Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4735 |
Resumo: | A model of an international energy matrix, ideally based on renewable and cleaner energy regarded to petrol, is justified because of the increasing awareness on the global warming issue. Moreover, researches on biofuel, as an alternative source of energy, are essential to guarantee the current ranking of the Brazilian energy autonomy, in addition to the interest in production of surplus so to guarantee exportation and generation of foreign exchange. Cellulose is the most abundant renewable biomass in the planet, therefore a good energetic alternative to petrol. Sugarcane has 2/3 of its mass in lingocellulosic material, an abundant energetic resource which is not efficiently used by the current technologies, therefore, representing an enormous potential for the production of lingocellulosic ethanol. Cellulose can be hydrolyzed in glucose residuals by enzymatic treatment as well as base of acids. Technically, there are some barriers associated to acid and /or enzymatic hydrolysis which need to be overcome. Considering enzymatic hydrolyses, the reaction takes place in a non-aggressive medium, however it takes a long waiting time (from 60 to 72 hours) to occur and the lignin compound is one of the main obstacles for the enzyme access to the cellulose. Thus, the present work aimed at obtaining progress for researches on cellulosic ethanol from the bagasse and, potentially, from other sugarcane by-products through selection of clones that shows high potential bioconvertibility fibers. The experiment was made up of 286 clones and 2 witnesses (RB867515 and SP80-1842) in 25 blocks. They were selected from 13 families of half-siblings and they were vegetative propagation first generation clones. Accordingly, it was carried out correlations among desirable biochemical and agronomical traits, evaluating the cause and effect relationship on fibers and lignin of the stem. It was also estimated the possibility on defining characters which have to be used to carry out a recurrent selection program on sugarcane aiming at alcohol production. It was selected 10 clones with highest lignin content and 10 clones with the lowest lignin content in the bagasse. Those 20 clones were evaluated for the possibility in obtaining a higher glucose content after enzymatic hydrolysis process for cellulosic alcohol production. It could be observed that the higher the content of lignin in the bagasse, the lower the content of glucose produced at the end of hydrolyzesprocess. It was also observed that hemicellulose is more efficient on the conversion than saccharification. Hemicellulose functions as a positive factor on fermentable sugar production for fermentation, because it breaks down cellulose structure allowing access of the enzymes and because of its rupture it supplies glucose molecules for fermentation. Therefore, selected clones show a high potential use on sugar cane genetic breeding, even though it is still needed phenotypic and gene expression analysis studies, enzymatic quantification and syntheses of metabolic, correlating them to syntheses of lignin. |
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Baffa, David Carlos Ferreirahttp://lattes.cnpq.br/1624329861472308Loureiro, Marcelo Ehlershttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3Peternelli, Luiz Alexandrehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723301Z7Barbosa, Marcio Henrique Pereirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782585E6Diola, Valdirhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4133062U6Lopes, Francis Julio Fagundeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762375P52015-03-26T13:42:18Z2012-03-262015-03-26T13:42:18Z2010-07-22BAFFA, David Carlos Ferreira. Heritability and genotypic correlations of agronomic traits, cell wall constituents and saccharification in cane sugar. 2010. 65 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/4735A model of an international energy matrix, ideally based on renewable and cleaner energy regarded to petrol, is justified because of the increasing awareness on the global warming issue. Moreover, researches on biofuel, as an alternative source of energy, are essential to guarantee the current ranking of the Brazilian energy autonomy, in addition to the interest in production of surplus so to guarantee exportation and generation of foreign exchange. Cellulose is the most abundant renewable biomass in the planet, therefore a good energetic alternative to petrol. Sugarcane has 2/3 of its mass in lingocellulosic material, an abundant energetic resource which is not efficiently used by the current technologies, therefore, representing an enormous potential for the production of lingocellulosic ethanol. Cellulose can be hydrolyzed in glucose residuals by enzymatic treatment as well as base of acids. Technically, there are some barriers associated to acid and /or enzymatic hydrolysis which need to be overcome. Considering enzymatic hydrolyses, the reaction takes place in a non-aggressive medium, however it takes a long waiting time (from 60 to 72 hours) to occur and the lignin compound is one of the main obstacles for the enzyme access to the cellulose. Thus, the present work aimed at obtaining progress for researches on cellulosic ethanol from the bagasse and, potentially, from other sugarcane by-products through selection of clones that shows high potential bioconvertibility fibers. The experiment was made up of 286 clones and 2 witnesses (RB867515 and SP80-1842) in 25 blocks. They were selected from 13 families of half-siblings and they were vegetative propagation first generation clones. Accordingly, it was carried out correlations among desirable biochemical and agronomical traits, evaluating the cause and effect relationship on fibers and lignin of the stem. It was also estimated the possibility on defining characters which have to be used to carry out a recurrent selection program on sugarcane aiming at alcohol production. It was selected 10 clones with highest lignin content and 