NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1038/nature14171 http://www.locus.ufv.br/handle/123456789/19979 |
Resumo: | Plants and plant pathogens are subject to continuous co-evolutionary pressure for dominance, and the outcomes of these interactions can substantially impact agriculture and food security^ 1–3 . In virus– plant interactions, one of the major mechanisms for plant antiviral immunity relies on RNA silencing, which is often suppressed by co-evolving virus suppressors, thus enhancing viral pathogenicity in susceptible hosts^ 1 . In addition, plants use the nucleotide-binding and leucine-rich repeat (NB-LRR) domain-containing resistance proteins, which recognize viral effectors to activate effector-triggered immunity in a defence mechanism similar to that employed in non-viral infections^ 2,3 . Unlike most eukaryotic organisms, plants are not known to activate mechanisms of host global translation suppression to fight viruses^ 1,2 . Here we demonstrate in Arabidopsis that the constitutive activation of NIK1, a leucine-rich repeat receptor-like kinase (LRR-RLK) identified as a virulence target of the begomovirus nuclear shuttle protein (NSP)^ 4–6 , leads to global translation suppression and translocation of the downstream component RPL10 to the nucleus, where it interacts with a newly identified MYB-like protein, L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN (LIMYB), to downregulate translational machinery genes fully. LIMYB overexpression represses ribosomal protein genes at the transcriptional level, resulting in protein synthesis inhibition, decreased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus. By contrast, the loss of LIMYB function releases the repression of translation-related genes and increases susceptibility to virus infection. Therefore, LIMYB links immune receptor LRR-RLK activation to global translation suppression as an antiviral immunity strategy in plants. |
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Zorzatto, CristianeMachado, João Paulo B.Lopes, Kênia V. G.Nascimento, Kelly J. T.Pereira, Welison A.Brustolini, Otávio J. B.Reis, Pedro A. B.Calil, Iara P.Deguchi, MichihitoSachetto-Martins, GilbertoGouveia, Bianca C.Loriato, Virgílio A. P.Silva, Marcos A. C.Silva, Fabyano F.Santos, Anésia A.Chory, JoanneFontes, Elizabeth P. B.2018-06-06T15:40:03Z2018-06-06T15:40:03Z2015-04-3014764687http://dx.doi.org/10.1038/nature14171http://www.locus.ufv.br/handle/123456789/19979Plants and plant pathogens are subject to continuous co-evolutionary pressure for dominance, and the outcomes of these interactions can substantially impact agriculture and food security^ 1–3 . In virus– plant interactions, one of the major mechanisms for plant antiviral immunity relies on RNA silencing, which is often suppressed by co-evolving virus suppressors, thus enhancing viral pathogenicity in susceptible hosts^ 1 . In addition, plants use the nucleotide-binding and leucine-rich repeat (NB-LRR) domain-containing resistance proteins, which recognize viral effectors to activate effector-triggered immunity in a defence mechanism similar to that employed in non-viral infections^ 2,3 . Unlike most eukaryotic organisms, plants are not known to activate mechanisms of host global translation suppression to fight viruses^ 1,2 . Here we demonstrate in Arabidopsis that the constitutive activation of NIK1, a leucine-rich repeat receptor-like kinase (LRR-RLK) identified as a virulence target of the begomovirus nuclear shuttle protein (NSP)^ 4–6 , leads to global translation suppression and translocation of the downstream component RPL10 to the nucleus, where it interacts with a newly identified MYB-like protein, L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN (LIMYB), to downregulate translational machinery genes fully. LIMYB overexpression represses ribosomal protein genes at the transcriptional level, resulting in protein synthesis inhibition, decreased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus. By contrast, the loss of LIMYB function releases the repression of translation-related genes and increases susceptibility to virus infection. Therefore, LIMYB links immune receptor LRR-RLK activation to global translation suppression as an antiviral immunity strategy in plants.engNaturev. 520, n. 7549, p. 679–682, Abril 2015Macmillan Publishers Limited, part of Springer Natureinfo:eu-repo/semantics/openAccessNIK1Antiviral immunityPlantNIK1-mediated translation suppression functions as a plant antiviral immunity mechanisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1804611https://locus.ufv.br//bitstream/123456789/19979/1/artigo.pdfd7417abbefe703a78f30d3c540054b97MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19979/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5149https://locus.ufv.br//bitstream/123456789/19979/3/artigo.pdf.jpg235b2fdceb7e4c4823dffafa97475eacMD53123456789/199792018-06-06 23:00:38.99oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-06-07T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
