locus.ufv.br//bitstream/123456789/22948/1/artigo.pdfe21f4eabacfa4abe20d11036a0ac20edMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22948/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/229482019-01-09 09:49:13.135oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-01-09T12:49:13LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
title |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
spellingShingle |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? Lima, M. A. P. Bt cotton GMO Pollinator larvae Risk assessment Transgenic plants |
title_short |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
title_full |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
title_fullStr |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
title_full_unstemmed |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
title_sort |
Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee? |
author |
Lima, M. A. P. |
author_facet |
Lima, M. A. P. Pires, C. S. S. Guedes, R. N. C. Nakasu, E. Y. T. Lara, M. S. Fontes, E. M. G. Sujii, E. R. Dias, S. C. Campos, L. A. O. |
author_role |
author |
author2 |
Pires, C. S. S. Guedes, R. N. C. Nakasu, E. Y. T. Lara, M. S. Fontes, E. M. G. Sujii, E. R. Dias, S. C. Campos, L. A. O. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, M. A. P. Pires, C. S. S. Guedes, R. N. C. Nakasu, E. Y. T. Lara, M. S. Fontes, E. M. G. Sujii, E. R. Dias, S. C. Campos, L. A. O. |
dc.subject.pt-BR.fl_str_mv |
Bt cotton GMO Pollinator larvae Risk assessment Transgenic plants |
topic |
Bt cotton GMO Pollinator larvae Risk assessment Transgenic plants |
description |
The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-07 |
dc.date.accessioned.fl_str_mv |
2019-01-09T11:23:37Z |
dc.date.available.fl_str_mv |
2019-01-09T11:23:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x http://www.locus.ufv.br/handle/123456789/22948 |
dc.identifier.issn.none.fl_str_mv |
1439-0418 |
identifier_str_mv |
1439-0418 |
url |
http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x http://www.locus.ufv.br/handle/123456789/22948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 135, Issue 6, Pages 415- 422, July 2011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Applied Entomology |
publisher.none.fl_str_mv |
Journal of Applied Entomology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/22948/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/22948/2/license.txt |
bitstream.checksum.fl_str_mv |
e21f4eabacfa4abe20d11036a0ac20ed 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212961725874176 |