Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.snb.2013.06.022 http://www.locus.ufv.br/handle/123456789/23683 |
Resumo: | The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus,Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in PCDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses.The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus, Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in CDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses. |
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Oliveira, Taíla V. deSoares, Nilda de F. F.Silva, Deusanilde J.Andrade, Nélio J. deMedeiros, Eber Antônio A.Badaró, Amanda T.2019-02-22T14:39:45Z2019-02-22T14:39:45Z2013-110925-4005https://doi.org/10.1016/j.snb.2013.06.022http://www.locus.ufv.br/handle/123456789/23683The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus,Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in PCDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses.The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus, Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in CDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses.engSensors and Actuators B: ChemicalVolume 188, Pages 385- 392, November 20132013 Published by Elsevier B.V.info:eu-repo/semantics/openAccessPolydiacetylene vesicleLysineDetection of S. CholeraesuisDevelopment of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1424093https://locus.ufv.br//bitstream/123456789/23683/1/artigo.pdfacf88ac7b8f0f1cf6c5939b9a127181fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23683/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/236832019-02-22 11:51:41.44oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-02-22T14:51:41LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
title |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
spellingShingle |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria Oliveira, Taíla V. de Polydiacetylene vesicle Lysine Detection of S. Choleraesuis |
title_short |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
title_full |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
title_fullStr |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
title_full_unstemmed |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
title_sort |
Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria |
author |
Oliveira, Taíla V. de |
author_facet |
Oliveira, Taíla V. de Soares, Nilda de F. F. Silva, Deusanilde J. Andrade, Nélio J. de Medeiros, Eber Antônio A. Badaró, Amanda T. |
author_role |
author |
author2 |
Soares, Nilda de F. F. Silva, Deusanilde J. Andrade, Nélio J. de Medeiros, Eber Antônio A. Badaró, Amanda T. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Taíla V. de Soares, Nilda de F. F. Silva, Deusanilde J. Andrade, Nélio J. de Medeiros, Eber Antônio A. Badaró, Amanda T. |
dc.subject.pt-BR.fl_str_mv |
Polydiacetylene vesicle Lysine Detection of S. Choleraesuis |
topic |
Polydiacetylene vesicle Lysine Detection of S. Choleraesuis |
description |
The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus,Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in PCDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses.The behavior of blue polydiacetylene vesicles was studied in the presence of pathogens to evaluate their application as sensors for the food industry. In this study, lysine-containing vesicles of 10,12-pentacosadyinoic acid (PCDA)/dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and PCDA/Sphingomyelin (SPH)/Cholesterol (CHO) were tested to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus, Salmonella Choleraesuis and Escherichia coli). The structures were characterized using the dynamic light scattering technique in which the intensity correlation functions of dilute solutions were fitted using cumulant analysis. The PCDA/DMPC vesicles exhibited improved molecular packing compared to the PCDA/SPH/CHO vesicles, with a particle size of approximately 150 nm for the PCDA/DMPC vesicles compared to 200 nm for the PCDA/SPH/CHO vesicles. Subsequent to the vesicle assembly, their colorimetric response was evaluated. Importantly, the colorimetric response was more pronounced for the PCDA/SPH/CHO/Lysine vesicles than for the PCDA/DMPC/Lysine vesicles, indicating that the SPH/CHO decreased the activation barrier, thus improving the bacterial colorimetric response. The PCDA concentration influenced the vesicle selectivity, with 1 mM PCDA exhibiting a slightly greater sensitivity to S.Choleraesuis than the other bacteria, while 2 mM and 3 mM PCDA exhibited greater selectivity for E. coli and S. aureus. Likewise, 1 mM PCDA and the phospholipids SPH/CHO favored the detection of S. Choleraesuis, and due to the complexity of the bacteria behavior, additional studies were conducted. The colorimetric response was evaluated at various lysine concentrations (6.7 μg ml−1; 35.0 μg ml−1 and 63.0 μg ml−1) and pH values (5.0, 6.0 and 7.0) to improve the PCDA/SPH/CHO/Lysine vesicle specificity. At a lysine concentration of 6.70 μg ml−1, no color change in the PCDA/SPH/CHO/Lysine vesicles was observed in the absence of S. Choleraesuis, indicating that they were suitable for use in the food industry. In terms of pH, the expression of the lysine-dependent system was verified in CDA/SPH/CO/Lysine vesicles at pH 5.0, although an increase in the pH value to approximately 6.5 provided the highest colorimetric response. Therefore, the PCDA/SPH/CHO/Lysine vesicles demonstrated great potential for use as a biosensor to detect food pathogens in routine analyses. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-11 |
dc.date.accessioned.fl_str_mv |
2019-02-22T14:39:45Z |
dc.date.available.fl_str_mv |
2019-02-22T14:39:45Z |
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info:eu-repo/semantics/publishedVersion |
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dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.snb.2013.06.022 http://www.locus.ufv.br/handle/123456789/23683 |
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0925-4005 |
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0925-4005 |
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https://doi.org/10.1016/j.snb.2013.06.022 http://www.locus.ufv.br/handle/123456789/23683 |
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eng |
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Volume 188, Pages 385- 392, November 2013 |
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2013 Published by Elsevier B.V. |
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