title |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
spellingShingle |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism Zorzatto, Cristiane NIK1 Antiviral immunity Plant |
title_short |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
title_full |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
title_fullStr |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
title_full_unstemmed |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
title_sort |
NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism |
author |
Zorzatto, Cristiane |
author_facet |
Zorzatto, Cristiane Machado, João Paulo B. Lopes, Kênia V. G. Nascimento, Kelly J. T. Pereira, Welison A. Brustolini, Otávio J. B. Reis, Pedro A. B. Calil, Iara P. Deguchi, Michihito Sachetto-Martins, Gilberto Gouveia, Bianca C. Loriato, Virgílio A. P. Silva, Marcos A. C. Silva, Fabyano F. Santos, Anésia A. Chory, Joanne Fontes, Elizabeth P. B. |
author_role |
author |
author2 |
Machado, João Paulo B. Lopes, Kênia V. G. Nascimento, Kelly J. T. Pereira, Welison A. Brustolini, Otávio J. B. Reis, Pedro A. B. Calil, Iara P. Deguchi, Michihito Sachetto-Martins, Gilberto Gouveia, Bianca C. Loriato, Virgílio A. P. Silva, Marcos A. C. Silva, Fabyano F. Santos, Anésia A. Chory, Joanne Fontes, Elizabeth P. B. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zorzatto, Cristiane Machado, João Paulo B. Lopes, Kênia V. G. Nascimento, Kelly J. T. Pereira, Welison A. Brustolini, Otávio J. B. Reis, Pedro A. B. Calil, Iara P. Deguchi, Michihito Sachetto-Martins, Gilberto Gouveia, Bianca C. Loriato, Virgílio A. P. Silva, Marcos A. C. Silva, Fabyano F. Santos, Anésia A. Chory, Joanne Fontes, Elizabeth P. B. |
dc.subject.pt-BR.fl_str_mv |
NIK1 Antiviral immunity Plant |
topic |
NIK1 Antiviral immunity Plant |
description |
Plants and plant pathogens are subject to continuous co-evolutionary pressure for dominance, and the outcomes of these interactions can substantially impact agriculture and food security^ 1–3 . In virus– plant interactions, one of the major mechanisms for plant antiviral immunity relies on RNA silencing, which is often suppressed by co-evolving virus suppressors, thus enhancing viral pathogenicity in susceptible hosts^ 1 . In addition, plants use the nucleotide-binding and leucine-rich repeat (NB-LRR) domain-containing resistance proteins, which recognize viral effectors to activate effector-triggered immunity in a defence mechanism similar to that employed in non-viral infections^ 2,3 . Unlike most eukaryotic organisms, plants are not known to activate mechanisms of host global translation suppression to fight viruses^ 1,2 . Here we demonstrate in Arabidopsis that the constitutive activation of NIK1, a leucine-rich repeat receptor-like kinase (LRR-RLK) identified as a virulence target of the begomovirus nuclear shuttle protein (NSP)^ 4–6 , leads to global translation suppression and translocation of the downstream component RPL10 to the nucleus, where it interacts with a newly identified MYB-like protein, L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN (LIMYB), to downregulate translational machinery genes fully. LIMYB overexpression represses ribosomal protein genes at the transcriptional level, resulting in protein synthesis inhibition, decreased viral messenger RNA association with polysome fractions and enhanced tolerance to begomovirus. By contrast, the loss of LIMYB function releases the repression of translation-related genes and increases susceptibility to virus infection. Therefore, LIMYB links immune receptor LRR-RLK activation to global translation suppression as an antiviral immunity strategy in plants. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-04-30 |
dc.date.accessioned.fl_str_mv |
2018-06-06T15:40:03Z |
dc.date.available.fl_str_mv |
2018-06-06T15:40:03Z |
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info:eu-repo/semantics/publishedVersion |
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http://dx.doi.org/10.1038/nature14171 http://www.locus.ufv.br/handle/123456789/19979 |
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14764687 |
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14764687 |
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http://dx.doi.org/10.1038/nature14171 http://www.locus.ufv.br/handle/123456789/19979 |
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eng |
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v. 520, n. 7549, p. 679–682, Abril 2015 |
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