Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content

Detalhes bibliográficos
Autor(a) principal: Carvalho, Bruno Pena
Data de Publicação: 2019
Outros Autores: Costa, Fernanda de Queirós, Detoni, Danielly, Rosa, Felipe Borges, Dias, Angelo José Burla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
DOI: 10.1590/rbz4820180322
Texto Completo: https://locus.ufv.br//handle/123456789/30816
https://doi.org/10.1590/rbz4820180322
Resumo: This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period
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spelling Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid contentcryotolerancepolyunsaturated fatty acidvitrificationThis study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation periodBrazilian Journal of Animal Science2023-05-05T17:37:00Z2023-05-05T17:37:00Z2019-09-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCarvalho, B. P.; Costa, F. Q.; Detoni, D.; Rosa, F. B. and Dias, A. J. B. 2019. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content. Revista Brasileira de Zootecnia 48:e201803221806-9290https://locus.ufv.br//handle/123456789/30816https://doi.org/10.1590/rbz4820180322engR. Bras. Zootec., 48:e20180322, 2019Creative Commons Attribution Licenseinfo:eu-repo/semantics/openAccessCarvalho, Bruno PenaCosta, Fernanda de QueirósDetoni, DaniellyRosa, Felipe BorgesDias, Angelo José Burlareponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:25:34Zoai:locus.ufv.br:123456789/30816Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:25:34LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
title Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
spellingShingle Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
Carvalho, Bruno Pena
cryotolerance
polyunsaturated fatty acid
vitrification
Carvalho, Bruno Pena
cryotolerance
polyunsaturated fatty acid
vitrification
title_short Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
title_full Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
title_fullStr Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
title_full_unstemmed Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
title_sort Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
author Carvalho, Bruno Pena
author_facet Carvalho, Bruno Pena
Carvalho, Bruno Pena
Costa, Fernanda de Queirós
Detoni, Danielly
Rosa, Felipe Borges
Dias, Angelo José Burla
Costa, Fernanda de Queirós
Detoni, Danielly
Rosa, Felipe Borges
Dias, Angelo José Burla
author_role author
author2 Costa, Fernanda de Queirós
Detoni, Danielly
Rosa, Felipe Borges
Dias, Angelo José Burla
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Bruno Pena
Costa, Fernanda de Queirós
Detoni, Danielly
Rosa, Felipe Borges
Dias, Angelo José Burla
dc.subject.por.fl_str_mv cryotolerance
polyunsaturated fatty acid
vitrification
topic cryotolerance
polyunsaturated fatty acid
vitrification
description This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period
publishDate 2019
dc.date.none.fl_str_mv 2019-09-23
2023-05-05T17:37:00Z
2023-05-05T17:37:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Carvalho, B. P.; Costa, F. Q.; Detoni, D.; Rosa, F. B. and Dias, A. J. B. 2019. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content. Revista Brasileira de Zootecnia 48:e20180322
1806-9290
https://locus.ufv.br//handle/123456789/30816
https://doi.org/10.1590/rbz4820180322
identifier_str_mv Carvalho, B. P.; Costa, F. Q.; Detoni, D.; Rosa, F. B. and Dias, A. J. B. 2019. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content. Revista Brasileira de Zootecnia 48:e20180322
1806-9290
url https://locus.ufv.br//handle/123456789/30816
https://doi.org/10.1590/rbz4820180322
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv R. Bras. Zootec., 48:e20180322, 2019
dc.rights.driver.fl_str_mv Creative Commons Attribution License
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Creative Commons Attribution License
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journal of Animal Science
publisher.none.fl_str_mv Brazilian Journal of Animal Science
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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dc.identifier.doi.none.fl_str_mv 10.1590/rbz4820180322