Indução à espermiação e características seminais do anura inativo rã-manteiga (Leptodactylus ocellatus) e do exótico rã-touro (Lithobates catesbeianus)

• Autor(a) principal: Pereira, Marcelo Maia
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2238
• topic: Indução; Sêmen; Rã-manteiga; Rã-touro; Acetato de buserelina; Induction; Semen; Butterfrog; Bullfrog; Buserelin acetate; CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA

In recent years several facts are leading to extinction and decline of amphibian populations. Captive breeding and knowledge of reproduction may be strategies for the early signs disappeared. The use of hormone for inducing spermiation is necessary to obtain semen quality that will be used for artificial fertilization, cryopreservation, animal breeding and subsequent repopulation of species. There were two papers: the first was carried out to verify the use of buserelin acetate as an inducer of the spermiation butterfrog and analyze semen butterfrog and analyzed the semen of bullfrog. The location of the works was the Experimental Frogfarm Federal University of Viçosa, the period of two of three from February to May 2009. Experiment 1: We conducted the experiment in order to induce spermiation using buserelin acetate and analyzing the semen collected. The job site was the Experimental Frog Federal University of Viçosa, in the period of two of three from February to May 2009. We used seven male butterfrog who had secondary sexual characteristics: black spines, limbs and developed to reflect the presence of embrace. The hormone was used buserelin acetate (GnRHa) at a dose of 0.4 g and was applied for seven days at intervals of 24 hours, 90 minutes after the application was performed daily collection of semen parameters used for semen evaluation were: semen volume, color of semen, sperm motility, sperm motility, sperm concentration and morphology assessment of spermatozoa. Only three animals responded and in total were collected seven samples of semen, the first collection took 97.5 hours after the first application. The semen butterfrog obtained on average volume of 0.38 mL, color vision, motility of 3.71, sperm motility of 77.14%, concentration of 6.60 x106 SPTZ / mL, 69% of normal spermatozoa. Buserelin acetate can be used to induce spermiation in the frogbutter. We collected low semen volume, the results of color, vigor, motility, concentration and normal sperm counts are acceptable. Experiment 2: We conducted the experiment in order to induce spermiation of males using buserelin acetate and analyzing the semen collected. The job site was the Experimental Frog Federal University of Viçosa, in the period of two of three from February to May 2009. We used five male bullfrogs that had secondary sexual characteristics: weight greater than 200g, with yellow chat, presence of callus and Bridal person's reflection to embrace. The hormone was used buserelin acetate (GnRHa) at a dose of 0.4 g, 60 minutes after single application was made to collect the semen parameters used for semen evaluation were: semen volume, color of semen, sperm motility, sperm motility, sperm concentration and morphology assessment of spermatozoa. The semen of bullfrog obtained on average volume of 5.76 mL, color vision, motility of 4.80, motility of 93%, and concentration of 14.24 x106 SPTZ / mL, 70% of normal spermatozoa. The results found in semen samples of bullfrog volume, color, vigor, motility, sperm concentration and normal sperm counts are acceptable. All the parameters used for evaluation of semen samples from bullfrog are essential for optimal completion of the semen quality and decision making to their destination.