Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?

Detalhes bibliográficos
Autor(a) principal: Lima, M. A. P.
Data de Publicação: 2011
Outros Autores: Pires, C. S. S., Guedes, R. N. C., Nakasu, E. Y. T., Lara, M. S., Fontes, E. M. G., Sujii, E. R., Dias, S. C., Campos, L. A. O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x
http://www.locus.ufv.br/handle/123456789/22948
Resumo: The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae.
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spelling Lima, M. A. P.Pires, C. S. S.Guedes, R. N. C.Nakasu, E. Y. T.Lara, M. S.Fontes, E. M. G.Sujii, E. R.Dias, S. C.Campos, L. A. O.2019-01-09T11:23:37Z2019-01-09T11:23:37Z2011-071439-0418http://dx.doi.org/10.1111/j.1439-0418.2010.01573.xhttp://www.locus.ufv.br/handle/123456789/22948The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae.engJournal of Applied EntomologyVolume 135, Issue 6, Pages 415- 422, July 2011Bt cottonGMOPollinator larvaeRisk assessmentTransgenic plantsDoes Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf303654https://locus.ufv.br//bitstream/123456789/22948/1/artigo.pdfe21f4eabacfa4abe20d11036a0ac20edMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22948/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/229482019-01-09 09:49:13.135oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-01-09T12:49:13LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
title Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
spellingShingle Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
Lima, M. A. P.
Bt cotton
GMO
Pollinator larvae
Risk assessment
Transgenic plants
title_short Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
title_full Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
title_fullStr Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
title_full_unstemmed Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
title_sort Does Cry1Ac Bt-toxin impair development of worker larvae of Africanized honey bee?
author Lima, M. A. P.
author_facet Lima, M. A. P.
Pires, C. S. S.
Guedes, R. N. C.
Nakasu, E. Y. T.
Lara, M. S.
Fontes, E. M. G.
Sujii, E. R.
Dias, S. C.
Campos, L. A. O.
author_role author
author2 Pires, C. S. S.
Guedes, R. N. C.
Nakasu, E. Y. T.
Lara, M. S.
Fontes, E. M. G.
Sujii, E. R.
Dias, S. C.
Campos, L. A. O.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, M. A. P.
Pires, C. S. S.
Guedes, R. N. C.
Nakasu, E. Y. T.
Lara, M. S.
Fontes, E. M. G.
Sujii, E. R.
Dias, S. C.
Campos, L. A. O.
dc.subject.pt-BR.fl_str_mv Bt cotton
GMO
Pollinator larvae
Risk assessment
Transgenic plants
topic Bt cotton
GMO
Pollinator larvae
Risk assessment
Transgenic plants
description The western honey bee (Apis mellifera L.) is a widespread pollinator species. The present study aimed to test if Africanized honey bee larvae are negatively affected by the ingestion of diet contaminated with the Bacillus thunringiensis toxin Cry1Ac, which is expressed in GM cotton plants. The toxin activity was confirmed in bioassays with the velvetbean caterpillar (Anticarsia gemmatalis), a soybean pest species susceptible to Cry1Ac. The honey bee larvae were subjected to ingestion of either pure larval diet (control), diluted larval diet (diluted control) or larval diet diluted in a Cry1Ac solution at a concentration compatible with the maximum possible field exposure. Although diluted diet slightly increased larval mortality, Cry1Ac ingestion did not affect survival, developmental time, and neither adult body mass nor size, indicating that GM plants are unlikely to significantly impair the development of honey bee larvae. The larval-rearing system reported here was suitable to assess the lethal and sub-lethal effects of GM expressed toxins against honey bee larvae.
publishDate 2011
dc.date.issued.fl_str_mv 2011-07
dc.date.accessioned.fl_str_mv 2019-01-09T11:23:37Z
dc.date.available.fl_str_mv 2019-01-09T11:23:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x
http://www.locus.ufv.br/handle/123456789/22948
dc.identifier.issn.none.fl_str_mv 1439-0418
identifier_str_mv 1439-0418
url http://dx.doi.org/10.1111/j.1439-0418.2010.01573.x
http://www.locus.ufv.br/handle/123456789/22948
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 135, Issue 6, Pages 415- 422, July 2011
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dc.publisher.none.fl_str_mv Journal of Applied Entomology
publisher.none.fl_str_mv Journal of Applied Entomology
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