Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos

• Autor(a) principal: Santos, Giancarlo Magalhães dos
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4960
• topic: Bovinos; Ovócitos; FIV; Bovine; Oocytes; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL

The objective of this work was to assess and compare the influence of different expositon time and transportation media on in vitro maturation of immature bovine ocytes. We used 799 immature oocytes, distributed in ten treatments, one of them, the control. Three transportation media were tested: Talp-Hepes, according to BAVISTER et al. (1983); modified Talp-Hepes, with 10 µg/mL of FSH and 0,0454 mM of Sodium Piruvate and modified TCM without NaHCO3, with 5,665 mM of Hepes, 0,999 mM of NaCl, 0,0412 mM of Calcium Lactate, 0,0454 mM of Sodium Piruvate, 10.000 UI of Sodic Penicillin G, 0,005g% of Streptomycin, 0,4g% of BSA and 10 µg/mL of FSH. In each of the used media, the oocyte were kept on a plate and heated at 38oC for two, four and eight hours. After this time, the oocytes were submitted to in vitro cultivation in TCM 199 media with 10% of SVE and 10 µg/mL of FSH, in CO2 oven, for 24 hours, for later nuclear maturation rate evaluation. In the control, after the oocyte decanting and selection, they were immediately submitted to in vitro cultivation procedure. We observed that in the Talp-Hepes treatment with two, four and eight hours, the nuclear maturation rates were 84,7; 81,2 and 81,3%, respectively. In the modified Talp-Hepes media the rates were 72,2; 82,0 and 82,2%, and the modified TCM media the rates were 80,0; 78,6 and 80,0%, respectively to two, four and eight hours. In the control we observed 80,1% in metaphase II stage. Concluding, the transportation media and the time between the collection and the incubation for in vitro maturation didn t influence (P>0,05) the nuclear maturation rate. So, these media can be a possible alternative to the bovine oocytes transportation for in vitro embryo production.