10 clones with the lowest lignin content in the bagasse. Those 20 clones were evaluated for the possibility in obtaining a higher glucose content after enzymatic hydrolysis process for cellulosic alcohol production. It could be observed that the higher the content of lignin in the bagasse, the lower the content of glucose produced at the end of hydrolyzesprocess. It was also observed that hemicellulose is more efficient on the conversion than saccharification. Hemicellulose functions as a positive factor on fermentable sugar production for fermentation, because it breaks down cellulose structure allowing access of the enzymes and because of its rupture it supplies glucose molecules for fermentation. Therefore, selected clones show a high potential use on sugar cane genetic breeding, even though it is still needed phenotypic and gene expression analysis studies, enzymatic quantification and syntheses of metabolic, correlating them to syntheses of lignin.Um modelo de matriz energética mundial, idealmente baseado em fontes mais limpas e renováveis de energia em relação ao petróleo, se justifica pela crescente preocupação com a questão do aquecimento global. Além disso, pesquisas em biocombustíveis como fontes alternativas de energia são essenciais para garantir a atual posição de autonomia energética brasileira, além do interesse pela produção de excedentes para garantir a exportação e geração de divisas. Celulose é a biomassa renovável mais abundante na face da terra e por isso, uma boa alternativa energética em relação ao petróleo. Cana de açúcar apresenta na sua massa 2/3 de sua massa em material ligno-celulósico, um recurso energético abundante não utilizado eficientemente por meio das tecnologias atuais, representando portanto, um enorme potencial para a produção de etanol ligno-celulósico. A celulose pode ser hidrolisada em resíduos de glicose tanto por tratamento enzimático ou a base de ácidos. Há inconvenientes associados à hidrólise ácida e/ou enzimática do ponto de vista técnico e que precisam ser superados. No caso da hidrólise enzimática, a reação ocorre em meio não agressivo, porém, requer um longo tempo de espera (60 a 72 horas) e a componente lignina apresenta-se como um dos principais barreiras para o acesso da enzima a celulose. Portanto, o presente trabalho teve como objetivo obter avanços nas pesquisas em etanol celulósico a partir do bagaço e, potencialmente, outros resíduos provenientes do processamento da cana-de-açúcar, por meio da seleção de clones que apresentem fibra com elevado potencial de bioconversibilidade. O experimento foi composto por 286 clones e 2 testemunhas (RB867515 e SP80-1842) em 25 blocos. Tais clones foram selecionados de 13 famílias de meios-irmãos e constituíram clones de primeira geração de propagação vegetativa. Para isso efetuou correlações entre características agronômicas e bioquímicas desejáveis, avaliando a relação causa e efeito sobre o fibras e lignina dos colmos. Também estimou-se a possibilidade de definir os caracteres que deverem ser empregados para conduzir um programa de seleção recorrente em cana-de-açúcar visando à produção de álcool celulósico. Dos clones foram selecionas os 10 que apresentaram maior teor de lignina e os 10 que apresentaram menor teor de lignina no bagaço de cana. Os 20 clones foram avaliados quanta a possibilidade de se obter um maior teor de glicose após o processo de hidrólise enzimática para produção de álcool celulósico. Foi possível observar que quanto maior o teor de lignina no bagaço menor o teor de glicose produzida ao final do processo de hidrólise. Também foi observado que o caráter hemicelulose teve maior correlação com a sacarificação. A hemicelulose funciona como um fator positivo na produção de açúcares fermentáveis para a fermentação, pois abre a estrutura da celulose permitindo acesso enzimático e pela sua quebra fornece moléculas de glicose para a fermentação. Portanto os clones selecionados apresentam potencial para aplicação no melhoramento genético da cana de-açúcar, mesmo que ainda sejam necessários estudos complementares fenotípicos e de análise expressão gênica, quantificação enzimática e síntese de metabólicos, correlacionando-os com a síntese de lignina.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MeSacarificaçãoLigninaCloneAnálise de trilhaCorrelaçõesSaccharumSaccharificationLigninClonePath analysisCorrelationsSaccharumCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALHerdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcarHeritability and genotypic correlations of agronomic traits, cell wall constituents and saccharification in cane sugarinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1568529https://locus.ufv.br//bitstream/123456789/4735/1/texto%20completo.pdf147a0674e2727bd463b090587ba885b5MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain111814https://locus.ufv.br//bitstream/123456789/4735/2/texto%20completo.pdf.txt3d1f4e0ab56b881fed818720f25fa7e8MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3648https://locus.ufv.br//bitstream/123456789/4735/3/texto%20completo.pdf.jpg801567d72053aced8ef7b84a4429567dMD53123456789/47352016-04-10 23:03:27.365oai:locus.ufv.br:123456789/4735Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:03:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
dc.title.alternative.eng.fl_str_mv |
Heritability and genotypic correlations of agronomic traits, cell wall constituents and saccharification in cane sugar |
title |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
spellingShingle |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar Baffa, David Carlos Ferreira Sacarificação Lignina Clone Análise de trilha Correlações Saccharum Saccharification Lignin Clone Path analysis Correlations Saccharum CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
title_short |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
title_full |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
title_fullStr |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
title_full_unstemmed |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
