Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1111/jam.13279 http://www.locus.ufv.br/handle/123456789/15010 |
Resumo: | To investigate the anti-HSV and anti-inflammatory effects of a standardized ethyl acetate extract (SEAE) prepared with the stem bark of Strychnos pseudoquina, along with two isolated compounds: quercetin 3-O-methyl ether (3MQ) and strychnobiflavone (SBF). The mechanisms of action were evaluated by different methodological strategies. SEAE and SBF affected the early stages of viral infection and reduced HSV-1 protein expression. Both flavonoids elicited a concentration-dependent inhibition of monocyte chemoattractant protein-1 (MCP-1), whereas 3MQ reduced the chemokine release more significantly than SBF. Conversely, both compounds stimulated the production of the cytokines TNF-α and IL-1-β in LPS-stimulated cells, especially at the intermediate and the highest tested concentrations. SEAE and SBF interfered with various steps of HSV replication cycle, mainly adsorption, postadsorption and penetration, as well as with β and γ viral proteins expression; moreover, a direct inactivation of viral particles was observed. Besides, both flavonoids inhibited MCP-1 selectively, a feature that may be beneficial for the development of new anti-HSV agents. The results indicated that the samples present anti-HSV and anti-inflammatory activities, at different levels, which is an interesting feature since cold and genital sores are accompanied by an inflammation process. |
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Boff, L.Silva, I.T.Argenta, D.F.Farias, L.M.Alvarenga, L.F.Pádua, R.M.Braga, F.C.Leite, J.P.V.Kratz, J.M.Simões, C.M.O.2017-12-14T10:07:53Z2017-12-14T10:07:53Z2016-08-221364-5072http://dx.doi.org/10.1111/jam.13279http://www.locus.ufv.br/handle/123456789/15010To investigate the anti-HSV and anti-inflammatory effects of a standardized ethyl acetate extract (SEAE) prepared with the stem bark of Strychnos pseudoquina, along with two isolated compounds: quercetin 3-O-methyl ether (3MQ) and strychnobiflavone (SBF). The mechanisms of action were evaluated by different methodological strategies. SEAE and SBF affected the early stages of viral infection and reduced HSV-1 protein expression. Both flavonoids elicited a concentration-dependent inhibition of monocyte chemoattractant protein-1 (MCP-1), whereas 3MQ reduced the chemokine release more significantly than SBF. Conversely, both compounds stimulated the production of the cytokines TNF-α and IL-1-β in LPS-stimulated cells, especially at the intermediate and the highest tested concentrations. SEAE and SBF interfered with various steps of HSV replication cycle, mainly adsorption, postadsorption and penetration, as well as with β and γ viral proteins expression; moreover, a direct inactivation of viral particles was observed. Besides, both flavonoids inhibited MCP-1 selectively, a feature that may be beneficial for the development of new anti-HSV agents. The results indicated that the samples present anti-HSV and anti-inflammatory activities, at different levels, which is an interesting feature since cold and genital sores are accompanied by an inflammation process.engJournal of Applied Microbiology121(6), p. 1519-1529, Dec. 2016Strychnos pseudoquinaHSV-1HSV-2Quercetin 3-O-methyl etherStandardized extractStrychnobiflavoneStrychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALBoff_et_al-2016-Journal_of_Applied_Microbiology.pdfBoff_et_al-2016-Journal_of_Applied_Microbiology.pdftexto completoapplication/pdf375916https://locus.ufv.br//bitstream/123456789/15010/1/Boff_et_al-2016-Journal_of_Applied_Microbiology.pdf92c0e267bd7511dc35f36f3f08e395c3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/15010/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILBoff_et_al-2016-Journal_of_Applied_Microbiology.pdf.jpgBoff_et_al-2016-Journal_of_Applied_Microbiology.pdf.jpgIM Thumbnailimage/jpeg5792https://locus.ufv.br//bitstream/123456789/15010/3/Boff_et_al-2016-Journal_of_Applied_Microbiology.pdf.jpg59fab6c0a2f554dde0dd67367dfc05f1MD53123456789/150102017-12-14 22:00:56.248oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-12-15T01:00:56LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
title |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
spellingShingle |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity Boff, L. Strychnos pseudoquina HSV-1 HSV-2 Quercetin 3-O-methyl ether Standardized extract Strychnobiflavone |
title_short |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
title_full |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
title_fullStr |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
title_full_unstemmed |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
title_sort |
Strychnos pseudoquina A. St. Hil.: a brazilian medicinal plant with promising in vitro antiherpes activity |
author |
Boff, L. |
author_facet |
Boff, L. Silva, I.T. Argenta, D.F. Farias, L.M. Alvarenga, L.F. Pádua, R.M. Braga, F.C. Leite, J.P.V. Kratz, J.M. Simões, C.M.O. |
author_role |
author |
author2 |
Silva, I.T. Argenta, D.F. Farias, L.M. Alvarenga, L.F. Pádua, R.M. Braga, F.C. Leite, J.P.V. Kratz, J.M. Simões, C.M.O. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Boff, L. Silva, I.T. Argenta, D.F. Farias, L.M. Alvarenga, L.F. Pádua, R.M. Braga, F.C. Leite, J.P.V. Kratz, J.M. Simões, C.M.O. |
dc.subject.pt-BR.fl_str_mv |
Strychnos pseudoquina HSV-1 HSV-2 Quercetin 3-O-methyl ether Standardized extract Strychnobiflavone |
topic |
Strychnos pseudoquina HSV-1 HSV-2 Quercetin 3-O-methyl ether Standardized extract Strychnobiflavone |
description |
To investigate the anti-HSV and anti-inflammatory effects of a standardized ethyl acetate extract (SEAE) prepared with the stem bark of Strychnos pseudoquina, along with two isolated compounds: quercetin 3-O-methyl ether (3MQ) and strychnobiflavone (SBF). The mechanisms of action were evaluated by different methodological strategies. SEAE and SBF affected the early stages of viral infection and reduced HSV-1 protein expression. Both flavonoids elicited a concentration-dependent inhibition of monocyte chemoattractant protein-1 (MCP-1), whereas 3MQ reduced the chemokine release more significantly than SBF. Conversely, both compounds stimulated the production of the cytokines TNF-α and IL-1-β in LPS-stimulated cells, especially at the intermediate and the highest tested concentrations. SEAE and SBF interfered with various steps of HSV replication cycle, mainly adsorption, postadsorption and penetration, as well as with β and γ viral proteins expression; moreover, a direct inactivation of viral particles was observed. Besides, both flavonoids inhibited MCP-1 selectively, a feature that may be beneficial for the development of new anti-HSV agents. The results indicated that the samples present anti-HSV and anti-inflammatory activities, at different levels, which is an interesting feature since cold and genital sores are accompanied by an inflammation process. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-08-22 |
dc.date.accessioned.fl_str_mv |
2017-12-14T10:07:53Z |
dc.date.available.fl_str_mv |
2017-12-14T10:07:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jam.13279 http://www.locus.ufv.br/handle/123456789/15010 |
dc.identifier.issn.none.fl_str_mv |
1364-5072 |
identifier_str_mv |
1364-5072 |
url |
http://dx.doi.org/10.1111/jam.13279 http://www.locus.ufv.br/handle/123456789/15010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
121(6), p. 1519-1529, Dec. 2016 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Journal of Applied Microbiology |
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Journal of Applied Microbiology |
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