title_sort |
Herdabilidade e correlações genotípicas de caracteres agronômicos, constituintes da parede celular e sacarificação em cana-de-açúcar |
author |
Baffa, David Carlos Ferreira |
author_facet |
Baffa, David Carlos Ferreira |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1624329861472308 |
dc.contributor.author.fl_str_mv |
Baffa, David Carlos Ferreira |
dc.contributor.advisor-co1.fl_str_mv |
Loureiro, Marcelo Ehlers |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3 |
dc.contributor.advisor-co2.fl_str_mv |
Peternelli, Luiz Alexandre |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723301Z7 |
dc.contributor.advisor1.fl_str_mv |
Barbosa, Marcio Henrique Pereira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782585E6 |
dc.contributor.referee1.fl_str_mv |
Diola, Valdir |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4133062U6 |
dc.contributor.referee2.fl_str_mv |
Lopes, Francis Julio Fagundes |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762375P5 |
contributor_str_mv |
Loureiro, Marcelo Ehlers Peternelli, Luiz Alexandre Barbosa, Marcio Henrique Pereira Diola, Valdir Lopes, Francis Julio Fagundes |
dc.subject.por.fl_str_mv |
Sacarificação Lignina Clone Análise de trilha Correlações Saccharum |
topic |
Sacarificação Lignina Clone Análise de trilha Correlações Saccharum Saccharification Lignin Clone Path analysis Correlations Saccharum CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
dc.subject.eng.fl_str_mv |
Saccharification Lignin Clone Path analysis Correlations Saccharum |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
description |
A model of an international energy matrix, ideally based on renewable and cleaner energy regarded to petrol, is justified because of the increasing awareness on the global warming issue. Moreover, researches on biofuel, as an alternative source of energy, are essential to guarantee the current ranking of the Brazilian energy autonomy, in addition to the interest in production of surplus so to guarantee exportation and generation of foreign exchange. Cellulose is the most abundant renewable biomass in the planet, therefore a good energetic alternative to petrol. Sugarcane has 2/3 of its mass in lingocellulosic material, an abundant energetic resource which is not efficiently used by the current technologies, therefore, representing an enormous potential for the production of lingocellulosic ethanol. Cellulose can be hydrolyzed in glucose residuals by enzymatic treatment as well as base of acids. Technically, there are some barriers associated to acid and /or enzymatic hydrolysis which need to be overcome. Considering enzymatic hydrolyses, the reaction takes place in a non-aggressive medium, however it takes a long waiting time (from 60 to 72 hours) to occur and the lignin compound is one of the main obstacles for the enzyme access to the cellulose. Thus, the present work aimed at obtaining progress for researches on cellulosic ethanol from the bagasse and, potentially, from other sugarcane by-products through selection of clones that shows high potential bioconvertibility fibers. The experiment was made up of 286 clones and 2 witnesses (RB867515 and SP80-1842) in 25 blocks. They were selected from 13 families of half-siblings and they were vegetative propagation first generation clones. Accordingly, it was carried out correlations among desirable biochemical and agronomical traits, evaluating the cause and effect relationship on fibers and lignin of the stem. It was also estimated the possibility on defining characters which have to be used to carry out a recurrent selection program on sugarcane aiming at alcohol production. It was selected 10 clones with highest lignin content and 10 clones with the lowest lignin content in the bagasse. Those 20 clones were evaluated for the possibility in obtaining a higher glucose content after enzymatic hydrolysis process for cellulosic alcohol production. It could be observed that the higher the content of lignin in the bagasse, the lower the content of glucose produced at the end of hydrolyzesprocess. It was also observed that hemicellulose is more efficient on the conversion than saccharification. Hemicellulose functions as a positive factor on fermentable sugar production for fermentation, because it breaks down cellulose structure allowing access of the enzymes and because of its rupture it supplies glucose molecules for fermentation. Therefore, selected clones show a high potential use on sugar cane genetic breeding, even though it is still needed phenotypic and gene expression analysis studies, enzymatic quantification and syntheses of metabolic, correlating them to syntheses of lignin. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-07-22 |
dc.date.available.fl_str_mv |
2012-03-26 2015-03-26T13:42:18Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:42:18Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BAFFA, David Carlos Ferreira. Heritability and genotypic correlations of agronomic traits, cell wall constituents and saccharification in cane sugar. 2010. 65 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2010. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4735 |
identifier_str_mv |
BAFFA, David Carlos Ferreira. Heritability and genotypic correlations of agronomic traits, cell wall constituents and saccharification in cane sugar. 2010. 65 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2010. |
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http://locus.ufv.br/handle/123456789/4735 |
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Universidade Federal de Viçosa |
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Mestrado em Genética e Melhoramento |
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UFV |
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BR |
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Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me |
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Universidade Federal de Viçosa |
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